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Cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase in the guinea pig inner ear. Evaluation of the cerium-based method vs. the lead-based method.
Acta Otolaryngol. 1991; 111(5):885-90.AO

Abstract

The cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase in the guinea pig inner ear was studied using both the lead-based method and the cerium-based method. The influence of primary fixation and incubation time was investigated under both incubation conditions. A high p-NPPase activity was observed in the basolateral infoldings of the stria vascularis in the cochlea and the dark cells in the vestibular labyrinth. The stromal cells of the spiral prominence only showed a weak reaction with the lead-based method. No specific enzyme activity was found in the endolymphatic sac. The results of the present study are discussed with emphasis on the cation transport mechanisms of the inner ear.

Authors+Show Affiliations

Department of Otorhinolaryngology, University Hospital Utrecht, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1662003

Citation

Albers, F W., et al. "Cytochemical Localization of Ouabain-sensitive, Potassium-dependent P-nitrophenylphosphatase in the Guinea Pig Inner Ear. Evaluation of the Cerium-based Method Vs. the Lead-based Method." Acta Oto-laryngologica, vol. 111, no. 5, 1991, pp. 885-90.
Albers FW, Van Benthem PP, De Groot JC. Cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase in the guinea pig inner ear. Evaluation of the cerium-based method vs. the lead-based method. Acta Otolaryngol. 1991;111(5):885-90.
Albers, F. W., Van Benthem, P. P., & De Groot, J. C. (1991). Cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase in the guinea pig inner ear. Evaluation of the cerium-based method vs. the lead-based method. Acta Oto-laryngologica, 111(5), 885-90.
Albers FW, Van Benthem PP, De Groot JC. Cytochemical Localization of Ouabain-sensitive, Potassium-dependent P-nitrophenylphosphatase in the Guinea Pig Inner Ear. Evaluation of the Cerium-based Method Vs. the Lead-based Method. Acta Otolaryngol. 1991;111(5):885-90. PubMed PMID: 1662003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase in the guinea pig inner ear. Evaluation of the cerium-based method vs. the lead-based method. AU - Albers,F W, AU - Van Benthem,P P, AU - De Groot,J C, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 885 EP - 90 JF - Acta oto-laryngologica JO - Acta Otolaryngol VL - 111 IS - 5 N2 - The cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase in the guinea pig inner ear was studied using both the lead-based method and the cerium-based method. The influence of primary fixation and incubation time was investigated under both incubation conditions. A high p-NPPase activity was observed in the basolateral infoldings of the stria vascularis in the cochlea and the dark cells in the vestibular labyrinth. The stromal cells of the spiral prominence only showed a weak reaction with the lead-based method. No specific enzyme activity was found in the endolymphatic sac. The results of the present study are discussed with emphasis on the cation transport mechanisms of the inner ear. SN - 0001-6489 UR - https://www.unboundmedicine.com/medline/citation/1662003/Cytochemical_localization_of_ouabain_sensitive_potassium_dependent_p_nitrophenylphosphatase_in_the_guinea_pig_inner_ear__Evaluation_of_the_cerium_based_method_vs__the_lead_based_method_ L2 - https://www.tandfonline.com/doi/full/10.3109/00016489109138426 DB - PRIME DP - Unbound Medicine ER -