Tags

Type your tag names separated by a space and hit enter

Dissociation of biomolecules using a ultraviolet matrix-assisted laser desorption/ionisation time-of-flight/curved field reflectron tandem mass spectrometer equipped with a differential-pumped collision cell.
Rapid Commun Mass Spectrom. 2006; 20(11):1653-60.RC

Abstract

A commercial matrix-assisted laser desorption/ionisation time-of-flight (MALDI-ToF) instrument equipped with a curved field reflectron (CFR) was modified in order to perform collision-induced dissociation (CID) on a variety of biomolecules. The incorporation of a high-resolution ion gate together with a collision cell within the field-free region allowed tandem mass analysis (MS/MS), without the necessity to decelerate the precursor ions prior to activation. The simultaneous detection of all product ions remained possible by using the CFR. To test the MS/MS performances, ACTH (fragment 1-17), a complex high mannose carbohydrate (Man)(8)(GlcNac)(2) and a lysophosphatidylcholine lipid (18:1) were analysed on the modified instrument. Direct comparison with the low-energy product ion spectra, acquired on a MALDI quadrupole ion trap (QIT) two-stage reflectron time-of flight (ReToF) mass spectrometer, showed significant differences in the types of product ions observed. The additional ions detected were a clear indication of the high-energy fragmentation processes occurring in the collision cell.

Authors+Show Affiliations

Shimadzu Biotech, Wharfside, Trafford Wharf Road, Manchester M17 1GP, UK. Omar.Belacem@kratos.co.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16636997

Citation

Belgacem, Omar, et al. "Dissociation of Biomolecules Using a Ultraviolet Matrix-assisted Laser Desorption/ionisation Time-of-flight/curved Field Reflectron Tandem Mass Spectrometer Equipped With a Differential-pumped Collision Cell." Rapid Communications in Mass Spectrometry : RCM, vol. 20, no. 11, 2006, pp. 1653-60.
Belgacem O, Bowdler A, Brookhouse I, et al. Dissociation of biomolecules using a ultraviolet matrix-assisted laser desorption/ionisation time-of-flight/curved field reflectron tandem mass spectrometer equipped with a differential-pumped collision cell. Rapid Commun Mass Spectrom. 2006;20(11):1653-60.
Belgacem, O., Bowdler, A., Brookhouse, I., Brancia, F. L., & Raptakis, E. (2006). Dissociation of biomolecules using a ultraviolet matrix-assisted laser desorption/ionisation time-of-flight/curved field reflectron tandem mass spectrometer equipped with a differential-pumped collision cell. Rapid Communications in Mass Spectrometry : RCM, 20(11), 1653-60.
Belgacem O, et al. Dissociation of Biomolecules Using a Ultraviolet Matrix-assisted Laser Desorption/ionisation Time-of-flight/curved Field Reflectron Tandem Mass Spectrometer Equipped With a Differential-pumped Collision Cell. Rapid Commun Mass Spectrom. 2006;20(11):1653-60. PubMed PMID: 16636997.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissociation of biomolecules using a ultraviolet matrix-assisted laser desorption/ionisation time-of-flight/curved field reflectron tandem mass spectrometer equipped with a differential-pumped collision cell. AU - Belgacem,Omar, AU - Bowdler,Andrew, AU - Brookhouse,Ian, AU - Brancia,Francesco L, AU - Raptakis,Emmanuel, PY - 2006/4/26/pubmed PY - 2007/8/19/medline PY - 2006/4/26/entrez SP - 1653 EP - 60 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 20 IS - 11 N2 - A commercial matrix-assisted laser desorption/ionisation time-of-flight (MALDI-ToF) instrument equipped with a curved field reflectron (CFR) was modified in order to perform collision-induced dissociation (CID) on a variety of biomolecules. The incorporation of a high-resolution ion gate together with a collision cell within the field-free region allowed tandem mass analysis (MS/MS), without the necessity to decelerate the precursor ions prior to activation. The simultaneous detection of all product ions remained possible by using the CFR. To test the MS/MS performances, ACTH (fragment 1-17), a complex high mannose carbohydrate (Man)(8)(GlcNac)(2) and a lysophosphatidylcholine lipid (18:1) were analysed on the modified instrument. Direct comparison with the low-energy product ion spectra, acquired on a MALDI quadrupole ion trap (QIT) two-stage reflectron time-of flight (ReToF) mass spectrometer, showed significant differences in the types of product ions observed. The additional ions detected were a clear indication of the high-energy fragmentation processes occurring in the collision cell. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/16636997/Dissociation_of_biomolecules_using_a_ultraviolet_matrix_assisted_laser_desorption/ionisation_time_of_flight/curved_field_reflectron_tandem_mass_spectrometer_equipped_with_a_differential_pumped_collision_cell_ L2 - https://doi.org/10.1002/rcm.2486 DB - PRIME DP - Unbound Medicine ER -