Tags

Type your tag names separated by a space and hit enter

Molecular diversity of dinoflagellate symbionts of Cnidaria: the psbA minicircle of Symbiodinium.
Protist. 2006 Jun; 157(2):159-71.P

Abstract

Dinoflagellate algae of the genus Symbiodinium are important symbionts within corals and other benthic marine animals. The molecular diversity of Symbiodinium has been described mainly by use of ribosomal DNA sequence data. We tested whether minicircle sequences, which appear to form the chloroplast genome in many dinoflagellates, could be used as a marker for molecular diversity among symbionts found in corals and sea anemones. Partial and full-length sequences for psbA were obtained from environmental samples of coral and sea anemones of wide-ranging geographical distribution. Phylogenetic trees constructed with partial psbA sequences were consistent with the known phylotypes of the isolates. Further sequencing suggested that the psbA gene is present on a minicircle in all Symbiodinium phylotypes. The length and DNA sequence of the non-coding portion of the minicircles varied considerably among Symbiodinium phylotypes. In two Symbiodinium isolates from different phylotypes an elaborate pattern of repeat sequences of unknown function was found in the non-coding region. Phylogenetic analysis of the non-coding region of the psbA minicircle indicates that minicircle sequences could be a useful chloroplast-derived marker for differentiating both closely related and distantly related Symbiodinium isolates.

Authors+Show Affiliations

Barbrook AC
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK. acb18@mole.bio.cam.ac.uk
Visram S
No affiliation info available
Douglas AE
No affiliation info available
Howe CJ
No affiliation info available

MeSH

Amino Acid SequenceAnimalsBase SequenceCnidariaDNA, KinetoplastDNA, ProtozoanDNA, RibosomalDinoflagellidaMolecular Sequence DataPhotosystem II Protein ComplexPhylogenyPolymerase Chain ReactionProtozoan ProteinsSeawaterSequence AlignmentSymbiosis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16618551

Citation

Barbrook, Adrian C., et al. "Molecular Diversity of Dinoflagellate Symbionts of Cnidaria: the psbA Minicircle of Symbiodinium." Protist, vol. 157, no. 2, 2006, pp. 159-71.
Barbrook AC, Visram S, Douglas AE, et al. Molecular diversity of dinoflagellate symbionts of Cnidaria: the psbA minicircle of Symbiodinium. Protist. 2006;157(2):159-71.
Barbrook, A. C., Visram, S., Douglas, A. E., & Howe, C. J. (2006). Molecular diversity of dinoflagellate symbionts of Cnidaria: the psbA minicircle of Symbiodinium. Protist, 157(2), 159-71.
Barbrook AC, et al. Molecular Diversity of Dinoflagellate Symbionts of Cnidaria: the psbA Minicircle of Symbiodinium. Protist. 2006;157(2):159-71. PubMed PMID: 16618551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular diversity of dinoflagellate symbionts of Cnidaria: the psbA minicircle of Symbiodinium. AU - Barbrook,Adrian C, AU - Visram,Shakil, AU - Douglas,Angela E, AU - Howe,Christopher J, Y1 - 2006/04/17/ PY - 2005/07/29/received PY - 2005/12/27/accepted PY - 2006/4/19/pubmed PY - 2006/8/31/medline PY - 2006/4/19/entrez SP - 159 EP - 71 JF - Protist JO - Protist VL - 157 IS - 2 N2 - Dinoflagellate algae of the genus Symbiodinium are important symbionts within corals and other benthic marine animals. The molecular diversity of Symbiodinium has been described mainly by use of ribosomal DNA sequence data. We tested whether minicircle sequences, which appear to form the chloroplast genome in many dinoflagellates, could be used as a marker for molecular diversity among symbionts found in corals and sea anemones. Partial and full-length sequences for psbA were obtained from environmental samples of coral and sea anemones of wide-ranging geographical distribution. Phylogenetic trees constructed with partial psbA sequences were consistent with the known phylotypes of the isolates. Further sequencing suggested that the psbA gene is present on a minicircle in all Symbiodinium phylotypes. The length and DNA sequence of the non-coding portion of the minicircles varied considerably among Symbiodinium phylotypes. In two Symbiodinium isolates from different phylotypes an elaborate pattern of repeat sequences of unknown function was found in the non-coding region. Phylogenetic analysis of the non-coding region of the psbA minicircle indicates that minicircle sequences could be a useful chloroplast-derived marker for differentiating both closely related and distantly related Symbiodinium isolates. SN - 1434-4610 UR - https://www.unboundmedicine.com/medline/citation/16618551/Molecular_diversity_of_dinoflagellate_symbionts_of_Cnidaria:_the_psbA_minicircle_of_Symbiodinium_ DB - PRIME DP - Unbound Medicine ER -