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Differential regulation of dentin matrix protein 1 expression during odontogenesis.
Cells Tissues Organs 2005; 181(3-4):241-7CT

Abstract

Dentin matrix protein 1 (DMP1) is highly expressed in mineralized tooth and bone. Both in vitro and in vivo data show that DMP1 is critical for mineralization and tooth morphogenesis (growth and development). In this study, we studied Dmp1 gene regulation. The in vitro transient transfection assay identified two important DNA fragments, the 2.4- and 9.6-kb promoter regions. We next generated and analyzed transgenic mice bearing the beta-galactosidase (lacZ) reporter gene driven by the 2.4- or 9.6-kb promoter with the complete 4-kb intron 1. The 9.6-kb Dmp1-lacZ mice conferred a DMP1 expression pattern in odontoblasts identical to that in the endogenous Dmp1 gene. This is reflected by lacZ expression in Dmp1-lacZ knock-in mice during all stages of odontogenesis. In contrast, the 2.4-kb Dmp1-lacZ mice display activity in odontoblast cells only at the early stage of odontogenesis. Thus, we propose that different transcription factors regulate early or later cis-regulatory domains of the Dmp1 promoter, which gives rise to the unique spatial and temporal expression pattern of Dmp1 gene at different stages of tooth development.

Authors+Show Affiliations

Department of Oral Biology, School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16612089

Citation

Lu, Yongbo, et al. "Differential Regulation of Dentin Matrix Protein 1 Expression During Odontogenesis." Cells, Tissues, Organs, vol. 181, no. 3-4, 2005, pp. 241-7.
Lu Y, Zhang S, Xie Y, et al. Differential regulation of dentin matrix protein 1 expression during odontogenesis. Cells Tissues Organs (Print). 2005;181(3-4):241-7.
Lu, Y., Zhang, S., Xie, Y., Pi, Y., & Feng, J. Q. (2005). Differential regulation of dentin matrix protein 1 expression during odontogenesis. Cells, Tissues, Organs, 181(3-4), pp. 241-7.
Lu Y, et al. Differential Regulation of Dentin Matrix Protein 1 Expression During Odontogenesis. Cells Tissues Organs (Print). 2005;181(3-4):241-7. PubMed PMID: 16612089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential regulation of dentin matrix protein 1 expression during odontogenesis. AU - Lu,Yongbo, AU - Zhang,Shubin, AU - Xie,Yixia, AU - Pi,Yuli, AU - Feng,Jian Q, PY - 2006/4/14/pubmed PY - 2006/6/9/medline PY - 2006/4/14/entrez SP - 241 EP - 7 JF - Cells, tissues, organs JO - Cells Tissues Organs (Print) VL - 181 IS - 3-4 N2 - Dentin matrix protein 1 (DMP1) is highly expressed in mineralized tooth and bone. Both in vitro and in vivo data show that DMP1 is critical for mineralization and tooth morphogenesis (growth and development). In this study, we studied Dmp1 gene regulation. The in vitro transient transfection assay identified two important DNA fragments, the 2.4- and 9.6-kb promoter regions. We next generated and analyzed transgenic mice bearing the beta-galactosidase (lacZ) reporter gene driven by the 2.4- or 9.6-kb promoter with the complete 4-kb intron 1. The 9.6-kb Dmp1-lacZ mice conferred a DMP1 expression pattern in odontoblasts identical to that in the endogenous Dmp1 gene. This is reflected by lacZ expression in Dmp1-lacZ knock-in mice during all stages of odontogenesis. In contrast, the 2.4-kb Dmp1-lacZ mice display activity in odontoblast cells only at the early stage of odontogenesis. Thus, we propose that different transcription factors regulate early or later cis-regulatory domains of the Dmp1 promoter, which gives rise to the unique spatial and temporal expression pattern of Dmp1 gene at different stages of tooth development. SN - 1422-6405 UR - https://www.unboundmedicine.com/medline/citation/16612089/Differential_regulation_of_dentin_matrix_protein_1_expression_during_odontogenesis_ L2 - https://www.karger.com?DOI=10.1159/000091385 DB - PRIME DP - Unbound Medicine ER -