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Application of high-speed counter-current chromatography coupled with high-performance liquid chromatography-diode array detection for the preparative isolation and purification of hyperoside from Hypericum perforatum with online purity monitoring.
J Chromatogr A. 2006 May 26; 1116(1-2):97-101.JC

Abstract

Following preparative isolation and purification by high-speed counter-current chromatography (HSCCC), the collected fractions were generally analyzed by high-performance liquid chromatography (HPLC) to determine the relative purities of each fraction. Our paper reports for the first time a preparative isolation-purity detection hyphenated system: online coupling of HSCCC with high-performance liquid chromatography-diode array detection (HSCCC-HPLC-DAD). The introduction of online purity analysis in HSCCC has dramatically improved the efficiency of this technique by overcoming the drawbacks of post analysis in HSCCC isolation. The effluent from the outlet of HSCCC was splitted into two parts: one was collected, while the other was introduced directly into an HPLC-DAD system for purity analysis through a switch valve. Therefore, the purities of the obtained fractions from HSCCC were monitored, and fractions with high purities were collected. This strategy has been successfully demonstrated with the preparative isolation and purification of hyperoside from Hypericum perforatum (St. Jone's Wort); a model of TBE-300A HSCCC was used to isolate and separate hyperoside from H. perforatum with a two-phase solvent system composed of ethyl acetate-ethanol-water at the volume ratio of 5:1:5 (v/v) using online detection technique. The isolation was done in less than 3.5 h, and a total of 83.0-mg hyperoside at over 99.0% purity was yielded from 300 mg of the partially purified extract. This new strategy possesses general utility in the preparation of bioactive compounds from traditional Chinese medicine (TCM).

Authors+Show Affiliations

Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16620843

Citation

Zhou, Tingting, et al. "Application of High-speed Counter-current Chromatography Coupled With High-performance Liquid Chromatography-diode Array Detection for the Preparative Isolation and Purification of Hyperoside From Hypericum Perforatum With Online Purity Monitoring." Journal of Chromatography. A, vol. 1116, no. 1-2, 2006, pp. 97-101.
Zhou T, Chen B, Fan G, et al. Application of high-speed counter-current chromatography coupled with high-performance liquid chromatography-diode array detection for the preparative isolation and purification of hyperoside from Hypericum perforatum with online purity monitoring. J Chromatogr A. 2006;1116(1-2):97-101.
Zhou, T., Chen, B., Fan, G., Chai, Y., & Wu, Y. (2006). Application of high-speed counter-current chromatography coupled with high-performance liquid chromatography-diode array detection for the preparative isolation and purification of hyperoside from Hypericum perforatum with online purity monitoring. Journal of Chromatography. A, 1116(1-2), 97-101.
Zhou T, et al. Application of High-speed Counter-current Chromatography Coupled With High-performance Liquid Chromatography-diode Array Detection for the Preparative Isolation and Purification of Hyperoside From Hypericum Perforatum With Online Purity Monitoring. J Chromatogr A. 2006 May 26;1116(1-2):97-101. PubMed PMID: 16620843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of high-speed counter-current chromatography coupled with high-performance liquid chromatography-diode array detection for the preparative isolation and purification of hyperoside from Hypericum perforatum with online purity monitoring. AU - Zhou,Tingting, AU - Chen,Bin, AU - Fan,Guorong, AU - Chai,Yifeng, AU - Wu,Yutian, Y1 - 2006/04/18/ PY - 2006/01/17/received PY - 2006/03/01/revised PY - 2006/03/08/accepted PY - 2006/4/20/pubmed PY - 2006/7/6/medline PY - 2006/4/20/entrez SP - 97 EP - 101 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1116 IS - 1-2 N2 - Following preparative isolation and purification by high-speed counter-current chromatography (HSCCC), the collected fractions were generally analyzed by high-performance liquid chromatography (HPLC) to determine the relative purities of each fraction. Our paper reports for the first time a preparative isolation-purity detection hyphenated system: online coupling of HSCCC with high-performance liquid chromatography-diode array detection (HSCCC-HPLC-DAD). The introduction of online purity analysis in HSCCC has dramatically improved the efficiency of this technique by overcoming the drawbacks of post analysis in HSCCC isolation. The effluent from the outlet of HSCCC was splitted into two parts: one was collected, while the other was introduced directly into an HPLC-DAD system for purity analysis through a switch valve. Therefore, the purities of the obtained fractions from HSCCC were monitored, and fractions with high purities were collected. This strategy has been successfully demonstrated with the preparative isolation and purification of hyperoside from Hypericum perforatum (St. Jone's Wort); a model of TBE-300A HSCCC was used to isolate and separate hyperoside from H. perforatum with a two-phase solvent system composed of ethyl acetate-ethanol-water at the volume ratio of 5:1:5 (v/v) using online detection technique. The isolation was done in less than 3.5 h, and a total of 83.0-mg hyperoside at over 99.0% purity was yielded from 300 mg of the partially purified extract. This new strategy possesses general utility in the preparation of bioactive compounds from traditional Chinese medicine (TCM). SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16620843/Application_of_high_speed_counter_current_chromatography_coupled_with_high_performance_liquid_chromatography_diode_array_detection_for_the_preparative_isolation_and_purification_of_hyperoside_from_Hypericum_perforatum_with_online_purity_monitoring_ DB - PRIME DP - Unbound Medicine ER -