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Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection.
J Chromatogr A. 2006 Oct 20; 1130(2):259-64.JC

Abstract

A simple method for separation of different anionic and zwitterionic phospholipid classes by capillary zone electrophoresis (CZE), using indirect UV detection with adenosine monophosphate (AMP) as background electrolyte and the UV-absorbing additive, was successfully developed in this study. The separation conditions including apparent pH (pH*) of running buffer, concentration of AMP, organic solvent, applied voltage and capillary temperature were systematically optimized. The application of this method to human blood sample was also briefly examined.

Authors+Show Affiliations

Gao F
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemistry, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Dong J
No affiliation info available
Li W
No affiliation info available
Wang T
No affiliation info available
Liao J
No affiliation info available
Liao Y
No affiliation info available
Liu H
No affiliation info available

MeSH

Adenosine MonophosphateElectrophoresis, CapillaryHumansHydrogen-Ion ConcentrationPhospholipidsReproducibility of ResultsSolventsSpectrophotometry, UltravioletTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16620855

Citation

Gao, Fei, et al. "Separation of Phospholipids By Capillary Zone Electrophoresis With Indirect Ultraviolet Detection." Journal of Chromatography. A, vol. 1130, no. 2, 2006, pp. 259-64.
Gao F, Dong J, Li W, et al. Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection. J Chromatogr A. 2006;1130(2):259-64.
Gao, F., Dong, J., Li, W., Wang, T., Liao, J., Liao, Y., & Liu, H. (2006). Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection. Journal of Chromatography. A, 1130(2), 259-64.
Gao F, et al. Separation of Phospholipids By Capillary Zone Electrophoresis With Indirect Ultraviolet Detection. J Chromatogr A. 2006 Oct 20;1130(2):259-64. PubMed PMID: 16620855.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection. AU - Gao,Fei, AU - Dong,Juan, AU - Li,Wei, AU - Wang,Tao, AU - Liao,Jie, AU - Liao,Yiping, AU - Liu,Huwei, Y1 - 2006/04/18/ PY - 2006/01/07/received PY - 2006/03/05/revised PY - 2006/03/21/accepted PY - 2006/4/20/pubmed PY - 2006/12/9/medline PY - 2006/4/20/entrez SP - 259 EP - 64 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1130 IS - 2 N2 - A simple method for separation of different anionic and zwitterionic phospholipid classes by capillary zone electrophoresis (CZE), using indirect UV detection with adenosine monophosphate (AMP) as background electrolyte and the UV-absorbing additive, was successfully developed in this study. The separation conditions including apparent pH (pH*) of running buffer, concentration of AMP, organic solvent, applied voltage and capillary temperature were systematically optimized. The application of this method to human blood sample was also briefly examined. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16620855/Separation_of_phospholipids_by_capillary_zone_electrophoresis_with_indirect_ultraviolet_detection_ DB - PRIME DP - Unbound Medicine ER -