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Comparison of amperometric biosensors fabricated by palladium sputtering, palladium electrodeposition and Nafion/carbon nanotube casting on screen-printed carbon electrodes.
Biosens Bioelectron. 2007 Jan 15; 22(6):877-84.BB

Abstract

Different strategies, including palladium electrodeposition (Pd(CV)), Pd sputtering (Pd(S)) and Nafion-solubilized carbon nanotube casting (Nafion/CNT), were used to modify screen-printed carbon electrodes (SPCEs) for the fabrication of amperometric enzyme biosensors. The electrochemical properties of the bare and modified SPCEs and the optimal conditions for surface modification were determined. The electrochemical response of the bare SPCE to H(2)O(2) under the potential of 0.3 V could be improved about 100-fold by Pd modification by electrodeposition or sputtering. By contrast, the electrochemical response of the bare SPCE was enhanced by only about 11-fold by Nafion/CNT casting. Moreover, the Pd(CV)-SPCEs exhibited better reproducibility of electrochemical response (a relative standard deviation (R.S.D.)<6.0%) than freshly prepared Pd(S)-SPCEs (R.S.D.>10%). The glucose biosensor fabricated from Pd-modified electrodes could be stored for up to 108 days without loosing significant activity. The Pd(CV)-SPCE also showed very reliable signal characteristics upon 50 consecutively repeated measurements of ascorbic acid. The electrocatalytic detection of the Pd-SPCE was combined with additional advantages of resistance to surface fouling and hence good stability. In conclusion, this study demonstrated that deposition of Pd thin film on SPCEs by electrodeposition or sputtering provided superior enhancement of electrochemical properties compared to Nafion/CNT-SPCEs. Despite their high electrochemical response, Pd(S)-SPCEs required an activation process to improve stability and Pd(CV)-SPCEs suffered from poor between electrode reproducibility.

Authors+Show Affiliations

Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16644200

Citation

Lee, Chung-Hun, et al. "Comparison of Amperometric Biosensors Fabricated By Palladium Sputtering, Palladium Electrodeposition and Nafion/carbon Nanotube Casting On Screen-printed Carbon Electrodes." Biosensors & Bioelectronics, vol. 22, no. 6, 2007, pp. 877-84.
Lee CH, Wang SC, Yuan CJ, et al. Comparison of amperometric biosensors fabricated by palladium sputtering, palladium electrodeposition and Nafion/carbon nanotube casting on screen-printed carbon electrodes. Biosens Bioelectron. 2007;22(6):877-84.
Lee, C. H., Wang, S. C., Yuan, C. J., Wen, M. F., & Chang, K. S. (2007). Comparison of amperometric biosensors fabricated by palladium sputtering, palladium electrodeposition and Nafion/carbon nanotube casting on screen-printed carbon electrodes. Biosensors & Bioelectronics, 22(6), 877-84.
Lee CH, et al. Comparison of Amperometric Biosensors Fabricated By Palladium Sputtering, Palladium Electrodeposition and Nafion/carbon Nanotube Casting On Screen-printed Carbon Electrodes. Biosens Bioelectron. 2007 Jan 15;22(6):877-84. PubMed PMID: 16644200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of amperometric biosensors fabricated by palladium sputtering, palladium electrodeposition and Nafion/carbon nanotube casting on screen-printed carbon electrodes. AU - Lee,Chung-Hun, AU - Wang,Shih-Chang, AU - Yuan,Chiun-Jye, AU - Wen,Meng-Fang, AU - Chang,Ku-Shang, Y1 - 2006/04/27/ PY - 2005/12/06/received PY - 2006/02/10/revised PY - 2006/03/03/accepted PY - 2006/4/29/pubmed PY - 2007/3/7/medline PY - 2006/4/29/entrez SP - 877 EP - 84 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 6 N2 - Different strategies, including palladium electrodeposition (Pd(CV)), Pd sputtering (Pd(S)) and Nafion-solubilized carbon nanotube casting (Nafion/CNT), were used to modify screen-printed carbon electrodes (SPCEs) for the fabrication of amperometric enzyme biosensors. The electrochemical properties of the bare and modified SPCEs and the optimal conditions for surface modification were determined. The electrochemical response of the bare SPCE to H(2)O(2) under the potential of 0.3 V could be improved about 100-fold by Pd modification by electrodeposition or sputtering. By contrast, the electrochemical response of the bare SPCE was enhanced by only about 11-fold by Nafion/CNT casting. Moreover, the Pd(CV)-SPCEs exhibited better reproducibility of electrochemical response (a relative standard deviation (R.S.D.)<6.0%) than freshly prepared Pd(S)-SPCEs (R.S.D.>10%). The glucose biosensor fabricated from Pd-modified electrodes could be stored for up to 108 days without loosing significant activity. The Pd(CV)-SPCE also showed very reliable signal characteristics upon 50 consecutively repeated measurements of ascorbic acid. The electrocatalytic detection of the Pd-SPCE was combined with additional advantages of resistance to surface fouling and hence good stability. In conclusion, this study demonstrated that deposition of Pd thin film on SPCEs by electrodeposition or sputtering provided superior enhancement of electrochemical properties compared to Nafion/CNT-SPCEs. Despite their high electrochemical response, Pd(S)-SPCEs required an activation process to improve stability and Pd(CV)-SPCEs suffered from poor between electrode reproducibility. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16644200/Comparison_of_amperometric_biosensors_fabricated_by_palladium_sputtering_palladium_electrodeposition_and_Nafion/carbon_nanotube_casting_on_screen_printed_carbon_electrodes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00096-0 DB - PRIME DP - Unbound Medicine ER -