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Piperine inhibition of 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells.
Eur J Pharmacol. 2006 May 10; 537(1-3):37-44.EJ

Abstract

The effect of alkaloid piperine against the toxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in differentiated PC12 cells was assessed. Piperine treatment revealed a differential effect on the cytotoxicity of MPP(+) and had its maximum inhibitory effect at 1 microM. The addition of piperine (0.5-10 microM) significantly reduced the MPP(+)-induced nuclear damage, mitochondrial membrane permeability changes, formation of reactive oxygen species and depletion of GSH. In contrast, piperine at 50-100 microM showed cytotoxicity and exhibited an additive effect against the MPP(+) toxicity. The results indicate that piperine had a differential effect on the cytotoxicity of MPP(+) depending on concentration. Piperine at low concentrations may reduce the MPP(+)-induced viability loss in PC12 cells by suppressing the changes in the mitochondrial membrane permeability, leading to the release of cytochrome c and subsequent activation of caspase-3. The effects may be ascribed to its inhibitory action on the formation of reactive oxygen species and depletion of GSH.

Authors+Show Affiliations

Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea. leecs@cau.ac.krNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16624279

Citation

Lee, Chung Soo, et al. "Piperine Inhibition of 1-methyl-4-phenylpyridinium-induced Mitochondrial Dysfunction and Cell Death in PC12 Cells." European Journal of Pharmacology, vol. 537, no. 1-3, 2006, pp. 37-44.
Lee CS, Han ES, Kim YK. Piperine inhibition of 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells. Eur J Pharmacol. 2006;537(1-3):37-44.
Lee, C. S., Han, E. S., & Kim, Y. K. (2006). Piperine inhibition of 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells. European Journal of Pharmacology, 537(1-3), 37-44.
Lee CS, Han ES, Kim YK. Piperine Inhibition of 1-methyl-4-phenylpyridinium-induced Mitochondrial Dysfunction and Cell Death in PC12 Cells. Eur J Pharmacol. 2006 May 10;537(1-3):37-44. PubMed PMID: 16624279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Piperine inhibition of 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells. AU - Lee,Chung Soo, AU - Han,Eun Sook, AU - Kim,Young Ki, Y1 - 2006/03/20/ PY - 2005/11/21/received PY - 2006/03/02/revised PY - 2006/03/13/accepted PY - 2006/4/21/pubmed PY - 2006/8/12/medline PY - 2006/4/21/entrez SP - 37 EP - 44 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 537 IS - 1-3 N2 - The effect of alkaloid piperine against the toxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in differentiated PC12 cells was assessed. Piperine treatment revealed a differential effect on the cytotoxicity of MPP(+) and had its maximum inhibitory effect at 1 microM. The addition of piperine (0.5-10 microM) significantly reduced the MPP(+)-induced nuclear damage, mitochondrial membrane permeability changes, formation of reactive oxygen species and depletion of GSH. In contrast, piperine at 50-100 microM showed cytotoxicity and exhibited an additive effect against the MPP(+) toxicity. The results indicate that piperine had a differential effect on the cytotoxicity of MPP(+) depending on concentration. Piperine at low concentrations may reduce the MPP(+)-induced viability loss in PC12 cells by suppressing the changes in the mitochondrial membrane permeability, leading to the release of cytochrome c and subsequent activation of caspase-3. The effects may be ascribed to its inhibitory action on the formation of reactive oxygen species and depletion of GSH. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/16624279/Piperine_inhibition_of_1_methyl_4_phenylpyridinium_induced_mitochondrial_dysfunction_and_cell_death_in_PC12_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(06)00282-2 DB - PRIME DP - Unbound Medicine ER -