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The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: role of vagal innervation.
Surgery 2006; 139(4):542-9S

Abstract

BACKGROUND

Protein and messenger RNA (mRNA) levels of the hexose transporters sodium-dependent glucose transporter-1, glucose transporter 2, and glucose transporter 5 follow a (daily) diurnal rhythm in rat jejunum. Because vagal innervation mediates the diurnal activity of other proteins in the rat small bowel, we hypothesized that the diurnal variation of mRNA and protein levels of these hexose transport proteins are mediated by vagal innervation.

METHODS

Forty-eight rats kept in a strictly maintained, alternating 12-hour light-dark room underwent either sham laparotomy (n = 24) or bilateral total abdominal vagotomy (n = 24). Four weeks postoperatively, jejunal mucosa was harvested from 6 rats in each group at 3 am, 9 am, 3 pm and 9 pm; mRNA levels were determined by reverse transcription real-time polymerase chain reaction and protein levels by semiquantitative Western blot analysis. Transporter mRNA and protein levels were expressed as a ratio to the corresponding mRNA and protein levels of the stably expressed housekeeping gene glyceraldehyde-6-phosphate dehydrogenase.

RESULTS

mRNA and protein levels for all 3 hexose transporters showed diurnal variation in sham controls (P < or = .01 for all). After vagotomy, although mRNA levels of all 3 transporters showed diurnal variation (each P < .01), diurnal variation in all 3 hexose transporter protein levels was abolished (P > .10 for all).

CONCLUSIONS

Vagal innervation appears to differentially mediate the diurnal changes in hexose transporter mRNA and protein expression in the rat jejunum by posttranscriptional, and/or posttranslational processes.

Authors+Show Affiliations

Department of Surgery, GI Research Unit, Alfred 2, St. Mary's Hospital, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16627065

Citation

Houghton, Scott G., et al. "The Diurnal Periodicity of Hexose Transporter mRNA and Protein Levels in the Rat Jejunum: Role of Vagal Innervation." Surgery, vol. 139, no. 4, 2006, pp. 542-9.
Houghton SG, Zarroug AE, Duenes JA, et al. The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: role of vagal innervation. Surgery. 2006;139(4):542-9.
Houghton, S. G., Zarroug, A. E., Duenes, J. A., Fernandez-Zapico, M. E., & Sarr, M. G. (2006). The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: role of vagal innervation. Surgery, 139(4), pp. 542-9.
Houghton SG, et al. The Diurnal Periodicity of Hexose Transporter mRNA and Protein Levels in the Rat Jejunum: Role of Vagal Innervation. Surgery. 2006;139(4):542-9. PubMed PMID: 16627065.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: role of vagal innervation. AU - Houghton,Scott G, AU - Zarroug,Abdalla E, AU - Duenes,Judith A, AU - Fernandez-Zapico,Martin E, AU - Sarr,Michael G, PY - 2005/06/22/received PY - 2005/09/01/revised PY - 2005/09/10/accepted PY - 2006/4/22/pubmed PY - 2006/7/20/medline PY - 2006/4/22/entrez SP - 542 EP - 9 JF - Surgery JO - Surgery VL - 139 IS - 4 N2 - BACKGROUND: Protein and messenger RNA (mRNA) levels of the hexose transporters sodium-dependent glucose transporter-1, glucose transporter 2, and glucose transporter 5 follow a (daily) diurnal rhythm in rat jejunum. Because vagal innervation mediates the diurnal activity of other proteins in the rat small bowel, we hypothesized that the diurnal variation of mRNA and protein levels of these hexose transport proteins are mediated by vagal innervation. METHODS: Forty-eight rats kept in a strictly maintained, alternating 12-hour light-dark room underwent either sham laparotomy (n = 24) or bilateral total abdominal vagotomy (n = 24). Four weeks postoperatively, jejunal mucosa was harvested from 6 rats in each group at 3 am, 9 am, 3 pm and 9 pm; mRNA levels were determined by reverse transcription real-time polymerase chain reaction and protein levels by semiquantitative Western blot analysis. Transporter mRNA and protein levels were expressed as a ratio to the corresponding mRNA and protein levels of the stably expressed housekeeping gene glyceraldehyde-6-phosphate dehydrogenase. RESULTS: mRNA and protein levels for all 3 hexose transporters showed diurnal variation in sham controls (P < or = .01 for all). After vagotomy, although mRNA levels of all 3 transporters showed diurnal variation (each P < .01), diurnal variation in all 3 hexose transporter protein levels was abolished (P > .10 for all). CONCLUSIONS: Vagal innervation appears to differentially mediate the diurnal changes in hexose transporter mRNA and protein expression in the rat jejunum by posttranscriptional, and/or posttranslational processes. SN - 0039-6060 UR - https://www.unboundmedicine.com/medline/citation/16627065/The_diurnal_periodicity_of_hexose_transporter_mRNA_and_protein_levels_in_the_rat_jejunum:_role_of_vagal_innervation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-6060(05)00501-5 DB - PRIME DP - Unbound Medicine ER -