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In vitro cytotoxicity of nonpolar constituents from different parts of kava plant (Piper methysticum).
J Agric Food Chem. 2006 Apr 19; 54(8):3157-62.JA

Abstract

Kava (Piper methysticum), a perennial shrub native to the South Pacific islands, has been used to relieve anxiety. Recently, several cases of severe hepatotoxicity have been reported from the consumption of dietary supplements containing kava. It is unclear whether the kava constituents, kavalactones, are responsible for the associated hepatotoxicity. To investigate the key components responsible for the liver toxicity, bioassay-guided fractionation was carried out in this study. Kava roots, leaves, and stem peelings were extracted with methanol, and the resulting residues were subjected to partition with a different polarity of solvents (hexane, ethyl acetate, n-butanol, and water) for evaluation of their cytotoxicity on HepG2 cells based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and lactate dehydrogenase and aspartate aminotransferase enzyme leakage assays. Organic solvent fractions displayed a much stronger cytotoxicity than water fractions for all parts of kava. The hexane fraction of the root exhibited stronger cytotoxic effects than fractions of root extracted with other solvents or extracts from the other parts of kava. Further investigations using bioassay-directed isolation and analysis of the hexane fraction indicated that the compound responsible for the cytotoxicity was flavokavain B. The identity of the compound was confirmed by (1)H and (13) C NMR and MS techniques.

Authors+Show Affiliations

National Center for Toxicological Research, U.S. Food and Drug Administration, HFT-230, 3900 NCTR Road, Jefferson, Arkansas 72079, USA. jjhoo@kangwon.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16608246

Citation

Jhoo, Jin-Woo, et al. "In Vitro Cytotoxicity of Nonpolar Constituents From Different Parts of Kava Plant (Piper Methysticum)." Journal of Agricultural and Food Chemistry, vol. 54, no. 8, 2006, pp. 3157-62.
Jhoo JW, Freeman JP, Heinze TM, et al. In vitro cytotoxicity of nonpolar constituents from different parts of kava plant (Piper methysticum). J Agric Food Chem. 2006;54(8):3157-62.
Jhoo, J. W., Freeman, J. P., Heinze, T. M., Moody, J. D., Schnackenberg, L. K., Beger, R. D., Dragull, K., Tang, C. S., & Ang, C. Y. (2006). In vitro cytotoxicity of nonpolar constituents from different parts of kava plant (Piper methysticum). Journal of Agricultural and Food Chemistry, 54(8), 3157-62.
Jhoo JW, et al. In Vitro Cytotoxicity of Nonpolar Constituents From Different Parts of Kava Plant (Piper Methysticum). J Agric Food Chem. 2006 Apr 19;54(8):3157-62. PubMed PMID: 16608246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro cytotoxicity of nonpolar constituents from different parts of kava plant (Piper methysticum). AU - Jhoo,Jin-Woo, AU - Freeman,James P, AU - Heinze,Thomas M, AU - Moody,Joanna D, AU - Schnackenberg,Laura K, AU - Beger,Richard D, AU - Dragull,Klaus, AU - Tang,Chung-Shih, AU - Ang,Catharina Y W, PY - 2006/4/13/pubmed PY - 2006/6/21/medline PY - 2006/4/13/entrez SP - 3157 EP - 62 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 8 N2 - Kava (Piper methysticum), a perennial shrub native to the South Pacific islands, has been used to relieve anxiety. Recently, several cases of severe hepatotoxicity have been reported from the consumption of dietary supplements containing kava. It is unclear whether the kava constituents, kavalactones, are responsible for the associated hepatotoxicity. To investigate the key components responsible for the liver toxicity, bioassay-guided fractionation was carried out in this study. Kava roots, leaves, and stem peelings were extracted with methanol, and the resulting residues were subjected to partition with a different polarity of solvents (hexane, ethyl acetate, n-butanol, and water) for evaluation of their cytotoxicity on HepG2 cells based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and lactate dehydrogenase and aspartate aminotransferase enzyme leakage assays. Organic solvent fractions displayed a much stronger cytotoxicity than water fractions for all parts of kava. The hexane fraction of the root exhibited stronger cytotoxic effects than fractions of root extracted with other solvents or extracts from the other parts of kava. Further investigations using bioassay-directed isolation and analysis of the hexane fraction indicated that the compound responsible for the cytotoxicity was flavokavain B. The identity of the compound was confirmed by (1)H and (13) C NMR and MS techniques. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16608246/In_vitro_cytotoxicity_of_nonpolar_constituents_from_different_parts_of_kava_plant__Piper_methysticum__ L2 - https://doi.org/10.1021/jf051853j DB - PRIME DP - Unbound Medicine ER -