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RNase Z in Escherichia coli plays a significant role in mRNA decay.
Mol Microbiol. 2006 May; 60(3):723-37.MM

Abstract

Genetic and biochemical analysis of RNase Z in eukaryotes, such as Arabadopsis thaliana, and prokaryotes like Bacillus subtilis have demonstrated that this endoribonuclease is essential for the maturation of tRNA precursors that do not contain a chromosomally encoded CCA determinant. As all Escherichia coli tRNA transcripts have chromosomally encoded CCA determinants, the function of its putative RNase Z homologue, the product of the elaC gene, is not clear. Here we demonstrate that the E. coli ElaC protein (RNase Z) endonucleolytically processes B. subtilis tRNA precursors lacking a CCA determinant both in vivo and in vitro. More importantly, E. coli RNase Z plays a significant role in mRNA decay, a previously unidentified activity for the enzyme. The purified RNase Z protein cleaves the rpsT mRNA at locations distinct from those obtained with RNase E. As expected, under physiological conditions E. coli and B. subtilis tRNA precursors containing a CCA determinant are not substrates. These results suggest a potentially important new role for the RNase Z family of proteins in RNA metabolism, particularly in organisms lacking RNase E.

Authors+Show Affiliations

Department of Genetics, University of Georgia, Athens, GA 30602, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16629673

Citation

Perwez, Tariq, and Sidney R. Kushner. "RNase Z in Escherichia Coli Plays a Significant Role in mRNA Decay." Molecular Microbiology, vol. 60, no. 3, 2006, pp. 723-37.
Perwez T, Kushner SR. RNase Z in Escherichia coli plays a significant role in mRNA decay. Mol Microbiol. 2006;60(3):723-37.
Perwez, T., & Kushner, S. R. (2006). RNase Z in Escherichia coli plays a significant role in mRNA decay. Molecular Microbiology, 60(3), 723-37.
Perwez T, Kushner SR. RNase Z in Escherichia Coli Plays a Significant Role in mRNA Decay. Mol Microbiol. 2006;60(3):723-37. PubMed PMID: 16629673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNase Z in Escherichia coli plays a significant role in mRNA decay. AU - Perwez,Tariq, AU - Kushner,Sidney R, PY - 2006/4/25/pubmed PY - 2006/7/4/medline PY - 2006/4/25/entrez SP - 723 EP - 37 JF - Molecular microbiology JO - Mol Microbiol VL - 60 IS - 3 N2 - Genetic and biochemical analysis of RNase Z in eukaryotes, such as Arabadopsis thaliana, and prokaryotes like Bacillus subtilis have demonstrated that this endoribonuclease is essential for the maturation of tRNA precursors that do not contain a chromosomally encoded CCA determinant. As all Escherichia coli tRNA transcripts have chromosomally encoded CCA determinants, the function of its putative RNase Z homologue, the product of the elaC gene, is not clear. Here we demonstrate that the E. coli ElaC protein (RNase Z) endonucleolytically processes B. subtilis tRNA precursors lacking a CCA determinant both in vivo and in vitro. More importantly, E. coli RNase Z plays a significant role in mRNA decay, a previously unidentified activity for the enzyme. The purified RNase Z protein cleaves the rpsT mRNA at locations distinct from those obtained with RNase E. As expected, under physiological conditions E. coli and B. subtilis tRNA precursors containing a CCA determinant are not substrates. These results suggest a potentially important new role for the RNase Z family of proteins in RNA metabolism, particularly in organisms lacking RNase E. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/16629673/RNase_Z_in_Escherichia_coli_plays_a_significant_role_in_mRNA_decay_ L2 - https://doi.org/10.1111/j.1365-2958.2006.05124.x DB - PRIME DP - Unbound Medicine ER -