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Highly selective and sensitive determination of dopamine using a Nafion/carbon nanotubes coated poly(3-methylthiophene) modified electrode.
Biosens Bioelectron. 2006 Dec 15; 22(5):664-9.BB

Abstract

A poly(3-methylthiophene) modified glassy carbon electrode coated with Nafion/single-walled carbon nanotubes film was fabricated and used for highly selective and sensitive determination of dopamine. The hybrid film surface of the modified electrode was characterized by scanning electrochemical microscopy (SECM) and the results indicated that the carbon nanotubes were dispersed uniformly on the conductive polymer. The experimental results suggest that the hybrid film modified electrode combining the advantages of poly(3-methylthiophene), carbon nanotubes with Nafion exhibits dramatic electrocatalytic effect on the oxidation of dopamine (DA) and results in a marked enhancement of the current response. In 0.1M phosphate buffer solution (PBS) of pH 7.0, the differential pulse voltammetric (DPV) peak heights are linear with DA concentration in three intervals, viz. 0.020-0.10 microM, 0.10-1.0 microM and 1.0-6.0 microM, with correlation coefficients of 0.9993, 0.9996 and 0.9993, respectively. The detection limit of 5.0 nM DA could be estimated (S/N=3). Moreover, the interferences of ascorbic acid (AA) and uric acid (UC) are effectively diminished. This hybrid film modified electrode can be applied to the determination of DA contents in dopamine hydrochloride injection and human serum. These attractive features provide a potential application for either in vitro measurement of DA in the presence of excess AA and UA or as detectors in flow injection analysis (FIA) and high performance liquid chromatography (HPLC).

Authors+Show Affiliations

Wang HS
College of Chemistry and Chemical Engineering, Liaocheng University, Cultural Road 34, Liaocheng, Shandong 252059, China. hswang@lctu.edu.cn
Li TH
No affiliation info available
Jia WL
No affiliation info available
Xu HY
No affiliation info available

MeSH

Biosensing TechniquesCoated Materials, BiocompatibleDopamineElectrochemistryEquipment DesignEquipment Failure AnalysisFluorocarbon PolymersMicroelectrodesNanotubes, CarbonParticle SizePolymersReproducibility of ResultsSensitivity and SpecificityThiophenes

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16621509

Citation

Wang, Huai-Sheng, et al. "Highly Selective and Sensitive Determination of Dopamine Using a Nafion/carbon Nanotubes Coated Poly(3-methylthiophene) Modified Electrode." Biosensors & Bioelectronics, vol. 22, no. 5, 2006, pp. 664-9.
Wang HS, Li TH, Jia WL, et al. Highly selective and sensitive determination of dopamine using a Nafion/carbon nanotubes coated poly(3-methylthiophene) modified electrode. Biosens Bioelectron. 2006;22(5):664-9.
Wang, H. S., Li, T. H., Jia, W. L., & Xu, H. Y. (2006). Highly selective and sensitive determination of dopamine using a Nafion/carbon nanotubes coated poly(3-methylthiophene) modified electrode. Biosensors & Bioelectronics, 22(5), 664-9.
Wang HS, et al. Highly Selective and Sensitive Determination of Dopamine Using a Nafion/carbon Nanotubes Coated Poly(3-methylthiophene) Modified Electrode. Biosens Bioelectron. 2006 Dec 15;22(5):664-9. PubMed PMID: 16621509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly selective and sensitive determination of dopamine using a Nafion/carbon nanotubes coated poly(3-methylthiophene) modified electrode. AU - Wang,Huai-Sheng, AU - Li,Tian-Hua, AU - Jia,Wen-Li, AU - Xu,Hong-Yan, Y1 - 2006/04/18/ PY - 2005/10/07/received PY - 2005/12/12/revised PY - 2006/02/06/accepted PY - 2006/4/20/pubmed PY - 2007/1/18/medline PY - 2006/4/20/entrez SP - 664 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 5 N2 - A poly(3-methylthiophene) modified glassy carbon electrode coated with Nafion/single-walled carbon nanotubes film was fabricated and used for highly selective and sensitive determination of dopamine. The hybrid film surface of the modified electrode was characterized by scanning electrochemical microscopy (SECM) and the results indicated that the carbon nanotubes were dispersed uniformly on the conductive polymer. The experimental results suggest that the hybrid film modified electrode combining the advantages of poly(3-methylthiophene), carbon nanotubes with Nafion exhibits dramatic electrocatalytic effect on the oxidation of dopamine (DA) and results in a marked enhancement of the current response. In 0.1M phosphate buffer solution (PBS) of pH 7.0, the differential pulse voltammetric (DPV) peak heights are linear with DA concentration in three intervals, viz. 0.020-0.10 microM, 0.10-1.0 microM and 1.0-6.0 microM, with correlation coefficients of 0.9993, 0.9996 and 0.9993, respectively. The detection limit of 5.0 nM DA could be estimated (S/N=3). Moreover, the interferences of ascorbic acid (AA) and uric acid (UC) are effectively diminished. This hybrid film modified electrode can be applied to the determination of DA contents in dopamine hydrochloride injection and human serum. These attractive features provide a potential application for either in vitro measurement of DA in the presence of excess AA and UA or as detectors in flow injection analysis (FIA) and high performance liquid chromatography (HPLC). SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16621509/Highly_selective_and_sensitive_determination_of_dopamine_using_a_Nafion/carbon_nanotubes_coated_poly_3_methylthiophene__modified_electrode_ DB - PRIME DP - Unbound Medicine ER -
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