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Determination of amiodarone and desethylamiodarone in horse plasma and urine by high-performance liquid chromatography combined with UV detection and electrospray ionization mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 May 19; 836(1-2):47-56.JC

Abstract

A rapid method for the quantification of amiodarone and desethylamiodarone in animal plasma using high-performance liquid chromatography combined with UV detection (HPLC-UV) is presented. The sample preparation includes a simple deproteinisation step with acetonitrile. In addition, a sensitive method for the quantification of amiodarone and desethylamiodarone in horse plasma and urine using high-performance liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is described. The sample preparation includes a solid-phase extraction (SPE) with a SCX column. Tamoxifen is used as an internal standard for both chromatographic methods. Chromatographic separation is achieved on an ODS Hypersil column using isocratic elution with 0.01% diethylamine and acetonitrile as mobile phase for the HPLC-UV method and with 0.1% formic acid and acetonitrile as mobile phase for the LC-MS/MS method. For the HPLC-UV method, good linearity was observed in the range 0-5 microg ml(-1), and in the range 0-1 microg ml(-1) for the LC-MS/MS method. The limit of quantification (LOQ) was set at 50 and 5 ng ml(-1) for the HPLC-UV method and the LC-MS/MS method, respectively. For the UV method, the limit of detection (LOD) was 15 and 10 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The LODs of the LC-MS/MS method in plasma were much lower, i.e. 0.10 and 0.04 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The LODs obtained for the urine samples were 0.16 and 0.09 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The methods were shown to be of use in horses. The rapid HPLC-UV method was used for therapeutic drug monitoring after amiodarone treatment, while the LC-MS/MS method showed its applicability for single dose pharmacokinetic studies.

Authors+Show Affiliations

Department of Pharmacology, Toxicology, Biochemistry and Organ Physiology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. an.maes@ugent.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

16616880

Citation

Maes, A, et al. "Determination of Amiodarone and Desethylamiodarone in Horse Plasma and Urine By High-performance Liquid Chromatography Combined With UV Detection and Electrospray Ionization Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 836, no. 1-2, 2006, pp. 47-56.
Maes A, Baert K, Croubels S, et al. Determination of amiodarone and desethylamiodarone in horse plasma and urine by high-performance liquid chromatography combined with UV detection and electrospray ionization mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;836(1-2):47-56.
Maes, A., Baert, K., Croubels, S., De Clercq, D., van Loon, G., Deprez, P., & De Backer, P. (2006). Determination of amiodarone and desethylamiodarone in horse plasma and urine by high-performance liquid chromatography combined with UV detection and electrospray ionization mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 836(1-2), 47-56.
Maes A, et al. Determination of Amiodarone and Desethylamiodarone in Horse Plasma and Urine By High-performance Liquid Chromatography Combined With UV Detection and Electrospray Ionization Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 May 19;836(1-2):47-56. PubMed PMID: 16616880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amiodarone and desethylamiodarone in horse plasma and urine by high-performance liquid chromatography combined with UV detection and electrospray ionization mass spectrometry. AU - Maes,A, AU - Baert,K, AU - Croubels,S, AU - De Clercq,D, AU - van Loon,G, AU - Deprez,P, AU - De Backer,P, Y1 - 2006/04/17/ PY - 2005/11/16/received PY - 2006/02/15/revised PY - 2006/03/14/accepted PY - 2006/4/18/pubmed PY - 2006/8/15/medline PY - 2006/4/18/entrez SP - 47 EP - 56 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 836 IS - 1-2 N2 - A rapid method for the quantification of amiodarone and desethylamiodarone in animal plasma using high-performance liquid chromatography combined with UV detection (HPLC-UV) is presented. The sample preparation includes a simple deproteinisation step with acetonitrile. In addition, a sensitive method for the quantification of amiodarone and desethylamiodarone in horse plasma and urine using high-performance liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is described. The sample preparation includes a solid-phase extraction (SPE) with a SCX column. Tamoxifen is used as an internal standard for both chromatographic methods. Chromatographic separation is achieved on an ODS Hypersil column using isocratic elution with 0.01% diethylamine and acetonitrile as mobile phase for the HPLC-UV method and with 0.1% formic acid and acetonitrile as mobile phase for the LC-MS/MS method. For the HPLC-UV method, good linearity was observed in the range 0-5 microg ml(-1), and in the range 0-1 microg ml(-1) for the LC-MS/MS method. The limit of quantification (LOQ) was set at 50 and 5 ng ml(-1) for the HPLC-UV method and the LC-MS/MS method, respectively. For the UV method, the limit of detection (LOD) was 15 and 10 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The LODs of the LC-MS/MS method in plasma were much lower, i.e. 0.10 and 0.04 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The LODs obtained for the urine samples were 0.16 and 0.09 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The methods were shown to be of use in horses. The rapid HPLC-UV method was used for therapeutic drug monitoring after amiodarone treatment, while the LC-MS/MS method showed its applicability for single dose pharmacokinetic studies. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16616880/Determination_of_amiodarone_and_desethylamiodarone_in_horse_plasma_and_urine_by_high_performance_liquid_chromatography_combined_with_UV_detection_and_electrospray_ionization_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)00252-2 DB - PRIME DP - Unbound Medicine ER -