Tags

Type your tag names separated by a space and hit enter

Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels.
Comp Biochem Physiol C Toxicol Pharmacol. 2006 Jul; 143(3):263-74.CB

Abstract

Three populations of brown trout (Salmo trutta) exposed to different metal levels in their natural environments, were studied with respect to antioxidants metallothionein (MT), superoxide dismutase (SOD) and catalase (CAT) as well as for corresponding mRNA levels. In addition, mRNA levels were studied for glutathione peroxidase (GPx) and glutathione reductase (GR). The Cd/Zn-exposed trout (Naustebekken River) had higher accumulated levels of Cd, Cu and Zn in gills, and higher levels of MT (both protein and mRNA) in liver and kidney as well as in gills compared to the Cu-exposed trout (Rugla River) and trout from an uncontaminated reference river (Stribekken River). Less MT found in the Cu-exposed trout may increase susceptibility to oxidative stress, but no higher levels of antioxidant mRNAs were found in gills of these trouts. The data indicated that chronic exposures of brown trout to Cd, Zn and/or Cu did not involve maintenance of high activities of SOD and CAT enzymes in gills, although SOD mRNA levels were higher in the Cd/Zn-exposed trout. In livers, mRNA levels of SOD, CAT and GPx were higher in the metal-exposed trout, but in the case of GR this was only seen in kidneys of Cd/Zn-exposed trout. However, both metal-exposed groups had higher activities of SOD enzyme in liver compared to the unexposed reference trout, and CAT activity was found to be higher in kidneys of Cu-exposed trout. The Cu-exposed trout did not seem to rely on MT production to avoid Cu toxicity in gills, but rather by keeping the Cu uptake at a low level. A coordinated expression of different stress genes may also be important in chronic metal exposure. It may be concluded that the observed metal effects relies on acclimation rather than on genetic adaptation in the metal exposed populations.

Authors+Show Affiliations

Norwegian University of Science and Technology (NTNU), Department of Biology, Høgskoleringen 5, N-7491 Trondheim, Norway. bjorn.henrik.hansen@bio.ntnu.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16616685

Citation

Hansen, B H., et al. "Antioxidative Stress Proteins and Their Gene Expression in Brown Trout (Salmo Trutta) From Three Rivers With Different Heavy Metal Levels." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 143, no. 3, 2006, pp. 263-74.
Hansen BH, Rømma S, Garmo ØA, et al. Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels. Comp Biochem Physiol C Toxicol Pharmacol. 2006;143(3):263-74.
Hansen, B. H., Rømma, S., Garmo, Ø. A., Olsvik, P. A., & Andersen, R. A. (2006). Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 143(3), 263-74.
Hansen BH, et al. Antioxidative Stress Proteins and Their Gene Expression in Brown Trout (Salmo Trutta) From Three Rivers With Different Heavy Metal Levels. Comp Biochem Physiol C Toxicol Pharmacol. 2006;143(3):263-74. PubMed PMID: 16616685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels. AU - Hansen,B H, AU - Rømma,S, AU - Garmo,Ø A, AU - Olsvik,P A, AU - Andersen,R A, Y1 - 2006/03/02/ PY - 2005/11/04/received PY - 2006/02/21/revised PY - 2006/02/22/accepted PY - 2006/4/18/pubmed PY - 2006/9/30/medline PY - 2006/4/18/entrez SP - 263 EP - 74 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 143 IS - 3 N2 - Three populations of brown trout (Salmo trutta) exposed to different metal levels in their natural environments, were studied with respect to antioxidants metallothionein (MT), superoxide dismutase (SOD) and catalase (CAT) as well as for corresponding mRNA levels. In addition, mRNA levels were studied for glutathione peroxidase (GPx) and glutathione reductase (GR). The Cd/Zn-exposed trout (Naustebekken River) had higher accumulated levels of Cd, Cu and Zn in gills, and higher levels of MT (both protein and mRNA) in liver and kidney as well as in gills compared to the Cu-exposed trout (Rugla River) and trout from an uncontaminated reference river (Stribekken River). Less MT found in the Cu-exposed trout may increase susceptibility to oxidative stress, but no higher levels of antioxidant mRNAs were found in gills of these trouts. The data indicated that chronic exposures of brown trout to Cd, Zn and/or Cu did not involve maintenance of high activities of SOD and CAT enzymes in gills, although SOD mRNA levels were higher in the Cd/Zn-exposed trout. In livers, mRNA levels of SOD, CAT and GPx were higher in the metal-exposed trout, but in the case of GR this was only seen in kidneys of Cd/Zn-exposed trout. However, both metal-exposed groups had higher activities of SOD enzyme in liver compared to the unexposed reference trout, and CAT activity was found to be higher in kidneys of Cu-exposed trout. The Cu-exposed trout did not seem to rely on MT production to avoid Cu toxicity in gills, but rather by keeping the Cu uptake at a low level. A coordinated expression of different stress genes may also be important in chronic metal exposure. It may be concluded that the observed metal effects relies on acclimation rather than on genetic adaptation in the metal exposed populations. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/16616685/Antioxidative_stress_proteins_and_their_gene_expression_in_brown_trout__Salmo_trutta__from_three_rivers_with_different_heavy_metal_levels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(06)00056-1 DB - PRIME DP - Unbound Medicine ER -