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Quantification of clopidogrel in human plasma by sensitive liquid chromatography/tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2006; 20(11):1695-700.RC

Abstract

A simple, sensitive and rapid high-performance liquid chromatography/positive electrospray ionization tandem mass spectrometry method was developed and validated for the assay of clopidogrel in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed-phase column and analyzed by mass spectrometry in the multiple reaction monitoring mode using the respective [M+H](+) ions, m/z 322/212 for clopidogrel and m/z 264/154 for the internal standard. The assay exhibited a linear dynamic range of 5-6000 pg/mL for clopidogrel in human plasma. The lower limit of quantification was 5 pg/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.5 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.

Authors+Show Affiliations

Biopharmaceutical Research, Suven Life Sciences Ltd., Serene Chambers, Road # 7, Banjara Hills, Hyderabad 500 034, India. ramakrishna_nirogi@yahoo.co.inNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16637000

Citation

Nirogi, Ramakrishna V S., et al. "Quantification of Clopidogrel in Human Plasma By Sensitive Liquid Chromatography/tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 20, no. 11, 2006, pp. 1695-700.
Nirogi RV, Kandikere VN, Shukla M, et al. Quantification of clopidogrel in human plasma by sensitive liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom. 2006;20(11):1695-700.
Nirogi, R. V., Kandikere, V. N., Shukla, M., Mudigonda, K., Maurya, S., & Boosi, R. (2006). Quantification of clopidogrel in human plasma by sensitive liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 20(11), 1695-700.
Nirogi RV, et al. Quantification of Clopidogrel in Human Plasma By Sensitive Liquid Chromatography/tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2006;20(11):1695-700. PubMed PMID: 16637000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of clopidogrel in human plasma by sensitive liquid chromatography/tandem mass spectrometry. AU - Nirogi,Ramakrishna V S, AU - Kandikere,Vishwottam N, AU - Shukla,Manoj, AU - Mudigonda,Koteshwara, AU - Maurya,Santosh, AU - Boosi,Ravikumar, PY - 2006/4/26/pubmed PY - 2007/8/19/medline PY - 2006/4/26/entrez SP - 1695 EP - 700 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 20 IS - 11 N2 - A simple, sensitive and rapid high-performance liquid chromatography/positive electrospray ionization tandem mass spectrometry method was developed and validated for the assay of clopidogrel in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed-phase column and analyzed by mass spectrometry in the multiple reaction monitoring mode using the respective [M+H](+) ions, m/z 322/212 for clopidogrel and m/z 264/154 for the internal standard. The assay exhibited a linear dynamic range of 5-6000 pg/mL for clopidogrel in human plasma. The lower limit of quantification was 5 pg/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.5 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/16637000/Quantification_of_clopidogrel_in_human_plasma_by_sensitive_liquid_chromatography/tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.2497 DB - PRIME DP - Unbound Medicine ER -