Tags

Type your tag names separated by a space and hit enter

Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of Brucella abortus in BALB/c mice.
Infect Immun. 2006 May; 74(5):2734-41.II

Abstract

This study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion DNA vaccine encoding both the Brucella abortus L7/L12 protein (ribosomal protein) and Omp16 protein (outer membrane lipoprotein), designated pcDNA3.1-L7/L12-Omp16. Intramuscular injection of this divalent DNA vaccine into BALB/c mice elicited markedly both humoral and cellular immune responses. The specific antibodies exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the dual-gene DNA vaccine elicited a strong T-cell proliferative response and induced a large amount of gamma interferon-producing T cells upon restimulation in vitro with recombinant fusion protein L7/L12-Omp16, suggesting the induction of a typical T-helper-1-dominated immune response in vivo. This divalent DNA vaccine could also induce a significant level of protection against challenge with the virulent strain B. abortus 544 in BALB/c mice. Furthermore, the protection level induced by the divalent DNA vaccine was significantly higher than that induced by the univalent DNA vaccines pcDNA3.1-L7/L12 or pcDNA3.1-Omp16. Taken together, the results of this study verify for the first time that the Omp16 gene can be a candidate target for a DNA vaccine against brucellosis. Additionally, a divalent genetic vaccine based on the L7/L12 and Omp16 genes can elicit a stronger cellular immune response and better immunoprotection than the relevant univalent vaccines can.

Authors+Show Affiliations

State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16622210

Citation

Luo, Deyan, et al. "Protective Immunity Elicited By a Divalent DNA Vaccine Encoding Both the L7/L12 and Omp16 Genes of Brucella Abortus in BALB/c Mice." Infection and Immunity, vol. 74, no. 5, 2006, pp. 2734-41.
Luo D, Ni B, Li P, et al. Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of Brucella abortus in BALB/c mice. Infect Immun. 2006;74(5):2734-41.
Luo, D., Ni, B., Li, P., Shi, W., Zhang, S., Han, Y., Mao, L., He, Y., Wu, Y., & Wang, X. (2006). Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of Brucella abortus in BALB/c mice. Infection and Immunity, 74(5), 2734-41.
Luo D, et al. Protective Immunity Elicited By a Divalent DNA Vaccine Encoding Both the L7/L12 and Omp16 Genes of Brucella Abortus in BALB/c Mice. Infect Immun. 2006;74(5):2734-41. PubMed PMID: 16622210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of Brucella abortus in BALB/c mice. AU - Luo,Deyan, AU - Ni,Bing, AU - Li,Peng, AU - Shi,Wei, AU - Zhang,Songle, AU - Han,Yue, AU - Mao,Liwei, AU - He,Yangdong, AU - Wu,Yuzhang, AU - Wang,Xiliang, PY - 2006/4/20/pubmed PY - 2006/5/24/medline PY - 2006/4/20/entrez SP - 2734 EP - 41 JF - Infection and immunity JO - Infect. Immun. VL - 74 IS - 5 N2 - This study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion DNA vaccine encoding both the Brucella abortus L7/L12 protein (ribosomal protein) and Omp16 protein (outer membrane lipoprotein), designated pcDNA3.1-L7/L12-Omp16. Intramuscular injection of this divalent DNA vaccine into BALB/c mice elicited markedly both humoral and cellular immune responses. The specific antibodies exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the dual-gene DNA vaccine elicited a strong T-cell proliferative response and induced a large amount of gamma interferon-producing T cells upon restimulation in vitro with recombinant fusion protein L7/L12-Omp16, suggesting the induction of a typical T-helper-1-dominated immune response in vivo. This divalent DNA vaccine could also induce a significant level of protection against challenge with the virulent strain B. abortus 544 in BALB/c mice. Furthermore, the protection level induced by the divalent DNA vaccine was significantly higher than that induced by the univalent DNA vaccines pcDNA3.1-L7/L12 or pcDNA3.1-Omp16. Taken together, the results of this study verify for the first time that the Omp16 gene can be a candidate target for a DNA vaccine against brucellosis. Additionally, a divalent genetic vaccine based on the L7/L12 and Omp16 genes can elicit a stronger cellular immune response and better immunoprotection than the relevant univalent vaccines can. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/16622210/Protective_immunity_elicited_by_a_divalent_DNA_vaccine_encoding_both_the_L7/L12_and_Omp16_genes_of_Brucella_abortus_in_BALB/c_mice_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=16622210 DB - PRIME DP - Unbound Medicine ER -