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Characterization and expression of a cDNA encoding a tubuliform silk protein of the golden web spider Nephila antipodiana.
Biochimie 2006; 88(7):849-58B

Abstract

Spider silks are renowned for their excellent mechanical properties. Although several spider fibroin genes, mainly from dragline and capture silks, have been identified, there are still many members in the spider fibroin gene family remain uncharacterized. In this study, a novel silk cDNA clone from the golden web spider Nephila antipodiana was isolated. It is serine rich and contains two almost identical fragments with one varied gap region and one conserved spider fibroin-like C-terminal domain. Both in situ hybridization and immunoblot analyses have shown that it is specifically expressed in the tubuliform gland. Thus, it likely encodes the silk fibroin from the tubuliform gland, which supplies the main component of the inner egg case. Unlike other silk proteins, the protein encoded by the novel cDNA in water solution exhibits the characteristic of an alpha-helical protein, which implies the distinct property of the egg case silk, though the fiber of tubuliform silk is mainly composed of beta-sheet structure. Its sequence information facilitates elucidation of the evolutionary history of the araneoid fibroin genes.

Authors+Show Affiliations

Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543 Singapore, Singapore.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16616407

Citation

Huang, W, et al. "Characterization and Expression of a cDNA Encoding a Tubuliform Silk Protein of the Golden Web Spider Nephila Antipodiana." Biochimie, vol. 88, no. 7, 2006, pp. 849-58.
Huang W, Lin Z, Sin YM, et al. Characterization and expression of a cDNA encoding a tubuliform silk protein of the golden web spider Nephila antipodiana. Biochimie. 2006;88(7):849-58.
Huang, W., Lin, Z., Sin, Y. M., Li, D., Gong, Z., & Yang, D. (2006). Characterization and expression of a cDNA encoding a tubuliform silk protein of the golden web spider Nephila antipodiana. Biochimie, 88(7), pp. 849-58.
Huang W, et al. Characterization and Expression of a cDNA Encoding a Tubuliform Silk Protein of the Golden Web Spider Nephila Antipodiana. Biochimie. 2006;88(7):849-58. PubMed PMID: 16616407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and expression of a cDNA encoding a tubuliform silk protein of the golden web spider Nephila antipodiana. AU - Huang,W, AU - Lin,Z, AU - Sin,Y M, AU - Li,D, AU - Gong,Z, AU - Yang,D, Y1 - 2006/03/29/ PY - 2005/08/26/received PY - 2006/02/04/accepted PY - 2006/4/18/pubmed PY - 2006/9/27/medline PY - 2006/4/18/entrez SP - 849 EP - 58 JF - Biochimie JO - Biochimie VL - 88 IS - 7 N2 - Spider silks are renowned for their excellent mechanical properties. Although several spider fibroin genes, mainly from dragline and capture silks, have been identified, there are still many members in the spider fibroin gene family remain uncharacterized. In this study, a novel silk cDNA clone from the golden web spider Nephila antipodiana was isolated. It is serine rich and contains two almost identical fragments with one varied gap region and one conserved spider fibroin-like C-terminal domain. Both in situ hybridization and immunoblot analyses have shown that it is specifically expressed in the tubuliform gland. Thus, it likely encodes the silk fibroin from the tubuliform gland, which supplies the main component of the inner egg case. Unlike other silk proteins, the protein encoded by the novel cDNA in water solution exhibits the characteristic of an alpha-helical protein, which implies the distinct property of the egg case silk, though the fiber of tubuliform silk is mainly composed of beta-sheet structure. Its sequence information facilitates elucidation of the evolutionary history of the araneoid fibroin genes. SN - 0300-9084 UR - https://www.unboundmedicine.com/medline/citation/16616407/Characterization_and_expression_of_a_cDNA_encoding_a_tubuliform_silk_protein_of_the_golden_web_spider_Nephila_antipodiana_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-9084(06)00014-9 DB - PRIME DP - Unbound Medicine ER -