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    [Effects of Shuanggu Yitong needling method on proliferation and differentiation of nerve stem cells in the Parkinson's disease model rat].

    Abstract

    OBJECTIVE

    To explore the mechanism of acupuncture in treatment of Parkinson's disease.

    METHODS

    Fifty Wistar rats were randomly divided into a normal control group, a sham-operation control group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. The hemilateral rotation Parkinson's disease model was prepared by micro-injection of 6-hydroxyl-dopamine into the right striatum. Effects of acupuncture at "Fengfu" (GV 16), "Taichong" (LR 3) hy Shoanggu Yitong needling method on proliferation and differentiation of nerve stem cells in the Parkinson's disease model rat were compared. Proliferation and numher changes of the nerve stem cells in the suhstantia nigra and the striatum were investigated with immunohistochemical method, and the number of nerve stem cells transforming neurons were observed with immunohistochemical double-labeling method.

    RESULTS

    In the two acupuncture groups, the proliferative cells in the substantia nigra and thestriatum of the destroyed side increased significantly (P < 0.01), and the number of nerve stem cells and the number of transformed neurons in the destroyed side of the substantia nigra and the striatum increased significantly only in the Shuanggu Yitong group (P < 0.01).

    CONCLUSION

    Shuanggu Yitong needling method can induce proliferation and differentiation of nerve stem cells in the substantia nigra and the striatum regions in the Parkinson's disease model rat.

    Authors+Show Affiliations

    Wang YC
    ,

    Department of Acup-Moxibustion, Orthopedics and Traumatology, Wuhan 430061, China. wangyanchunonly@yahoo.com.cn

    Ma J
    ,
    Wang H

    Source

    MeSH

    Acupuncture Therapy
    Animals
    Bromodeoxyuridine
    Cell Differentiation
    Cell Proliferation
    Corpus Striatum
    Male
    Neurons
    Parkinson Disease
    Rats
    Rats, Wistar
    Stem Cells
    Substantia Nigra

    Pub Type(s)

    English Abstract
    Journal Article

    Language

    chi

    PubMed ID

    16642616

    Citation

    TY - JOUR T1 - [Effects of Shuanggu Yitong needling method on proliferation and differentiation of nerve stem cells in the Parkinson's disease model rat]. AU - Wang,Yan-chun, AU - Ma,Jun, AU - Wang,Hua, PY - 2006/4/29/pubmed PY - 2006/5/23/medline PY - 2006/4/29/entrez SP - 277 EP - 82 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 26 IS - 4 N2 - OBJECTIVE: To explore the mechanism of acupuncture in treatment of Parkinson's disease. METHODS: Fifty Wistar rats were randomly divided into a normal control group, a sham-operation control group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. The hemilateral rotation Parkinson's disease model was prepared by micro-injection of 6-hydroxyl-dopamine into the right striatum. Effects of acupuncture at "Fengfu" (GV 16), "Taichong" (LR 3) hy Shoanggu Yitong needling method on proliferation and differentiation of nerve stem cells in the Parkinson's disease model rat were compared. Proliferation and numher changes of the nerve stem cells in the suhstantia nigra and the striatum were investigated with immunohistochemical method, and the number of nerve stem cells transforming neurons were observed with immunohistochemical double-labeling method. RESULTS: In the two acupuncture groups, the proliferative cells in the substantia nigra and thestriatum of the destroyed side increased significantly (P < 0.01), and the number of nerve stem cells and the number of transformed neurons in the destroyed side of the substantia nigra and the striatum increased significantly only in the Shuanggu Yitong group (P < 0.01). CONCLUSION: Shuanggu Yitong needling method can induce proliferation and differentiation of nerve stem cells in the substantia nigra and the striatum regions in the Parkinson's disease model rat. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/16642616/[Effects_of_Shuanggu_Yitong_needling_method_on_proliferation_and_differentiation_of_nerve_stem_cells_in_the_Parkinson's_disease_model_rat]_ L2 - https://medlineplus.gov/parkinsonsdisease.html ER -