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Determination of the predominant catechins in Acacia catechu by liquid chromatography/electrospray ionization-mass spectrometry.
J Agric Food Chem. 2006 May 03; 54(9):3219-24.JA

Abstract

A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI-MS) method under selected ion monitoring mode (SIM) was developed to quantitate the predominant catechins, catechin, epicatechin, epicatechin-3-O-gallate, and epigallocatechin-3-O-gallate, in the medicinal plant catechu (Acacia catechu). Other major secondary products including caffeine, flavanol dimers, and flavonol glycosides were also identified by their molecular ion peaks and fragmentation peaks using LC/MS and LC/MS/MS. For the investigated ion concentration ranges of catechin, epicatechin, epicatechin-3-O-gallate, and epigallocatechin-3-O-gallate, good linearities (r2 > 0.99) were obtained for each calibration curve. Validation for this method showed an accuracy ranging from 1.06 to 11.76%, and the precision (relative standard deviation) varied between 1.60 and 9.36% for these four analytes. This is the first quantitative determination of all predominant catechins in catechu heartwood and leaves.

Authors+Show Affiliations

Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey 08854, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16637676

Citation

Shen, Diandian, et al. "Determination of the Predominant Catechins in Acacia Catechu By Liquid Chromatography/electrospray Ionization-mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 54, no. 9, 2006, pp. 3219-24.
Shen D, Wu Q, Wang M, et al. Determination of the predominant catechins in Acacia catechu by liquid chromatography/electrospray ionization-mass spectrometry. J Agric Food Chem. 2006;54(9):3219-24.
Shen, D., Wu, Q., Wang, M., Yang, Y., Lavoie, E. J., & Simon, J. E. (2006). Determination of the predominant catechins in Acacia catechu by liquid chromatography/electrospray ionization-mass spectrometry. Journal of Agricultural and Food Chemistry, 54(9), 3219-24.
Shen D, et al. Determination of the Predominant Catechins in Acacia Catechu By Liquid Chromatography/electrospray Ionization-mass Spectrometry. J Agric Food Chem. 2006 May 3;54(9):3219-24. PubMed PMID: 16637676.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of the predominant catechins in Acacia catechu by liquid chromatography/electrospray ionization-mass spectrometry. AU - Shen,Diandian, AU - Wu,Qingli, AU - Wang,Mingfu, AU - Yang,Yonghong, AU - Lavoie,Edmond J, AU - Simon,James E, PY - 2006/4/28/pubmed PY - 2006/6/21/medline PY - 2006/4/28/entrez SP - 3219 EP - 24 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 9 N2 - A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI-MS) method under selected ion monitoring mode (SIM) was developed to quantitate the predominant catechins, catechin, epicatechin, epicatechin-3-O-gallate, and epigallocatechin-3-O-gallate, in the medicinal plant catechu (Acacia catechu). Other major secondary products including caffeine, flavanol dimers, and flavonol glycosides were also identified by their molecular ion peaks and fragmentation peaks using LC/MS and LC/MS/MS. For the investigated ion concentration ranges of catechin, epicatechin, epicatechin-3-O-gallate, and epigallocatechin-3-O-gallate, good linearities (r2 > 0.99) were obtained for each calibration curve. Validation for this method showed an accuracy ranging from 1.06 to 11.76%, and the precision (relative standard deviation) varied between 1.60 and 9.36% for these four analytes. This is the first quantitative determination of all predominant catechins in catechu heartwood and leaves. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16637676/Determination_of_the_predominant_catechins_in_Acacia_catechu_by_liquid_chromatography/electrospray_ionization_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -