Tags

Type your tag names separated by a space and hit enter

[Diagnostic validity of anti-tissue transglutaminase and anti-endomysium antibodies in children with celiac disease].
Pol Merkur Lekarski 2006; 20(115):17-21PM

Abstract

Tissue transglutaminase (tTG) plays a decisive role in the pathomechanism of celiac disease. Unchanged gliadin peptides bind to HLA-DQ2 with a week affinity, after that they are recognized by gliadin specific T-lymphocytes and the whole immune reaction is started which damages intestine mucous and causes the release of tTG. Tissue transglutaminase deaminates gliadin peptides enhancing the whole process. The aim of this study was determination of correlation between antiendomysial antibodies and anti-tissue transglutaminase antibodies in children with celiac disease, and the assessment of their usefulness in the diagnosis of celiac disease.

MATERIAL AND METHODS

109 children, aged 6 moths to 16 years (average 8.8 years) were included into the study. In all children an intestine biopsy was performed and/or antiendomysial antibodies were determined. In all children tissue transglutaminase antibodies were measured. Antibodies against tissue transglutaminase were determined using ELISA method. Antiendomysial antibodies were determined using the immunofluorescence method.

RESULTS

In patients with normal mucous with lack of lymphocyte infiltration no IgAEmA were present in the blood. In the groups with subtotal and total villous atrophy all IgAEmA titres were positive. A positive titre of IgAEmA was observed only in childrerl with villous atrophy, which yields specificity of the test of 100%, sensitivity was 81%. Sensitivity of the anti-tTG antibodies test for detecting villous atrophy was 83% for IgA and 52% for IgG. There is a positive correlation between IgAEmA and anti-tTG antibodies.

CONCLUSIONS

High sensitivity and specificity of determination of antibodies against tTG in IgA class causes that these tests may serve as a screening test in diagnosis and monitoring of celiac disease in children.

Authors+Show Affiliations

Matusiewicz K
Akademia Medyczna we Wroclawiu, II Katedra i Klinika Pediatrii, Gastroenterologii i Zywienia.
Iwańczak B
No affiliation info available
Matusiewicz M
No affiliation info available
Iwańczak F
No affiliation info available

MeSH

AdolescentAntibodies, Anti-IdiotypicBiopsyCeliac DiseaseChildChild, PreschoolEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody TechniqueHLA-DQ AntigensHumansImmunoglobulin AInfantIntestinal MucosaMaleMuscle Fibers, SkeletalPredictive Value of TestsReproducibility of ResultsTransglutaminases

Pub Type(s)

Journal Article
Validation Study

Language

pol

PubMed ID

16617728

Citation

Matusiewicz, Krzysztof, et al. "[Diagnostic Validity of Anti-tissue Transglutaminase and Anti-endomysium Antibodies in Children With Celiac Disease]." Polski Merkuriusz Lekarski : Organ Polskiego Towarzystwa Lekarskiego, vol. 20, no. 115, 2006, pp. 17-21.
Matusiewicz K, Iwańczak B, Matusiewicz M, et al. [Diagnostic validity of anti-tissue transglutaminase and anti-endomysium antibodies in children with celiac disease]. Pol Merkur Lekarski. 2006;20(115):17-21.
Matusiewicz, K., Iwańczak, B., Matusiewicz, M., & Iwańczak, F. (2006). [Diagnostic validity of anti-tissue transglutaminase and anti-endomysium antibodies in children with celiac disease]. Polski Merkuriusz Lekarski : Organ Polskiego Towarzystwa Lekarskiego, 20(115), pp. 17-21.
Matusiewicz K, et al. [Diagnostic Validity of Anti-tissue Transglutaminase and Anti-endomysium Antibodies in Children With Celiac Disease]. Pol Merkur Lekarski. 2006;20(115):17-21. PubMed PMID: 16617728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Diagnostic validity of anti-tissue transglutaminase and anti-endomysium antibodies in children with celiac disease]. AU - Matusiewicz,Krzysztof, AU - Iwańczak,Barbara, AU - Matusiewicz,Małgorzata, AU - Iwańczak,Franciszek, PY - 2006/4/19/pubmed PY - 2006/7/11/medline PY - 2006/4/19/entrez SP - 17 EP - 21 JF - Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego JO - Pol. Merkur. Lekarski VL - 20 IS - 115 N2 - UNLABELLED: Tissue transglutaminase (tTG) plays a decisive role in the pathomechanism of celiac disease. Unchanged gliadin peptides bind to HLA-DQ2 with a week affinity, after that they are recognized by gliadin specific T-lymphocytes and the whole immune reaction is started which damages intestine mucous and causes the release of tTG. Tissue transglutaminase deaminates gliadin peptides enhancing the whole process. The aim of this study was determination of correlation between antiendomysial antibodies and anti-tissue transglutaminase antibodies in children with celiac disease, and the assessment of their usefulness in the diagnosis of celiac disease. MATERIAL AND METHODS: 109 children, aged 6 moths to 16 years (average 8.8 years) were included into the study. In all children an intestine biopsy was performed and/or antiendomysial antibodies were determined. In all children tissue transglutaminase antibodies were measured. Antibodies against tissue transglutaminase were determined using ELISA method. Antiendomysial antibodies were determined using the immunofluorescence method. RESULTS: In patients with normal mucous with lack of lymphocyte infiltration no IgAEmA were present in the blood. In the groups with subtotal and total villous atrophy all IgAEmA titres were positive. A positive titre of IgAEmA was observed only in childrerl with villous atrophy, which yields specificity of the test of 100%, sensitivity was 81%. Sensitivity of the anti-tTG antibodies test for detecting villous atrophy was 83% for IgA and 52% for IgG. There is a positive correlation between IgAEmA and anti-tTG antibodies. CONCLUSIONS: High sensitivity and specificity of determination of antibodies against tTG in IgA class causes that these tests may serve as a screening test in diagnosis and monitoring of celiac disease in children. SN - 1426-9686 UR - https://www.unboundmedicine.com/medline/citation/16617728/[Diagnostic_validity_of_anti_tissue_transglutaminase_and_anti_endomysium_antibodies_in_children_with_celiac_disease]_ L2 - http://www.diseaseinfosearch.org/result/1186 DB - PRIME DP - Unbound Medicine ER -