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Rapid high voltage isoelectric focusing of proteins in rod gels.
Biomed Chromatogr. 1991 Sep; 5(5):221-5.BC

Abstract

A rapid procedure of isoelectric focusing (IEF) of proteins in polyacrylamide rod gels (i.d., 1.1 mm; length, 7.5 cm) is described. The time required for IEF can be reduced to 0.5 h by using high voltages up to 3000 V in the presence or absence of urea in the gels. When used as the first dimension of a two-dimensional technique for IEF sodium dodecyl sulphate electrophoresis, high voltage IEF gives smaller protein spots on the second dimension gel, associated with an increase in resolution. The method has been tested by a two-dimensional separation of an eye sample of the goodeid fish Xenotoca eiseni.

Authors+Show Affiliations

Das J
Biochemisches Institut, Medizinischen Fakultät, Christian-Albrechts-Universität, Kiel, Germany.

MeSH

AnimalsCytochrome c GroupElectrophoresis, Gel, Two-DimensionalEyeFishesHemoglobinsHydrogen-Ion ConcentrationIsoelectric FocusingMyoglobinProteinsUrea

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1660334

Citation

Das, J. "Rapid High Voltage Isoelectric Focusing of Proteins in Rod Gels." Biomedical Chromatography : BMC, vol. 5, no. 5, 1991, pp. 221-5.
Das J. Rapid high voltage isoelectric focusing of proteins in rod gels. Biomed Chromatogr. 1991;5(5):221-5.
Das, J. (1991). Rapid high voltage isoelectric focusing of proteins in rod gels. Biomedical Chromatography : BMC, 5(5), 221-5.
Das J. Rapid High Voltage Isoelectric Focusing of Proteins in Rod Gels. Biomed Chromatogr. 1991;5(5):221-5. PubMed PMID: 1660334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid high voltage isoelectric focusing of proteins in rod gels. A1 - Das,J, PY - 1991/9/1/pubmed PY - 1991/9/1/medline PY - 1991/9/1/entrez SP - 221 EP - 5 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 5 IS - 5 N2 - A rapid procedure of isoelectric focusing (IEF) of proteins in polyacrylamide rod gels (i.d., 1.1 mm; length, 7.5 cm) is described. The time required for IEF can be reduced to 0.5 h by using high voltages up to 3000 V in the presence or absence of urea in the gels. When used as the first dimension of a two-dimensional technique for IEF sodium dodecyl sulphate electrophoresis, high voltage IEF gives smaller protein spots on the second dimension gel, associated with an increase in resolution. The method has been tested by a two-dimensional separation of an eye sample of the goodeid fish Xenotoca eiseni. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/1660334/Rapid_high_voltage_isoelectric_focusing_of_proteins_in_rod_gels_ DB - PRIME DP - Unbound Medicine ER -