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Chromosomal integration of plasmid DNA by homologous recombination in Enterococcus faecalis and Lactococcus lactis subsp. lactis hosts harboring Tn919.
Appl Environ Microbiol 1991; 57(9):2677-82AE

Abstract

Integration of pCI192, a pBR322-derived vector plasmid containing homology to the chromosomally located conjugative transposon Tn919 was observed in two strains that harbor Tn919, namely, Enterococcus faecalis GF590 and Lactococcus lactis subsp. lactis CH919. Hybridization analysis indicated that single-copy integration of the plasmid had occurred at low frequency. The Tn919::plasmid structure was conjugated from an E. faecalis donor to a L. lactis recipient, although at lower frequencies than was Tn919. Segregation of the tetracycline and chloramphenicol resistance markers during conjugation was observed. The integration strategy described allows for DNA manipulations to be performed in an easily manipulated model host strain with the subsequent transfer of integrated structures by conjugation to any strain capable of receiving Tn919. The results indicate that homologous recombination events may be used to introduce plasmid-encoded genes to the lactococcal chromosome.

Authors+Show Affiliations

Food Microbiology Department, University College, Cork, Ireland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1662938

Citation

Casey, J, et al. "Chromosomal Integration of Plasmid DNA By Homologous Recombination in Enterococcus Faecalis and Lactococcus Lactis Subsp. Lactis Hosts Harboring Tn919." Applied and Environmental Microbiology, vol. 57, no. 9, 1991, pp. 2677-82.
Casey J, Daly C, Fitzgerald GF. Chromosomal integration of plasmid DNA by homologous recombination in Enterococcus faecalis and Lactococcus lactis subsp. lactis hosts harboring Tn919. Appl Environ Microbiol. 1991;57(9):2677-82.
Casey, J., Daly, C., & Fitzgerald, G. F. (1991). Chromosomal integration of plasmid DNA by homologous recombination in Enterococcus faecalis and Lactococcus lactis subsp. lactis hosts harboring Tn919. Applied and Environmental Microbiology, 57(9), pp. 2677-82.
Casey J, Daly C, Fitzgerald GF. Chromosomal Integration of Plasmid DNA By Homologous Recombination in Enterococcus Faecalis and Lactococcus Lactis Subsp. Lactis Hosts Harboring Tn919. Appl Environ Microbiol. 1991;57(9):2677-82. PubMed PMID: 1662938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosomal integration of plasmid DNA by homologous recombination in Enterococcus faecalis and Lactococcus lactis subsp. lactis hosts harboring Tn919. AU - Casey,J, AU - Daly,C, AU - Fitzgerald,G F, PY - 1991/9/1/pubmed PY - 1991/9/1/medline PY - 1991/9/1/entrez SP - 2677 EP - 82 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 57 IS - 9 N2 - Integration of pCI192, a pBR322-derived vector plasmid containing homology to the chromosomally located conjugative transposon Tn919 was observed in two strains that harbor Tn919, namely, Enterococcus faecalis GF590 and Lactococcus lactis subsp. lactis CH919. Hybridization analysis indicated that single-copy integration of the plasmid had occurred at low frequency. The Tn919::plasmid structure was conjugated from an E. faecalis donor to a L. lactis recipient, although at lower frequencies than was Tn919. Segregation of the tetracycline and chloramphenicol resistance markers during conjugation was observed. The integration strategy described allows for DNA manipulations to be performed in an easily manipulated model host strain with the subsequent transfer of integrated structures by conjugation to any strain capable of receiving Tn919. The results indicate that homologous recombination events may be used to introduce plasmid-encoded genes to the lactococcal chromosome. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/1662938/Chromosomal_integration_of_plasmid_DNA_by_homologous_recombination_in_Enterococcus_faecalis_and_Lactococcus_lactis_subsp__lactis_hosts_harboring_Tn919_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=1662938 DB - PRIME DP - Unbound Medicine ER -