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Barley and rye prolamins induce an mRNA interferon-gamma response in coeliac mucosa.
Aliment Pharmacol Ther. 2006 May 01; 23(9):1307-14.AP

Abstract

BACKGROUND

In coeliac disease, wheat, barley and rye are traditionally excluded in the gluten-free diet. However, few studies have examined the small intestinal immune response to barley and rye.

AIM

To investigate the immunogenicity of barley and rye prolamins (hordein and secalin respectively) in comparison with wheat gliadin.

METHODS

Duodenal biopsies from 22 coeliac patients and 23 disease controls were cultured for 4 h with gliadin, hordein or secalin and compared with culture medium alone. Proinflammatory cytokines, interferon-gamma and interleukin-2, were quantified by TaqMan polymerase chain reaction and enzyme-linked immunosorbent assay.

RESULTS

Hordein caused the greatest increase in interferon-gamma mRNA in coeliac patients (median: 3.3-fold) in comparison with control subjects (median: 0.28-fold, P < 0.085). Secalin and gliadin induced similar levels of interferon-gamma mRNA with median fold-changes of 3.4 and 2.8, respectively, in coeliac patients in comparison with 1.6- and 1.1-fold increases in control subjects (P < 0.294 and P < 0.105, respectively). The median fold-changes for interleukin-2 mRNA did not differ between coeliac patients and controls. Cytokine protein was not upregulated.

CONCLUSION

The findings of this study provide evidence that barley and rye cause immune activation in the mucosa of coeliac patients and support the practice that barley and rye should be excluded from the gluten-free diet.

Authors+Show Affiliations

Department of Immunology, Institute of Molecular Medicine, Trinity College Centre for Health Sciences, St James's Hospital, Dublin, Ireland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16629935

Citation

Bracken, S C., et al. "Barley and Rye Prolamins Induce an mRNA Interferon-gamma Response in Coeliac Mucosa." Alimentary Pharmacology & Therapeutics, vol. 23, no. 9, 2006, pp. 1307-14.
Bracken SC, Kilmartin C, Wieser H, et al. Barley and rye prolamins induce an mRNA interferon-gamma response in coeliac mucosa. Aliment Pharmacol Ther. 2006;23(9):1307-14.
Bracken, S. C., Kilmartin, C., Wieser, H., Jackson, J., & Feighery, C. (2006). Barley and rye prolamins induce an mRNA interferon-gamma response in coeliac mucosa. Alimentary Pharmacology & Therapeutics, 23(9), 1307-14.
Bracken SC, et al. Barley and Rye Prolamins Induce an mRNA Interferon-gamma Response in Coeliac Mucosa. Aliment Pharmacol Ther. 2006 May 1;23(9):1307-14. PubMed PMID: 16629935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Barley and rye prolamins induce an mRNA interferon-gamma response in coeliac mucosa. AU - Bracken,S C, AU - Kilmartin,C, AU - Wieser,H, AU - Jackson,J, AU - Feighery,C, PY - 2006/4/25/pubmed PY - 2006/9/16/medline PY - 2006/4/25/entrez SP - 1307 EP - 14 JF - Alimentary pharmacology & therapeutics JO - Aliment Pharmacol Ther VL - 23 IS - 9 N2 - BACKGROUND: In coeliac disease, wheat, barley and rye are traditionally excluded in the gluten-free diet. However, few studies have examined the small intestinal immune response to barley and rye. AIM: To investigate the immunogenicity of barley and rye prolamins (hordein and secalin respectively) in comparison with wheat gliadin. METHODS: Duodenal biopsies from 22 coeliac patients and 23 disease controls were cultured for 4 h with gliadin, hordein or secalin and compared with culture medium alone. Proinflammatory cytokines, interferon-gamma and interleukin-2, were quantified by TaqMan polymerase chain reaction and enzyme-linked immunosorbent assay. RESULTS: Hordein caused the greatest increase in interferon-gamma mRNA in coeliac patients (median: 3.3-fold) in comparison with control subjects (median: 0.28-fold, P < 0.085). Secalin and gliadin induced similar levels of interferon-gamma mRNA with median fold-changes of 3.4 and 2.8, respectively, in coeliac patients in comparison with 1.6- and 1.1-fold increases in control subjects (P < 0.294 and P < 0.105, respectively). The median fold-changes for interleukin-2 mRNA did not differ between coeliac patients and controls. Cytokine protein was not upregulated. CONCLUSION: The findings of this study provide evidence that barley and rye cause immune activation in the mucosa of coeliac patients and support the practice that barley and rye should be excluded from the gluten-free diet. SN - 0269-2813 UR - https://www.unboundmedicine.com/medline/citation/16629935/Barley_and_rye_prolamins_induce_an_mRNA_interferon_gamma_response_in_coeliac_mucosa_ L2 - https://doi.org/10.1111/j.1365-2036.2006.02876.x DB - PRIME DP - Unbound Medicine ER -