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Urinary activities of cathepsin B, N-acetyl-beta-D-glucosaminidase, and albuminuria in patients with type 2 diabetes mellitus.
Med Sci Monit 2006; 12(5):CR210-4MS

Abstract

BACKGROUND

The proximal kidney tubule contains a large number of lysosomes involved in the breakdown of intracellular as well as reabsorbed proteins. When tubular protein reabsorption is overburdened or when there is tubular cell damage, higher urinary excretion of lysosomal enzymes, e.g. cathepsins and N-acetyl-beta-D-glucosaminidase (NAG), can be found. We compared urinary cathepsin B (CB) activity with NAG in diabetic patients.

MATERIAL/METHODS

Using fluorogenic substrates, urinary and plasma CB and NAG activities in 130 type 2 diabetic patients with varying stages of albuminuria and 42 control subjects were determined. Early morning urine samples were used.

RESULTS

In the patients, only higher values of plasma NAG were found. In urine, CB and NAG activities increased progressively from normoalbuminuria, through microalbuminuria to macroalbuminuria group. The normoalbuminuria group had both enzyme activities higher than healthy controls. Urine CB activity in the patients also increased gradually to tertiles of urinary NAG. Only urinary CB activity was significantly associated with glycemic state. The correlation was stronger in the patients with poor glycemic control. The plasma/urine ratios for both CB and NAG decreased in the patients compared with controls.

CONCLUSIONS

Determination of urinary CB activity might be useful as a non-invasive surrogate marker of incipient nephropathy.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry of the Wrocław Medical University, Wrocław, Poland. piwowar@bf.uni.wroc.plNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16641878

Citation

Piwowar, Agnieszka, et al. "Urinary Activities of Cathepsin B, N-acetyl-beta-D-glucosaminidase, and Albuminuria in Patients With Type 2 Diabetes Mellitus." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 12, no. 5, 2006, pp. CR210-4.
Piwowar A, Knapik-Kordecka M, Fus I, et al. Urinary activities of cathepsin B, N-acetyl-beta-D-glucosaminidase, and albuminuria in patients with type 2 diabetes mellitus. Med Sci Monit. 2006;12(5):CR210-4.
Piwowar, A., Knapik-Kordecka, M., Fus, I., & Warwas, M. (2006). Urinary activities of cathepsin B, N-acetyl-beta-D-glucosaminidase, and albuminuria in patients with type 2 diabetes mellitus. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 12(5), pp. CR210-4.
Piwowar A, et al. Urinary Activities of Cathepsin B, N-acetyl-beta-D-glucosaminidase, and Albuminuria in Patients With Type 2 Diabetes Mellitus. Med Sci Monit. 2006;12(5):CR210-4. PubMed PMID: 16641878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urinary activities of cathepsin B, N-acetyl-beta-D-glucosaminidase, and albuminuria in patients with type 2 diabetes mellitus. AU - Piwowar,Agnieszka, AU - Knapik-Kordecka,Maria, AU - Fus,Izabela, AU - Warwas,Maria, PY - 2003/06/24/received PY - 2004/04/27/accepted PY - 2006/4/28/pubmed PY - 2006/9/9/medline PY - 2006/4/28/entrez SP - CR210 EP - 4 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med. Sci. Monit. VL - 12 IS - 5 N2 - BACKGROUND: The proximal kidney tubule contains a large number of lysosomes involved in the breakdown of intracellular as well as reabsorbed proteins. When tubular protein reabsorption is overburdened or when there is tubular cell damage, higher urinary excretion of lysosomal enzymes, e.g. cathepsins and N-acetyl-beta-D-glucosaminidase (NAG), can be found. We compared urinary cathepsin B (CB) activity with NAG in diabetic patients. MATERIAL/METHODS: Using fluorogenic substrates, urinary and plasma CB and NAG activities in 130 type 2 diabetic patients with varying stages of albuminuria and 42 control subjects were determined. Early morning urine samples were used. RESULTS: In the patients, only higher values of plasma NAG were found. In urine, CB and NAG activities increased progressively from normoalbuminuria, through microalbuminuria to macroalbuminuria group. The normoalbuminuria group had both enzyme activities higher than healthy controls. Urine CB activity in the patients also increased gradually to tertiles of urinary NAG. Only urinary CB activity was significantly associated with glycemic state. The correlation was stronger in the patients with poor glycemic control. The plasma/urine ratios for both CB and NAG decreased in the patients compared with controls. CONCLUSIONS: Determination of urinary CB activity might be useful as a non-invasive surrogate marker of incipient nephropathy. SN - 1234-1010 UR - https://www.unboundmedicine.com/medline/citation/16641878/Urinary_activities_of_cathepsin_B_N_acetyl_beta_D_glucosaminidase_and_albuminuria_in_patients_with_type_2_diabetes_mellitus_ L2 - https://www.medscimonit.com/download/index/idArt/450290 DB - PRIME DP - Unbound Medicine ER -