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Exhaled markers of oxidative stress in idiopathic pulmonary fibrosis.
Eur J Clin Invest. 2006 May; 36(5):362-7.EJ

Abstract

BACKGROUND

Expired breath condensate (EBC) has never been used to explore the level of oxidative stress in idiopathic pulmonary fibrosis (IPF). Therefore, the aim of this study was to measure the levels of H2O2 and 8-isoprostane, as biomarkers of oxidative stress, in the EBC of patients with IPF.

MATERIALS AND METHODS

We investigated 16 patients with IPF and 15 healthy subjects as the control group. The levels of H2O2 and 8-isoprostane were measured in the EBC of all subjects and were compared between the IPF and control groups. In patients with IPF, H2O2 and 8-isoprostane were further correlated with pulmonary function tests (PFTs), the resting pO2 and the differential cell count from the bronchoalveolar lavage fluid (BALF).

RESULTS

The mean (95%CI) concentration of H2O2 was increased in the patients with IPF compared with the normal subjects (0.36, 0.24-0.47 microM vs. 0.16, 0.10-0.23 microM, P=0.003). The mean (95%CI) concentration of 8-isoprostane was also increased in the patients with IPF compared with the controls (74, 38-110 pg mL-1 vs. 33, 28-39 pg mL-1, P=0.02). In the patients with IPF, the diffusing capacity of the lung for carbon monoxide was negatively correlated with the levels of H2O2 in EBC (P=0.03, r=-0.58). No other correlation was found between the oxidative stress markers in the EBC and PFT values, pO2 or BALF cell count.

CONCLUSIONS

Our data suggest that H2O2 and 8-isoprostane are increased in the EBC of patients with IPF. H2O2 may be correlated with the severity of the disease in IPF.

Authors+Show Affiliations

Department of Pneumonology and the Clinical Research Unit, Army General Hospital of Athens, and Department of Pneumonology, University Hospital of Heraklion, Greece. kpsazakis@hol.grNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16634841

Citation

Psathakis, K, et al. "Exhaled Markers of Oxidative Stress in Idiopathic Pulmonary Fibrosis." European Journal of Clinical Investigation, vol. 36, no. 5, 2006, pp. 362-7.
Psathakis K, Mermigkis D, Papatheodorou G, et al. Exhaled markers of oxidative stress in idiopathic pulmonary fibrosis. Eur J Clin Invest. 2006;36(5):362-7.
Psathakis, K., Mermigkis, D., Papatheodorou, G., Loukides, S., Panagou, P., Polychronopoulos, V., Siafakas, N. M., & Bouros, D. (2006). Exhaled markers of oxidative stress in idiopathic pulmonary fibrosis. European Journal of Clinical Investigation, 36(5), 362-7.
Psathakis K, et al. Exhaled Markers of Oxidative Stress in Idiopathic Pulmonary Fibrosis. Eur J Clin Invest. 2006;36(5):362-7. PubMed PMID: 16634841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exhaled markers of oxidative stress in idiopathic pulmonary fibrosis. AU - Psathakis,K, AU - Mermigkis,D, AU - Papatheodorou,G, AU - Loukides,S, AU - Panagou,P, AU - Polychronopoulos,V, AU - Siafakas,N M, AU - Bouros,D, PY - 2006/4/26/pubmed PY - 2006/9/16/medline PY - 2006/4/26/entrez SP - 362 EP - 7 JF - European journal of clinical investigation JO - Eur. J. Clin. Invest. VL - 36 IS - 5 N2 - BACKGROUND: Expired breath condensate (EBC) has never been used to explore the level of oxidative stress in idiopathic pulmonary fibrosis (IPF). Therefore, the aim of this study was to measure the levels of H2O2 and 8-isoprostane, as biomarkers of oxidative stress, in the EBC of patients with IPF. MATERIALS AND METHODS: We investigated 16 patients with IPF and 15 healthy subjects as the control group. The levels of H2O2 and 8-isoprostane were measured in the EBC of all subjects and were compared between the IPF and control groups. In patients with IPF, H2O2 and 8-isoprostane were further correlated with pulmonary function tests (PFTs), the resting pO2 and the differential cell count from the bronchoalveolar lavage fluid (BALF). RESULTS: The mean (95%CI) concentration of H2O2 was increased in the patients with IPF compared with the normal subjects (0.36, 0.24-0.47 microM vs. 0.16, 0.10-0.23 microM, P=0.003). The mean (95%CI) concentration of 8-isoprostane was also increased in the patients with IPF compared with the controls (74, 38-110 pg mL-1 vs. 33, 28-39 pg mL-1, P=0.02). In the patients with IPF, the diffusing capacity of the lung for carbon monoxide was negatively correlated with the levels of H2O2 in EBC (P=0.03, r=-0.58). No other correlation was found between the oxidative stress markers in the EBC and PFT values, pO2 or BALF cell count. CONCLUSIONS: Our data suggest that H2O2 and 8-isoprostane are increased in the EBC of patients with IPF. H2O2 may be correlated with the severity of the disease in IPF. SN - 0014-2972 UR - https://www.unboundmedicine.com/medline/citation/16634841/Exhaled_markers_of_oxidative_stress_in_idiopathic_pulmonary_fibrosis_ L2 - https://doi.org/10.1111/j.1365-2362.2006.01636.x DB - PRIME DP - Unbound Medicine ER -