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Amperometric biosensor based on tyrosinase immobilized on a boron-doped diamond electrode.
Biosens Bioelectron. 2007 Jan 15; 22(6):822-8.BB

Abstract

A novel method has been developed to immobilize tyrosinase onto the surface of boron-doped diamond (BDD) electrode. The hydrogen-terminated BDD (HBDD) surface was first functionalized by photochemically linking vinyl groups of allylamine, producing covalently linked amine-terminated active BDD (ABDD) surface. Then the tyrosinase was immobilized onto the ABDD surface by carbodiimide coupling reaction. The amperometric response was measured as a function of concentration of phenolic compounds in 0.1M phosphate buffer solution (pH 6.5). The tyrosinase-modified ABDD electrode gave a linear response range of 1-175, 1-200 and 1-200 microM and sensitivity of 80.0, 181.4 and 110.0 mA M(-1)cm(-2) for phenol, p-cresol, 4-chlorophenol, respectively. Moreover, selective detection of dopamine (DA) in the presence of ascorbic acid (AA) has been demonstrated with the tyrosinase-modified ABDD electrode. Linearity was observed within the range of 5-120 microM. The above enzyme electrode could maintain 90% of its original activity after intermittent use for 1 month when storing in a dry state at 4 degrees C.

Authors+Show Affiliations

Laboratory of Photoelectro Chemistry, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Zhong Guan Cun, Haidian District, Beijing 100080, People's Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16621510

Citation

Zhou, Yan Li, et al. "Amperometric Biosensor Based On Tyrosinase Immobilized On a Boron-doped Diamond Electrode." Biosensors & Bioelectronics, vol. 22, no. 6, 2007, pp. 822-8.
Zhou YL, Tian RH, Zhi JF. Amperometric biosensor based on tyrosinase immobilized on a boron-doped diamond electrode. Biosens Bioelectron. 2007;22(6):822-8.
Zhou, Y. L., Tian, R. H., & Zhi, J. F. (2007). Amperometric biosensor based on tyrosinase immobilized on a boron-doped diamond electrode. Biosensors & Bioelectronics, 22(6), 822-8.
Zhou YL, Tian RH, Zhi JF. Amperometric Biosensor Based On Tyrosinase Immobilized On a Boron-doped Diamond Electrode. Biosens Bioelectron. 2007 Jan 15;22(6):822-8. PubMed PMID: 16621510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amperometric biosensor based on tyrosinase immobilized on a boron-doped diamond electrode. AU - Zhou,Yan Li, AU - Tian,Ru Hai, AU - Zhi,Jin Fang, Y1 - 2006/04/18/ PY - 2005/10/30/received PY - 2006/02/16/revised PY - 2006/03/01/accepted PY - 2006/4/20/pubmed PY - 2007/3/7/medline PY - 2006/4/20/entrez SP - 822 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 6 N2 - A novel method has been developed to immobilize tyrosinase onto the surface of boron-doped diamond (BDD) electrode. The hydrogen-terminated BDD (HBDD) surface was first functionalized by photochemically linking vinyl groups of allylamine, producing covalently linked amine-terminated active BDD (ABDD) surface. Then the tyrosinase was immobilized onto the ABDD surface by carbodiimide coupling reaction. The amperometric response was measured as a function of concentration of phenolic compounds in 0.1M phosphate buffer solution (pH 6.5). The tyrosinase-modified ABDD electrode gave a linear response range of 1-175, 1-200 and 1-200 microM and sensitivity of 80.0, 181.4 and 110.0 mA M(-1)cm(-2) for phenol, p-cresol, 4-chlorophenol, respectively. Moreover, selective detection of dopamine (DA) in the presence of ascorbic acid (AA) has been demonstrated with the tyrosinase-modified ABDD electrode. Linearity was observed within the range of 5-120 microM. The above enzyme electrode could maintain 90% of its original activity after intermittent use for 1 month when storing in a dry state at 4 degrees C. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16621510/Amperometric_biosensor_based_on_tyrosinase_immobilized_on_a_boron_doped_diamond_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00091-1 DB - PRIME DP - Unbound Medicine ER -