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Role of 11-beta-hydroxysteroid dehydrogenase type 1 in differentiation of 3T3-L1 cells and in rats with diet-induced obesity.
Acta Pharmacol Sin. 2006 May; 27(5):588-96.AP

Abstract

AIM

To observe the roles of 11-beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in in vitro preadipocyte differentiation and in rats with diet-induced obesity (DIO).

METHODS

Protein expression of 11beta-HSD1 in the process of 3T3-L1 cell differentiation and in various tissues of the rats were detected by Western blot analysis; expression of 11beta-HSD1 mRNA and glucocorticoid receptor (GR) and other marker genes of preadipocyte differentiation were detected by using real-time PCR.

RESULTS

Lipid droplets in 3T3-L1 cells accumulated and increased after stimulation. A dramatically elevated protein level of 11beta-HSD1, especially in the late stages of 3T3-L1 cell differentiation, was detected. The relative mRNA levels of 11beta-HSD1, GR and cell differentiation markers LPL, aP2, and FAS were upregulated, and Pref-1 was downregulated during the differentiation. In DIO rats, bodyweight, visceral adipose mass index and the protein expression of 11beta-HSD1 increased, especially in adipose tissue, brain and muscles. Serum insulin, triglyceride, total cholesterol and low-density lipoprotein cholesterol were found to be increased in DIO rats, but without any obvious changes in blood glucose or tumor necrosis factor-alpha levels.

CONCLUSION

11beta-HSD1 may promote preadipocyte differentiation, and may be involved in the development of obesity.

Authors+Show Affiliations

Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16626514

Citation

Liu, Yun, et al. "Role of 11-beta-hydroxysteroid Dehydrogenase Type 1 in Differentiation of 3T3-L1 Cells and in Rats With Diet-induced Obesity." Acta Pharmacologica Sinica, vol. 27, no. 5, 2006, pp. 588-96.
Liu Y, Sun WL, Sun Y, et al. Role of 11-beta-hydroxysteroid dehydrogenase type 1 in differentiation of 3T3-L1 cells and in rats with diet-induced obesity. Acta Pharmacol Sin. 2006;27(5):588-96.
Liu, Y., Sun, W. L., Sun, Y., Hu, G., & Ding, G. X. (2006). Role of 11-beta-hydroxysteroid dehydrogenase type 1 in differentiation of 3T3-L1 cells and in rats with diet-induced obesity. Acta Pharmacologica Sinica, 27(5), 588-96.
Liu Y, et al. Role of 11-beta-hydroxysteroid Dehydrogenase Type 1 in Differentiation of 3T3-L1 Cells and in Rats With Diet-induced Obesity. Acta Pharmacol Sin. 2006;27(5):588-96. PubMed PMID: 16626514.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of 11-beta-hydroxysteroid dehydrogenase type 1 in differentiation of 3T3-L1 cells and in rats with diet-induced obesity. AU - Liu,Yun, AU - Sun,Wen-Lan, AU - Sun,Yan, AU - Hu,Gang, AU - Ding,Guo-Xian, PY - 2006/4/22/pubmed PY - 2006/12/9/medline PY - 2006/4/22/entrez SP - 588 EP - 96 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 27 IS - 5 N2 - AIM: To observe the roles of 11-beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in in vitro preadipocyte differentiation and in rats with diet-induced obesity (DIO). METHODS: Protein expression of 11beta-HSD1 in the process of 3T3-L1 cell differentiation and in various tissues of the rats were detected by Western blot analysis; expression of 11beta-HSD1 mRNA and glucocorticoid receptor (GR) and other marker genes of preadipocyte differentiation were detected by using real-time PCR. RESULTS: Lipid droplets in 3T3-L1 cells accumulated and increased after stimulation. A dramatically elevated protein level of 11beta-HSD1, especially in the late stages of 3T3-L1 cell differentiation, was detected. The relative mRNA levels of 11beta-HSD1, GR and cell differentiation markers LPL, aP2, and FAS were upregulated, and Pref-1 was downregulated during the differentiation. In DIO rats, bodyweight, visceral adipose mass index and the protein expression of 11beta-HSD1 increased, especially in adipose tissue, brain and muscles. Serum insulin, triglyceride, total cholesterol and low-density lipoprotein cholesterol were found to be increased in DIO rats, but without any obvious changes in blood glucose or tumor necrosis factor-alpha levels. CONCLUSION: 11beta-HSD1 may promote preadipocyte differentiation, and may be involved in the development of obesity. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/16626514/Role_of_11_beta_hydroxysteroid_dehydrogenase_type_1_in_differentiation_of_3T3_L1_cells_and_in_rats_with_diet_induced_obesity_ L2 - https://doi.org/10.1111/j.1745-7254.2006.00316.x DB - PRIME DP - Unbound Medicine ER -