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Peptide profiling by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of the Lasiodora parahybana tarantula venom gland.
Toxicon. 2006 May; 47(6):640-9.T

Abstract

In order to establish a venom fingerprint and a peptide profile of the Lasiodora parahybana tarantula venom gland, we used conventional methods such as reversed phase liquid chromatography coupled to an electrospray-ionisation hybrid quadrupole time of flight mass spectrometer (LC/ESI-QqTOFMS), matrix-assisted laser desorption/ionization time-of-flight-MS (MALDI-TOFMS) and direct study of L. parahybana venom by nanospray-ionization QqTOFMS (nanoESI-QqTOFMS) and a new technology for the direct analysis of fresh tissues using MALDI-TOFMS. The analysis of the crude venom allowed the characterization of specific juvenile and adult biomarkers. In situ MALDI analysis of L. parahybana venom gland sections revealed different peptide expression levels all along the gland and non-processed peptide precursors, demonstrating the power of the method for the dynamic investigation of peptide evolution in the venom gland of spiders.

Authors+Show Affiliations

USM 0505, Ecosystèmes et Interactions Toxiques, Muséum National d'Histoire Naturelle, 18 rue Buffon, 75231 Paris Cedex 05, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16635501

Citation

Guette, Catherine, et al. "Peptide Profiling By Matrix-assisted Laser Desorption/ionisation Time-of-flight Mass Spectrometry of the Lasiodora Parahybana Tarantula Venom Gland." Toxicon : Official Journal of the International Society On Toxinology, vol. 47, no. 6, 2006, pp. 640-9.
Guette C, Legros C, Tournois G, et al. Peptide profiling by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of the Lasiodora parahybana tarantula venom gland. Toxicon. 2006;47(6):640-9.
Guette, C., Legros, C., Tournois, G., Goyffon, M., & Célérier, M. L. (2006). Peptide profiling by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of the Lasiodora parahybana tarantula venom gland. Toxicon : Official Journal of the International Society On Toxinology, 47(6), 640-9.
Guette C, et al. Peptide Profiling By Matrix-assisted Laser Desorption/ionisation Time-of-flight Mass Spectrometry of the Lasiodora Parahybana Tarantula Venom Gland. Toxicon. 2006;47(6):640-9. PubMed PMID: 16635501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peptide profiling by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of the Lasiodora parahybana tarantula venom gland. AU - Guette,Catherine, AU - Legros,Christian, AU - Tournois,Guewen, AU - Goyffon,Max, AU - Célérier,Marie-Louise, Y1 - 2006/04/25/ PY - 2006/4/26/pubmed PY - 2006/6/30/medline PY - 2006/4/26/entrez SP - 640 EP - 9 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 47 IS - 6 N2 - In order to establish a venom fingerprint and a peptide profile of the Lasiodora parahybana tarantula venom gland, we used conventional methods such as reversed phase liquid chromatography coupled to an electrospray-ionisation hybrid quadrupole time of flight mass spectrometer (LC/ESI-QqTOFMS), matrix-assisted laser desorption/ionization time-of-flight-MS (MALDI-TOFMS) and direct study of L. parahybana venom by nanospray-ionization QqTOFMS (nanoESI-QqTOFMS) and a new technology for the direct analysis of fresh tissues using MALDI-TOFMS. The analysis of the crude venom allowed the characterization of specific juvenile and adult biomarkers. In situ MALDI analysis of L. parahybana venom gland sections revealed different peptide expression levels all along the gland and non-processed peptide precursors, demonstrating the power of the method for the dynamic investigation of peptide evolution in the venom gland of spiders. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/16635501/Peptide_profiling_by_matrix_assisted_laser_desorption/ionisation_time_of_flight_mass_spectrometry_of_the_Lasiodora_parahybana_tarantula_venom_gland_ DB - PRIME DP - Unbound Medicine ER -