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Comparative evaluation of genotoxicity of captan in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test.
Environ Toxicol. 2006 Jun; 21(3):264-77.ET

Abstract

Captan (N-trichloromethylthio-4-cyclohexene-1,2-dicarboximide) is a fungicide used to inhibit the growth of many types of fungi on plants used as foodstuffs. The toxic and genotoxic potentials of captan were evaluated with the micronucleus test (MNT; AFNOR,2000) and the comet assay (CA) using amphibian larvae (Xenopus laevis and Pleurodeles waltl). Acute toxicity results showed that captan was toxic (1) to Xenopus larvae exposed to from 2 mg/L to 125 or 62.5 microg/L, depending on the nature of the water [reconstituted water containing mineral salts or mineral water (MW; Volvic, Danone, France)] and (2) to Pleurodeles exposed to from 2 mg/L to 125 microg/L in both types of water. The MNT results obtained in MW showed that captan (62.5 microg/L) was genotoxic to Xenopus but not genotoxic to Pleurodeles at all concentrations tested. CA established that the genotoxicity of captan to Xenopus and Pleurodeles larvae depended on the concentration, the exposure times, and the comet parameters (tail DNA, TEM, OTM, and TL). The CA and MNT results were compared for their ability to detect DNA damage at the concentrations of captan and the exposure times applied. CA showed captan to be genotoxic from the first day of exposure. In amphibians, CA appears to be a sensitive and suitable method for detecting genotoxicity such as that caused by captan.

Authors+Show Affiliations

Laboratoire d'Ecologie des Hydrosystèmes--LEH, UMR CNRS UPS 5177, 29 Rue Jeanne Marvig, 31055 Toulouse, France. florence.mouchet@wanadoo.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16646022

Citation

Mouchet, F, et al. "Comparative Evaluation of Genotoxicity of Captan in Amphibian Larvae (Xenopus Laevis and Pleurodeles Waltl) Using the Comet Assay and the Micronucleus Test." Environmental Toxicology, vol. 21, no. 3, 2006, pp. 264-77.
Mouchet F, Gauthier L, Mailhes C, et al. Comparative evaluation of genotoxicity of captan in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test. Environ Toxicol. 2006;21(3):264-77.
Mouchet, F., Gauthier, L., Mailhes, C., Ferrier, V., & Devaux, A. (2006). Comparative evaluation of genotoxicity of captan in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test. Environmental Toxicology, 21(3), 264-77.
Mouchet F, et al. Comparative Evaluation of Genotoxicity of Captan in Amphibian Larvae (Xenopus Laevis and Pleurodeles Waltl) Using the Comet Assay and the Micronucleus Test. Environ Toxicol. 2006;21(3):264-77. PubMed PMID: 16646022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative evaluation of genotoxicity of captan in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test. AU - Mouchet,F, AU - Gauthier,L, AU - Mailhes,C, AU - Ferrier,V, AU - Devaux,A, PY - 2006/4/29/pubmed PY - 2006/9/30/medline PY - 2006/4/29/entrez SP - 264 EP - 77 JF - Environmental toxicology JO - Environ Toxicol VL - 21 IS - 3 N2 - Captan (N-trichloromethylthio-4-cyclohexene-1,2-dicarboximide) is a fungicide used to inhibit the growth of many types of fungi on plants used as foodstuffs. The toxic and genotoxic potentials of captan were evaluated with the micronucleus test (MNT; AFNOR,2000) and the comet assay (CA) using amphibian larvae (Xenopus laevis and Pleurodeles waltl). Acute toxicity results showed that captan was toxic (1) to Xenopus larvae exposed to from 2 mg/L to 125 or 62.5 microg/L, depending on the nature of the water [reconstituted water containing mineral salts or mineral water (MW; Volvic, Danone, France)] and (2) to Pleurodeles exposed to from 2 mg/L to 125 microg/L in both types of water. The MNT results obtained in MW showed that captan (62.5 microg/L) was genotoxic to Xenopus but not genotoxic to Pleurodeles at all concentrations tested. CA established that the genotoxicity of captan to Xenopus and Pleurodeles larvae depended on the concentration, the exposure times, and the comet parameters (tail DNA, TEM, OTM, and TL). The CA and MNT results were compared for their ability to detect DNA damage at the concentrations of captan and the exposure times applied. CA showed captan to be genotoxic from the first day of exposure. In amphibians, CA appears to be a sensitive and suitable method for detecting genotoxicity such as that caused by captan. SN - 1520-4081 UR - https://www.unboundmedicine.com/medline/citation/16646022/Comparative_evaluation_of_genotoxicity_of_captan_in_amphibian_larvae__Xenopus_laevis_and_Pleurodeles_waltl__using_the_comet_assay_and_the_micronucleus_test_ L2 - https://doi.org/10.1002/tox.20180 DB - PRIME DP - Unbound Medicine ER -