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[Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene].
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Apr; 32(2):209-16.ZW

Abstract

The amylose content of rice caryopsis is determined by Wx protein, one kind of granule-bound starch synthetase which is encoded by Wx gene. Different rice types and species have different levels of Wx gene expression and have different amylose contents in their caryopsis. Wuyunjing No.7 (2200), the japonica rice with an amylose content 17% and its transgenic rice lines with antisense Wx gene (2201 and 2203, with amylose contents 8.5% and 2% respectively), and Longtefu (LP03), the indica rice with a high amylose content (28%) and its transgenic rice with antisense Wx gene (A199, with an amylose content 9%) were used to investigate the effects of Wx protein content decrease on the activities of enzymes involved in starch synthesis and thereby starch accumulation. The results indicated that with the decrease in Wx protein, the amylose content in transgenic caryopsis was reduced accordingly, whereas the amylopectin content per caryopsis (mg/grain) was not affected, and made the total starch content in transgenic caryopsis markedly lower than their parents. With the development of caryopsis, the amylose/total starch ratio was not changed significantly in the two parent caryopses, LP03 and 2200, but it went down gradually in their transgenic caryopses. The amylose/total starch ratio in transgenic caryopses was very significantly lower than their parents in the same period. The activities of ADP-glucose pyrophosphorylase (ADPG-PPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) rose rapidly in early periods of grain filling, and soon reached their maximum, then reduced quickly until the middle and later periods of grain filling. Compared with the parents, the GBSS activity in transgenic caryopsis was significantly lowered, and correlated with the amylose reduction. Besides, the maximum activity of GBSS appeared earlier, and the range of the activity was smaller. In transgenic caryopsis, the activities of ADPG-PPase and SSS were higher than their parents in early and middle periods of grain filling, while the SBE activity was higher than their parents in middle and late periods.

Authors+Show Affiliations

Chen G
Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Agricultural College, Yangzhou University, Yangzhou 225009, China.
Wang Z
No affiliation info available
Liu QQ
No affiliation info available
Xiong F
No affiliation info available
Gu YJ
No affiliation info available
Gu GJ
No affiliation info available

MeSH

1,4-alpha-Glucan Branching EnzymeAmyloseBlotting, NorthernDNA, AntisenseGene Expression Regulation, EnzymologicGene Expression Regulation, PlantGlucose-1-Phosphate AdenylyltransferaseOryzaPlant ProteinsPlants, Genetically ModifiedStarchStarch SynthaseTime Factors

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16622321

Citation

Chen, Gang, et al. "[Changes in the Activities of Enzymes Involved in Starch Synthesis and Accumulation in Caryopsis of Transgenic Rice With Antisense Wx Gene]." Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao = Journal of Plant Physiology and Molecular Biology, vol. 32, no. 2, 2006, pp. 209-16.
Chen G, Wang Z, Liu QQ, et al. [Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene]. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006;32(2):209-16.
Chen, G., Wang, Z., Liu, Q. Q., Xiong, F., Gu, Y. J., & Gu, G. J. (2006). [Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene]. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao = Journal of Plant Physiology and Molecular Biology, 32(2), 209-16.
Chen G, et al. [Changes in the Activities of Enzymes Involved in Starch Synthesis and Accumulation in Caryopsis of Transgenic Rice With Antisense Wx Gene]. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006;32(2):209-16. PubMed PMID: 16622321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene]. AU - Chen,Gang, AU - Wang,Zhong, AU - Liu,Qiao-Quan, AU - Xiong,Fei, AU - Gu,Yun-Jie, AU - Gu,Guo-Jun, PY - 2006/4/20/pubmed PY - 2009/3/20/medline PY - 2006/4/20/entrez SP - 209 EP - 16 JF - Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology JO - Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao VL - 32 IS - 2 N2 - The amylose content of rice caryopsis is determined by Wx protein, one kind of granule-bound starch synthetase which is encoded by Wx gene. Different rice types and species have different levels of Wx gene expression and have different amylose contents in their caryopsis. Wuyunjing No.7 (2200), the japonica rice with an amylose content 17% and its transgenic rice lines with antisense Wx gene (2201 and 2203, with amylose contents 8.5% and 2% respectively), and Longtefu (LP03), the indica rice with a high amylose content (28%) and its transgenic rice with antisense Wx gene (A199, with an amylose content 9%) were used to investigate the effects of Wx protein content decrease on the activities of enzymes involved in starch synthesis and thereby starch accumulation. The results indicated that with the decrease in Wx protein, the amylose content in transgenic caryopsis was reduced accordingly, whereas the amylopectin content per caryopsis (mg/grain) was not affected, and made the total starch content in transgenic caryopsis markedly lower than their parents. With the development of caryopsis, the amylose/total starch ratio was not changed significantly in the two parent caryopses, LP03 and 2200, but it went down gradually in their transgenic caryopses. The amylose/total starch ratio in transgenic caryopses was very significantly lower than their parents in the same period. The activities of ADP-glucose pyrophosphorylase (ADPG-PPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) rose rapidly in early periods of grain filling, and soon reached their maximum, then reduced quickly until the middle and later periods of grain filling. Compared with the parents, the GBSS activity in transgenic caryopsis was significantly lowered, and correlated with the amylose reduction. Besides, the maximum activity of GBSS appeared earlier, and the range of the activity was smaller. In transgenic caryopsis, the activities of ADPG-PPase and SSS were higher than their parents in early and middle periods of grain filling, while the SBE activity was higher than their parents in middle and late periods. SN - 1671-3877 UR - https://www.unboundmedicine.com/medline/citation/16622321/[Changes_in_the_activities_of_enzymes_involved_in_starch_synthesis_and_accumulation_in_caryopsis_of_transgenic_rice_with_antisense_Wx_gene]_ DB - PRIME DP - Unbound Medicine ER -