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Identification and characterization of the carboxy-terminal region of Sip-1, a novel autoantigen in Behçet's disease.
Arthritis Res Ther. 2006; 8(3):R71.AR

Abstract

Given the lack of a serological test specific for Behçet's disease, its diagnosis rests upon clinical criteria. The clinical diagnosis is nevertheless difficult because the disease manifestations vary widely, especially at the onset of disease. The aim of this study was to identify molecules specifically recognized by serum autoantibodies in patients with Behçet's disease and to evaluate their diagnostic value. We screened a cDNA library from human microvascular endothelial cells with serum IgG from two patients with Behçet's disease and isolated a reactive clone specific to the carboxy-terminal subunit of Sip1 (Sip1 C-ter). Using ELISA, we measured IgG, IgM and IgA specific to Sip1 C-ter in patients with various autoimmune diseases characterized by the presence of serum anti-endothelial cell antibodies, such as Behçet's disease, systemic lupus erythematosus, systemic sclerosis and various forms of primary vasculitis, as well as in patients with diseases that share clinical features with Behçet's disease, such as inflammatory bowel disease and uveitis. IgM immunoreactivity to Sip1 C-ter was significantly higher in patients with Behçet's disease and in patients with primary vasculitis than in the other groups of patients and healthy subjects tested (P < 10-4 by Mann-Whitney test). ELISA detected IgG specific to Sip1 C-ter in sera from 11/56 (20%) patients with Behçet's disease, IgM in 23/56 (41%) and IgA in 9/54 (17%). No sera from patients with systemic lupus erythematosus, systemic sclerosis, inflammatory bowel disease, uveitis or healthy subjects but 45% of sera from patients with primary vasculitis contained IgM specific to Sip1 C-ter. Serum levels of soluble E-selectin, a marker of endothelial activation and inflammation, correlated with levels of serum IgM anti Sip-1 C-ter in patients with Behçet's disease (r = 0.36, P = 0.023). In conclusion, Sip1 C-ter is a novel autoantigen in Behçet's disease. IgM specific to Sip1 C-ter might be useful in clinical practice as an immunological marker of endothelial dysfunction in vasculitis.

Authors+Show Affiliations

Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16611372

Citation

Delunardo, Federica, et al. "Identification and Characterization of the Carboxy-terminal Region of Sip-1, a Novel Autoantigen in Behçet's Disease." Arthritis Research & Therapy, vol. 8, no. 3, 2006, pp. R71.
Delunardo F, Conti F, Margutti P, et al. Identification and characterization of the carboxy-terminal region of Sip-1, a novel autoantigen in Behçet's disease. Arthritis Res Ther. 2006;8(3):R71.
Delunardo, F., Conti, F., Margutti, P., Alessandri, C., Priori, R., Siracusano, A., Riganò, R., Profumo, E., Valesini, G., Sorice, M., & Ortona, E. (2006). Identification and characterization of the carboxy-terminal region of Sip-1, a novel autoantigen in Behçet's disease. Arthritis Research & Therapy, 8(3), R71.
Delunardo F, et al. Identification and Characterization of the Carboxy-terminal Region of Sip-1, a Novel Autoantigen in Behçet's Disease. Arthritis Res Ther. 2006;8(3):R71. PubMed PMID: 16611372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of the carboxy-terminal region of Sip-1, a novel autoantigen in Behçet's disease. AU - Delunardo,Federica, AU - Conti,Fabrizio, AU - Margutti,Paola, AU - Alessandri,Cristiano, AU - Priori,Roberta, AU - Siracusano,Alessandra, AU - Riganò,Rachele, AU - Profumo,Elisabetta, AU - Valesini,Guido, AU - Sorice,Maurizio, AU - Ortona,Elena, Y1 - 2006/04/12/ PY - 2005/11/24/received PY - 2006/02/23/revised PY - 2006/03/17/accepted PY - 2006/4/14/pubmed PY - 2006/8/3/medline PY - 2006/4/14/entrez SP - R71 EP - R71 JF - Arthritis research & therapy JO - Arthritis Res Ther VL - 8 IS - 3 N2 - Given the lack of a serological test specific for Behçet's disease, its diagnosis rests upon clinical criteria. The clinical diagnosis is nevertheless difficult because the disease manifestations vary widely, especially at the onset of disease. The aim of this study was to identify molecules specifically recognized by serum autoantibodies in patients with Behçet's disease and to evaluate their diagnostic value. We screened a cDNA library from human microvascular endothelial cells with serum IgG from two patients with Behçet's disease and isolated a reactive clone specific to the carboxy-terminal subunit of Sip1 (Sip1 C-ter). Using ELISA, we measured IgG, IgM and IgA specific to Sip1 C-ter in patients with various autoimmune diseases characterized by the presence of serum anti-endothelial cell antibodies, such as Behçet's disease, systemic lupus erythematosus, systemic sclerosis and various forms of primary vasculitis, as well as in patients with diseases that share clinical features with Behçet's disease, such as inflammatory bowel disease and uveitis. IgM immunoreactivity to Sip1 C-ter was significantly higher in patients with Behçet's disease and in patients with primary vasculitis than in the other groups of patients and healthy subjects tested (P < 10-4 by Mann-Whitney test). ELISA detected IgG specific to Sip1 C-ter in sera from 11/56 (20%) patients with Behçet's disease, IgM in 23/56 (41%) and IgA in 9/54 (17%). No sera from patients with systemic lupus erythematosus, systemic sclerosis, inflammatory bowel disease, uveitis or healthy subjects but 45% of sera from patients with primary vasculitis contained IgM specific to Sip1 C-ter. Serum levels of soluble E-selectin, a marker of endothelial activation and inflammation, correlated with levels of serum IgM anti Sip-1 C-ter in patients with Behçet's disease (r = 0.36, P = 0.023). In conclusion, Sip1 C-ter is a novel autoantigen in Behçet's disease. IgM specific to Sip1 C-ter might be useful in clinical practice as an immunological marker of endothelial dysfunction in vasculitis. SN - 1478-6362 UR - https://www.unboundmedicine.com/medline/citation/16611372/Identification_and_characterization_of_the_carboxy_terminal_region_of_Sip_1_a_novel_autoantigen_in_Behçet's_disease_ L2 - https://arthritis-research.biomedcentral.com/articles/10.1186/ar1940 DB - PRIME DP - Unbound Medicine ER -