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Eicosapentaenoic acid and docosahexaenoic acid modulate mitogen-activated protein kinase activity in endothelium.
Vascul Pharmacol. 2006 Jun; 44(6):434-9.VP

Abstract

Omega-3 polyunsaturated fatty acids (PUFA) regulate inflammation and immunoreaction partially via affecting endothelial functions. However, the intracellular signaling mechanisms for inhibiting endothelial activation by omega-3 PUFA remain unclear. We investigated the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on mitogen-activated protein kinases (MAPK) of endothelium. We analyzed the expression of extracellular signal-related kinases (ERK1/2), Jun amino-terminal kinases (JNK), and p38 mRNA by real-time RT-PCR and the kinases activity by western blotting in tumor necrosis factor-alpha (TNF-alpha)-activated human umbilical vein endothelial cells (HUVEC). We observed that EPA or DHA alone significantly reduced the TNF-alpha-induced activation of p38 and JNK kinases at a concentration of 20 microM, but EPA is a more potent inhibitor than DHA. In contrast, both EPA and DHA significantly counteracted the TNF-alpha-mediated deactivation of ERK1/2 kinases. Meanwhile, both EPA and DHA significantly attenuated the TNF-alpha-induced expression of p38 and ERK1/2 mRNA, and DHA but not EPA also reduced the TNF-alpha-induced JNK mRNA expression. We present data show that both EPA and DHA alone diminish activation of p38 and JNK kinases, while maintaining the activation of ERK1/2 kinases of TNF-alpha-stimulated HUVEC. This may contribute to the inhibiting effects of omega-3 PUFA on endothelial activation by proinflammatory stimuli.

Authors+Show Affiliations

Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, No. 305, East Zhongshan Road, Nanjing 210002, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16616699

Citation

Xue, Hua, et al. "Eicosapentaenoic Acid and Docosahexaenoic Acid Modulate Mitogen-activated Protein Kinase Activity in Endothelium." Vascular Pharmacology, vol. 44, no. 6, 2006, pp. 434-9.
Xue H, Wan M, Song D, et al. Eicosapentaenoic acid and docosahexaenoic acid modulate mitogen-activated protein kinase activity in endothelium. Vascul Pharmacol. 2006;44(6):434-9.
Xue, H., Wan, M., Song, D., Li, Y., & Li, J. (2006). Eicosapentaenoic acid and docosahexaenoic acid modulate mitogen-activated protein kinase activity in endothelium. Vascular Pharmacology, 44(6), 434-9.
Xue H, et al. Eicosapentaenoic Acid and Docosahexaenoic Acid Modulate Mitogen-activated Protein Kinase Activity in Endothelium. Vascul Pharmacol. 2006;44(6):434-9. PubMed PMID: 16616699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eicosapentaenoic acid and docosahexaenoic acid modulate mitogen-activated protein kinase activity in endothelium. AU - Xue,Hua, AU - Wan,Meifang, AU - Song,Desheng, AU - Li,Yousheng, AU - Li,Jieshou, Y1 - 2006/04/17/ PY - 2005/10/09/received PY - 2006/02/15/revised PY - 2006/02/27/accepted PY - 2006/4/18/pubmed PY - 2006/8/30/medline PY - 2006/4/18/entrez SP - 434 EP - 9 JF - Vascular pharmacology JO - Vascul Pharmacol VL - 44 IS - 6 N2 - Omega-3 polyunsaturated fatty acids (PUFA) regulate inflammation and immunoreaction partially via affecting endothelial functions. However, the intracellular signaling mechanisms for inhibiting endothelial activation by omega-3 PUFA remain unclear. We investigated the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on mitogen-activated protein kinases (MAPK) of endothelium. We analyzed the expression of extracellular signal-related kinases (ERK1/2), Jun amino-terminal kinases (JNK), and p38 mRNA by real-time RT-PCR and the kinases activity by western blotting in tumor necrosis factor-alpha (TNF-alpha)-activated human umbilical vein endothelial cells (HUVEC). We observed that EPA or DHA alone significantly reduced the TNF-alpha-induced activation of p38 and JNK kinases at a concentration of 20 microM, but EPA is a more potent inhibitor than DHA. In contrast, both EPA and DHA significantly counteracted the TNF-alpha-mediated deactivation of ERK1/2 kinases. Meanwhile, both EPA and DHA significantly attenuated the TNF-alpha-induced expression of p38 and ERK1/2 mRNA, and DHA but not EPA also reduced the TNF-alpha-induced JNK mRNA expression. We present data show that both EPA and DHA alone diminish activation of p38 and JNK kinases, while maintaining the activation of ERK1/2 kinases of TNF-alpha-stimulated HUVEC. This may contribute to the inhibiting effects of omega-3 PUFA on endothelial activation by proinflammatory stimuli. SN - 1537-1891 UR - https://www.unboundmedicine.com/medline/citation/16616699/Eicosapentaenoic_acid_and_docosahexaenoic_acid_modulate_mitogen_activated_protein_kinase_activity_in_endothelium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1537-1891(06)00053-X DB - PRIME DP - Unbound Medicine ER -