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Separation and characterization of silybin, isosilybin, silydianin and silychristin in milk thistle extract by liquid chromatography-electrospray tandem mass spectrometry.
J Chromatogr A 2006; 1116(1-2):57-68JC

Abstract

A selective and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed for the characterization of silymarin in commercially available milk thistle extract. In this study, six main active constituents, including silydianin, silychristin, diastereomers of silybin (silybin A and B) and diastereomers of isosilybin (isosilybin A and B) in silymarin, were completely separated on a YMC ODS-AQ HPLC column using a gradient mobile phase system comprised of ammonium acetate and methanol/water/formic acid. Identification and characterization of the major constituents were based not only on the product ion scan, which provided unique fragmentation information of a selected molecular ion, but also on the specific fragmentation of multiple reaction monitoring (MRM) data, which confirmed the retention times of LC chromatographic peaks. The method was applied in the analysis of human plasma samples in the presence of silymarin and appeared to be suitable for the pharmacokinetic studies in which the discrimination of silymarin constituents is essential.

Authors+Show Affiliations

Division of Clinical Pharmacology & Therapeutics, The Children's Hospital of Philadelphia, Abramson Research Center 916H, Philadelphia, PA 19104-4399, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16631762

Citation

Lee, James I., et al. "Separation and Characterization of Silybin, Isosilybin, Silydianin and Silychristin in Milk Thistle Extract By Liquid Chromatography-electrospray Tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1116, no. 1-2, 2006, pp. 57-68.
Lee JI, Hsu BH, Wu D, et al. Separation and characterization of silybin, isosilybin, silydianin and silychristin in milk thistle extract by liquid chromatography-electrospray tandem mass spectrometry. J Chromatogr A. 2006;1116(1-2):57-68.
Lee, J. I., Hsu, B. H., Wu, D., & Barrett, J. S. (2006). Separation and characterization of silybin, isosilybin, silydianin and silychristin in milk thistle extract by liquid chromatography-electrospray tandem mass spectrometry. Journal of Chromatography. A, 1116(1-2), pp. 57-68.
Lee JI, et al. Separation and Characterization of Silybin, Isosilybin, Silydianin and Silychristin in Milk Thistle Extract By Liquid Chromatography-electrospray Tandem Mass Spectrometry. J Chromatogr A. 2006 May 26;1116(1-2):57-68. PubMed PMID: 16631762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and characterization of silybin, isosilybin, silydianin and silychristin in milk thistle extract by liquid chromatography-electrospray tandem mass spectrometry. AU - Lee,James I, AU - Hsu,Bih H, AU - Wu,Di, AU - Barrett,Jeffrey S, Y1 - 2006/04/24/ PY - 2006/01/03/received PY - 2006/03/02/revised PY - 2006/03/07/accepted PY - 2006/4/25/pubmed PY - 2006/7/6/medline PY - 2006/4/25/entrez SP - 57 EP - 68 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1116 IS - 1-2 N2 - A selective and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed for the characterization of silymarin in commercially available milk thistle extract. In this study, six main active constituents, including silydianin, silychristin, diastereomers of silybin (silybin A and B) and diastereomers of isosilybin (isosilybin A and B) in silymarin, were completely separated on a YMC ODS-AQ HPLC column using a gradient mobile phase system comprised of ammonium acetate and methanol/water/formic acid. Identification and characterization of the major constituents were based not only on the product ion scan, which provided unique fragmentation information of a selected molecular ion, but also on the specific fragmentation of multiple reaction monitoring (MRM) data, which confirmed the retention times of LC chromatographic peaks. The method was applied in the analysis of human plasma samples in the presence of silymarin and appeared to be suitable for the pharmacokinetic studies in which the discrimination of silymarin constituents is essential. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16631762/Separation_and_characterization_of_silybin_isosilybin_silydianin_and_silychristin_in_milk_thistle_extract_by_liquid_chromatography_electrospray_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)00574-7 DB - PRIME DP - Unbound Medicine ER -