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Differential densities of muscarinic acetylcholine receptor and I(K,ACh) in canine supraventricular tissues and the effect of amiodarone on cholinergic atrial fibrillation and I(K,ACh).
Cardiology. 2006; 106(1):36-43.C

Abstract

BACKGROUND

Vagal nerve plays an important role in the induction and maintenance of atrial fibrillation (AF). This study investigated the differential densities of M2 receptor and acetylcholine-induced inward rectifier K+ current (I(K,ACh)) in atrial appendage, atrium, pulmonary vein (PV) and super vena cava (SVC) to discuss the role of atrial appendage and PV in cholinergic AF.

METHODS AND RESULTS

In 10 dogs, action potential duration was determined at 24 sites during bilateral cervical vagal stimulation and amiodarone administration. AF could be induced at first in right atrial appendage (RAA) and right atrium (RA) without left atrial appendage (LAA) and left atrium (LA). Amiodarone decreased the initiation of AF in vivo. Western blot and patch clamp were used to determine M2 receptor and I(K,ACh) in RAA, LAA, RA, LA, PV and SVC. The densities of M2 receptor and I(K,ACh) in LAA, RAA and LA were higher than that in RA, PV and SVC (21.34 +/- 0.92 vs. 8.24 +/- 0.45 pA/pF, p < 0.05). Furthermore, the densities of the M2 receptor and I(K,ACh) in LAA and RAA were higher than that in LA (21.34 +/- 0.92 vs. 14.17 +/- 0.65 pA/pF, p < 0.05). After amiodarone administration, densities of I(K,ACh) in LA and RA were not different, but densities of I(K,ACh)were also less in atrium than in atrial appendage.

CONCLUSIONS

Densities of the M2 receptor and I(K,ACh) are higher in atrial appendage than other sites. Atrial appendage perhaps plays an important role in initiation of cholinergic AF. However, PV and SVC less often play an important role in vagotonic paroxysmal AF. Reduced dispersion of I(K,ACh) is the mechanism for amiodarone to therapy AF.

Authors+Show Affiliations

Huang CX
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China. ruyan1971@yahoo.com.cn
Zhao QY
No affiliation info available
Liang JJ
No affiliation info available
Chen H
No affiliation info available
Yang B
No affiliation info available
Jiang H
No affiliation info available
Li GS
No affiliation info available

MeSH

Action PotentialsAmiodaroneAnimalsAnti-Arrhythmia AgentsAtrial AppendageAtrial FibrillationDogsElectric StimulationHeart AtriaPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingPulmonary VeinsReceptors, MuscarinicVagus NerveVena Cava, Superior

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16612067

Citation

Huang, Cong-Xin, et al. "Differential Densities of Muscarinic Acetylcholine Receptor and I(K,ACh) in Canine Supraventricular Tissues and the Effect of Amiodarone On Cholinergic Atrial Fibrillation and I(K,ACh)." Cardiology, vol. 106, no. 1, 2006, pp. 36-43.
Huang CX, Zhao QY, Liang JJ, et al. Differential densities of muscarinic acetylcholine receptor and I(K,ACh) in canine supraventricular tissues and the effect of amiodarone on cholinergic atrial fibrillation and I(K,ACh). Cardiology. 2006;106(1):36-43.
Huang, C. X., Zhao, Q. Y., Liang, J. J., Chen, H., Yang, B., Jiang, H., & Li, G. S. (2006). Differential densities of muscarinic acetylcholine receptor and I(K,ACh) in canine supraventricular tissues and the effect of amiodarone on cholinergic atrial fibrillation and I(K,ACh). Cardiology, 106(1), 36-43.
Huang CX, et al. Differential Densities of Muscarinic Acetylcholine Receptor and I(K,ACh) in Canine Supraventricular Tissues and the Effect of Amiodarone On Cholinergic Atrial Fibrillation and I(K,ACh). Cardiology. 2006;106(1):36-43. PubMed PMID: 16612067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential densities of muscarinic acetylcholine receptor and I(K,ACh) in canine supraventricular tissues and the effect of amiodarone on cholinergic atrial fibrillation and I(K,ACh). AU - Huang,Cong-Xin, AU - Zhao,Qing-Yan, AU - Liang,Jin-Jun, AU - Chen,Hui, AU - Yang,Bo, AU - Jiang,Hong, AU - Li,Geng-Shan, Y1 - 2006/04/10/ PY - 2005/10/20/received PY - 2006/01/24/accepted PY - 2006/4/14/pubmed PY - 2006/8/23/medline PY - 2006/4/14/entrez SP - 36 EP - 43 JF - Cardiology JO - Cardiology VL - 106 IS - 1 N2 - BACKGROUND: Vagal nerve plays an important role in the induction and maintenance of atrial fibrillation (AF). This study investigated the differential densities of M2 receptor and acetylcholine-induced inward rectifier K+ current (I(K,ACh)) in atrial appendage, atrium, pulmonary vein (PV) and super vena cava (SVC) to discuss the role of atrial appendage and PV in cholinergic AF. METHODS AND RESULTS: In 10 dogs, action potential duration was determined at 24 sites during bilateral cervical vagal stimulation and amiodarone administration. AF could be induced at first in right atrial appendage (RAA) and right atrium (RA) without left atrial appendage (LAA) and left atrium (LA). Amiodarone decreased the initiation of AF in vivo. Western blot and patch clamp were used to determine M2 receptor and I(K,ACh) in RAA, LAA, RA, LA, PV and SVC. The densities of M2 receptor and I(K,ACh) in LAA, RAA and LA were higher than that in RA, PV and SVC (21.34 +/- 0.92 vs. 8.24 +/- 0.45 pA/pF, p < 0.05). Furthermore, the densities of the M2 receptor and I(K,ACh) in LAA and RAA were higher than that in LA (21.34 +/- 0.92 vs. 14.17 +/- 0.65 pA/pF, p < 0.05). After amiodarone administration, densities of I(K,ACh) in LA and RA were not different, but densities of I(K,ACh)were also less in atrium than in atrial appendage. CONCLUSIONS: Densities of the M2 receptor and I(K,ACh) are higher in atrial appendage than other sites. Atrial appendage perhaps plays an important role in initiation of cholinergic AF. However, PV and SVC less often play an important role in vagotonic paroxysmal AF. Reduced dispersion of I(K,ACh) is the mechanism for amiodarone to therapy AF. SN - 0008-6312 UR - https://www.unboundmedicine.com/medline/citation/16612067/Differential_densities_of_muscarinic_acetylcholine_receptor_and_I_KACh__in_canine_supraventricular_tissues_and_the_effect_of_amiodarone_on_cholinergic_atrial_fibrillation_and_I_KACh__ DB - PRIME DP - Unbound Medicine ER -