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Analysis of the genomic response of a wine yeast to rehydration and inoculation.
Appl Microbiol Biotechnol. 2006 Aug; 71(5):699-712.AM

Abstract

We used DNA microarrays to study the transcriptome of a wine yeast before and after rehydration and during the first hours following inoculation of a synthetic must. There was a substantial transcriptional remodeling during this period, including 1,874 genes regulated more than threefold. Dried yeasts displayed an expression profile typical of respiratory-grown cells starved for nitrogen and carbon and which had been highly stressed. During rehydration, many genes involved in biosynthetic pathways, in transcription or in protein synthesis were coordinately induced while genes subject to glucose repression were down-regulated. The transcriptional response was very rapid indicating that yeast quickly recovered the capacity to sense environmental signals and to respond appropriately. Our data show that genes involved in the general stress response were repressed during rehydration while acid stress specific genes were induced probably in response to organic acid accumulation. The glycolytic genes and acid stress-responsive genes were simultaneously and transiently repressed after inoculation into the fermentation medium suggesting that regulation of glycolytic genes may correspond to an adjustment to the energetic needs of the cells. Surprisingly, inoculation into the must did not trigger a stress response despite the high concentrations of sugars.

Authors+Show Affiliations

Rossignol T
Equipe de Microbiologie, UMR Sciences Pour l'Oenologie, INRA-ENSAM-UMI, 2 place Viala, Montpellier 34060, Cedex 1, France.
Postaire O
No affiliation info available
Storaï J
No affiliation info available
Blondin B
No affiliation info available

MeSH

Culture MediaFermentationFungal ProteinsGene Expression ProfilingGene Expression Regulation, FungalGenome, FungalHeat-Shock ResponseProteomeSaccharomyces cerevisiaeWineYeast, Dried

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16607525

Citation

Rossignol, Tristan, et al. "Analysis of the Genomic Response of a Wine Yeast to Rehydration and Inoculation." Applied Microbiology and Biotechnology, vol. 71, no. 5, 2006, pp. 699-712.
Rossignol T, Postaire O, Storaï J, et al. Analysis of the genomic response of a wine yeast to rehydration and inoculation. Appl Microbiol Biotechnol. 2006;71(5):699-712.
Rossignol, T., Postaire, O., Storaï, J., & Blondin, B. (2006). Analysis of the genomic response of a wine yeast to rehydration and inoculation. Applied Microbiology and Biotechnology, 71(5), 699-712.
Rossignol T, et al. Analysis of the Genomic Response of a Wine Yeast to Rehydration and Inoculation. Appl Microbiol Biotechnol. 2006;71(5):699-712. PubMed PMID: 16607525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the genomic response of a wine yeast to rehydration and inoculation. AU - Rossignol,Tristan, AU - Postaire,Olivier, AU - Storaï,Julien, AU - Blondin,Bruno, Y1 - 2006/04/11/ PY - 2006/01/04/received PY - 2006/03/02/accepted PY - 2006/02/06/revised PY - 2006/4/12/pubmed PY - 2006/10/18/medline PY - 2006/4/12/entrez SP - 699 EP - 712 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 71 IS - 5 N2 - We used DNA microarrays to study the transcriptome of a wine yeast before and after rehydration and during the first hours following inoculation of a synthetic must. There was a substantial transcriptional remodeling during this period, including 1,874 genes regulated more than threefold. Dried yeasts displayed an expression profile typical of respiratory-grown cells starved for nitrogen and carbon and which had been highly stressed. During rehydration, many genes involved in biosynthetic pathways, in transcription or in protein synthesis were coordinately induced while genes subject to glucose repression were down-regulated. The transcriptional response was very rapid indicating that yeast quickly recovered the capacity to sense environmental signals and to respond appropriately. Our data show that genes involved in the general stress response were repressed during rehydration while acid stress specific genes were induced probably in response to organic acid accumulation. The glycolytic genes and acid stress-responsive genes were simultaneously and transiently repressed after inoculation into the fermentation medium suggesting that regulation of glycolytic genes may correspond to an adjustment to the energetic needs of the cells. Surprisingly, inoculation into the must did not trigger a stress response despite the high concentrations of sugars. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/16607525/Analysis_of_the_genomic_response_of_a_wine_yeast_to_rehydration_and_inoculation_ DB - PRIME DP - Unbound Medicine ER -