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Heterologous protein production in the yeast Kluyveromyces lactis.
FEMS Yeast Res. 2006 May; 6(3):381-92.FY

Abstract

Kluyveromyces lactis is both scientifically and biotechnologically one of the most important non-Saccharomyces yeasts. Its biotechnological significance builds on its history of safe use in the food industry and its well-known ability to produce enzymes like lactase and bovine chymosin on an industrial scale. In this article, we review the various strains, genetic techniques and molecular tools currently available for the use of K. lactis as a host for protein expression. Additionally, we present data illustrating the recent use of proteomics studies to identify cellular bottlenecks that impede heterologous protein expression.

Authors+Show Affiliations

van Ooyen AJ
DSM Food Specialties, Delft, The Netherlands. Ooyen-van@dsm.com
Dekker P
No affiliation info available
Huang M
No affiliation info available
Olsthoorn MM
No affiliation info available
Jacobs DI
No affiliation info available
Colussi PA
No affiliation info available
Taron CH
No affiliation info available

MeSH

Fungal ProteinsGenetic EngineeringIndustrial MicrobiologyKluyveromycesRecombinant Proteins

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

16630278

Citation

van Ooyen, Albert J J., et al. "Heterologous Protein Production in the Yeast Kluyveromyces Lactis." FEMS Yeast Research, vol. 6, no. 3, 2006, pp. 381-92.
van Ooyen AJ, Dekker P, Huang M, et al. Heterologous protein production in the yeast Kluyveromyces lactis. FEMS Yeast Res. 2006;6(3):381-92.
van Ooyen, A. J., Dekker, P., Huang, M., Olsthoorn, M. M., Jacobs, D. I., Colussi, P. A., & Taron, C. H. (2006). Heterologous protein production in the yeast Kluyveromyces lactis. FEMS Yeast Research, 6(3), 381-92.
van Ooyen AJ, et al. Heterologous Protein Production in the Yeast Kluyveromyces Lactis. FEMS Yeast Res. 2006;6(3):381-92. PubMed PMID: 16630278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterologous protein production in the yeast Kluyveromyces lactis. AU - van Ooyen,Albert J J, AU - Dekker,Peter, AU - Huang,Michael, AU - Olsthoorn,Maurien M A, AU - Jacobs,Denise I, AU - Colussi,Paul A, AU - Taron,Christopher H, PY - 2006/4/25/pubmed PY - 2006/9/22/medline PY - 2006/4/25/entrez SP - 381 EP - 92 JF - FEMS yeast research JO - FEMS Yeast Res VL - 6 IS - 3 N2 - Kluyveromyces lactis is both scientifically and biotechnologically one of the most important non-Saccharomyces yeasts. Its biotechnological significance builds on its history of safe use in the food industry and its well-known ability to produce enzymes like lactase and bovine chymosin on an industrial scale. In this article, we review the various strains, genetic techniques and molecular tools currently available for the use of K. lactis as a host for protein expression. Additionally, we present data illustrating the recent use of proteomics studies to identify cellular bottlenecks that impede heterologous protein expression. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/16630278/Heterologous_protein_production_in_the_yeast_Kluyveromyces_lactis_ DB - PRIME DP - Unbound Medicine ER -