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In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae.
J Biotechnol. 2006 Sep 18; 125(3):377-84.JB

Abstract

Dilute-acid lignocellulosic hydrolyzate was successfully fermented to ethanol by encapsulated Saccharomyces cerevisiae at dilution rates up to 0.5h(-1). The hydrolyzate was so toxic that freely suspended yeast cells could ferment it continuously just up to dilution rate 0.1h(-1), where the cells lost 75% of their viability measured by colony forming unit (CFU). However, encapsulation increased their capacity for in situ detoxification of the hydrolyzate and protected the cells against the inhibitors present in the hydrolyzate. While the cells were encapsulated, they could successfully ferment the hydrolyzate at tested dilution rates 0.1-0.5h(-1), and keep more than 75% cell viability in the worst conditions. They produced ethanol with yield 0.44+/-0.01 g/g and specific productivity 0.14-0.17 g/(gh) at all dilution rates. Glycerol was the main by-product of the cultivations, which yielded 0.039-0.052 g/g. HMF present in the hydrolyzate was converted 48-71% by the encapsulated yeast, while furfural was totally converted at dilution rates 0.1 and 0.2h(-1) and partly at the higher rates. Continuous cultivation of encapsulated yeast was also investigated on glucose in synthetic medium up to dilution rate 1.0 h(-1). At this highest rate, ethanol and glycerol were also the major products with yields 0.43 and 0.076 g/g, respectively. The experiments lasted for 18-21 days, and no damage in the capsules was detected.

Authors+Show Affiliations

School of Engineering, University College of Borås, 501 90 Borås, Sweden. farid.talebnia@hb.se <farid.talebnia@hb.se>No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16621080

Citation

Talebnia, Farid, and Mohammad J. Taherzadeh. "In Situ Detoxification and Continuous Cultivation of Dilute-acid Hydrolyzate to Ethanol By Encapsulated S. Cerevisiae." Journal of Biotechnology, vol. 125, no. 3, 2006, pp. 377-84.
Talebnia F, Taherzadeh MJ. In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae. J Biotechnol. 2006;125(3):377-84.
Talebnia, F., & Taherzadeh, M. J. (2006). In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae. Journal of Biotechnology, 125(3), 377-84.
Talebnia F, Taherzadeh MJ. In Situ Detoxification and Continuous Cultivation of Dilute-acid Hydrolyzate to Ethanol By Encapsulated S. Cerevisiae. J Biotechnol. 2006 Sep 18;125(3):377-84. PubMed PMID: 16621080.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae. AU - Talebnia,Farid, AU - Taherzadeh,Mohammad J, Y1 - 2006/04/18/ PY - 2005/09/07/received PY - 2006/02/28/revised PY - 2006/03/13/accepted PY - 2006/4/20/pubmed PY - 2006/12/23/medline PY - 2006/4/20/entrez SP - 377 EP - 84 JF - Journal of biotechnology JO - J Biotechnol VL - 125 IS - 3 N2 - Dilute-acid lignocellulosic hydrolyzate was successfully fermented to ethanol by encapsulated Saccharomyces cerevisiae at dilution rates up to 0.5h(-1). The hydrolyzate was so toxic that freely suspended yeast cells could ferment it continuously just up to dilution rate 0.1h(-1), where the cells lost 75% of their viability measured by colony forming unit (CFU). However, encapsulation increased their capacity for in situ detoxification of the hydrolyzate and protected the cells against the inhibitors present in the hydrolyzate. While the cells were encapsulated, they could successfully ferment the hydrolyzate at tested dilution rates 0.1-0.5h(-1), and keep more than 75% cell viability in the worst conditions. They produced ethanol with yield 0.44+/-0.01 g/g and specific productivity 0.14-0.17 g/(gh) at all dilution rates. Glycerol was the main by-product of the cultivations, which yielded 0.039-0.052 g/g. HMF present in the hydrolyzate was converted 48-71% by the encapsulated yeast, while furfural was totally converted at dilution rates 0.1 and 0.2h(-1) and partly at the higher rates. Continuous cultivation of encapsulated yeast was also investigated on glucose in synthetic medium up to dilution rate 1.0 h(-1). At this highest rate, ethanol and glycerol were also the major products with yields 0.43 and 0.076 g/g, respectively. The experiments lasted for 18-21 days, and no damage in the capsules was detected. SN - 0168-1656 UR - https://www.unboundmedicine.com/medline/citation/16621080/In_situ_detoxification_and_continuous_cultivation_of_dilute_acid_hydrolyzate_to_ethanol_by_encapsulated_S__cerevisiae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(06)00210-0 DB - PRIME DP - Unbound Medicine ER -