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Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells.
Biochem Biophys Res Commun. 2006 Jun 09; 344(3):912-9.BB

Abstract

We examined whether PDGF may directly stimulate the expression of VEGF by retinal pigment epithelial (RPE) cells in vitro, and the involvement of three signal transduction pathways in the regulation of PDGF-evoked cell proliferation, migration, and production of VEGF-A was investigated. PDGF stimulated the gene and protein expression of VEGF-A by RPE cells, and increased cell proliferation and chemotaxis. PDGF activated all signaling pathways investigated, as determined by increased phosphorylation levels of ERK1/2, p38, and Akt proteins. The three signaling pathways were involved in the mediation of PDGF-evoked cell proliferation, while p38 and PI3K mediated cell migration, and PI3K mediated secretion of VEGF-A. In addition to VEGF-A, the cells expressed mRNAs for various members of the VEGF family and for their receptors, including VEGF-B, -C, -D, flt-1, and KDR. The data indicate that PDGF selectively stimulates the expression of VEGF-A in RPE cells. PDGF evokes at least three signal transduction pathways which are differentially involved in various cellular responses.

Authors+Show Affiliations

Department of Ophthalmology and Eye Clinic, University of Leipzig Medical Faculty, 04103 Leipzig, Germany. hollbm@medizin.uni-leipzig.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16631611

Citation

Hollborn, Margrit, et al. "Signaling Pathways Involved in PDGF-evoked Cellular Responses in Human RPE Cells." Biochemical and Biophysical Research Communications, vol. 344, no. 3, 2006, pp. 912-9.
Hollborn M, Bringmann A, Faude F, et al. Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells. Biochem Biophys Res Commun. 2006;344(3):912-9.
Hollborn, M., Bringmann, A., Faude, F., Wiedemann, P., & Kohen, L. (2006). Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells. Biochemical and Biophysical Research Communications, 344(3), 912-9.
Hollborn M, et al. Signaling Pathways Involved in PDGF-evoked Cellular Responses in Human RPE Cells. Biochem Biophys Res Commun. 2006 Jun 9;344(3):912-9. PubMed PMID: 16631611.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Signaling pathways involved in PDGF-evoked cellular responses in human RPE cells. AU - Hollborn,Margrit, AU - Bringmann,Andreas, AU - Faude,Frank, AU - Wiedemann,Peter, AU - Kohen,Leon, Y1 - 2006/04/06/ PY - 2006/03/23/received PY - 2006/03/25/accepted PY - 2006/4/25/pubmed PY - 2006/6/23/medline PY - 2006/4/25/entrez SP - 912 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 344 IS - 3 N2 - We examined whether PDGF may directly stimulate the expression of VEGF by retinal pigment epithelial (RPE) cells in vitro, and the involvement of three signal transduction pathways in the regulation of PDGF-evoked cell proliferation, migration, and production of VEGF-A was investigated. PDGF stimulated the gene and protein expression of VEGF-A by RPE cells, and increased cell proliferation and chemotaxis. PDGF activated all signaling pathways investigated, as determined by increased phosphorylation levels of ERK1/2, p38, and Akt proteins. The three signaling pathways were involved in the mediation of PDGF-evoked cell proliferation, while p38 and PI3K mediated cell migration, and PI3K mediated secretion of VEGF-A. In addition to VEGF-A, the cells expressed mRNAs for various members of the VEGF family and for their receptors, including VEGF-B, -C, -D, flt-1, and KDR. The data indicate that PDGF selectively stimulates the expression of VEGF-A in RPE cells. PDGF evokes at least three signal transduction pathways which are differentially involved in various cellular responses. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/16631611/Signaling_pathways_involved_in_PDGF_evoked_cellular_responses_in_human_RPE_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(06)00748-0 DB - PRIME DP - Unbound Medicine ER -