Tags

Type your tag names separated by a space and hit enter

Carbon monoxide protects hepatocytes from TNF-alpha/Actinomycin D by inhibition of the caspase-8-mediated apoptotic pathway.
Biochem Biophys Res Commun 2006; 344(4):1172-8BB

Abstract

We have previously shown that carbon monoxide (CO) (250 ppm) prevented tumor necrosis factor-alpha (TNFalpha)-induced apoptosis and activated the transcription factor NF-kappaB in hepatocytes both in vivo and in vitro. These studies were conducted to further determine the mechanisms by which CO suppresses apoptotic signaling in TNFalpha (10 ng/ml) and Actinomycin D (ActD, 200 ng/ml)-treated hepatocytes. Consistent with our previous findings, CO protected against TNFalpha/ActD-induced cell death, which is in part dependent on NF-kappaB activation. This was associated with a reduction in mitochondrial damage, a decrease in cytochrome c release, and an inhibition of translocation of Bcl proteins to mitochondria. In conjugation with inhibition of these mitochondrial events, CO also suppressed caspases-8 and -3 cleavage in response to TNFalpha/ActD. Inhibition of NF-kappaB activation resulted in diminished CO-induced cFLIP expression and increased caspase-8 cleavage from cells treated with TNFalpha/ActD. These data indicate that CO interferes with apoptotic signaling at a proximal step.

Authors+Show Affiliations

Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16647044

Citation

Kim, Hoe Suk, et al. "Carbon Monoxide Protects Hepatocytes From TNF-alpha/Actinomycin D By Inhibition of the Caspase-8-mediated Apoptotic Pathway." Biochemical and Biophysical Research Communications, vol. 344, no. 4, 2006, pp. 1172-8.
Kim HS, Loughran PA, Kim PK, et al. Carbon monoxide protects hepatocytes from TNF-alpha/Actinomycin D by inhibition of the caspase-8-mediated apoptotic pathway. Biochem Biophys Res Commun. 2006;344(4):1172-8.
Kim, H. S., Loughran, P. A., Kim, P. K., Billiar, T. R., & Zuckerbraun, B. S. (2006). Carbon monoxide protects hepatocytes from TNF-alpha/Actinomycin D by inhibition of the caspase-8-mediated apoptotic pathway. Biochemical and Biophysical Research Communications, 344(4), pp. 1172-8.
Kim HS, et al. Carbon Monoxide Protects Hepatocytes From TNF-alpha/Actinomycin D By Inhibition of the Caspase-8-mediated Apoptotic Pathway. Biochem Biophys Res Commun. 2006 Jun 16;344(4):1172-8. PubMed PMID: 16647044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbon monoxide protects hepatocytes from TNF-alpha/Actinomycin D by inhibition of the caspase-8-mediated apoptotic pathway. AU - Kim,Hoe Suk, AU - Loughran,Patricia A, AU - Kim,Peter K, AU - Billiar,Timothy R, AU - Zuckerbraun,Brian S, Y1 - 2006/04/06/ PY - 2006/03/22/received PY - 2006/03/24/accepted PY - 2006/5/2/pubmed PY - 2006/7/20/medline PY - 2006/5/2/entrez SP - 1172 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 344 IS - 4 N2 - We have previously shown that carbon monoxide (CO) (250 ppm) prevented tumor necrosis factor-alpha (TNFalpha)-induced apoptosis and activated the transcription factor NF-kappaB in hepatocytes both in vivo and in vitro. These studies were conducted to further determine the mechanisms by which CO suppresses apoptotic signaling in TNFalpha (10 ng/ml) and Actinomycin D (ActD, 200 ng/ml)-treated hepatocytes. Consistent with our previous findings, CO protected against TNFalpha/ActD-induced cell death, which is in part dependent on NF-kappaB activation. This was associated with a reduction in mitochondrial damage, a decrease in cytochrome c release, and an inhibition of translocation of Bcl proteins to mitochondria. In conjugation with inhibition of these mitochondrial events, CO also suppressed caspases-8 and -3 cleavage in response to TNFalpha/ActD. Inhibition of NF-kappaB activation resulted in diminished CO-induced cFLIP expression and increased caspase-8 cleavage from cells treated with TNFalpha/ActD. These data indicate that CO interferes with apoptotic signaling at a proximal step. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/16647044/Carbon_monoxide_protects_hepatocytes_from_TNF_alpha/Actinomycin_D_by_inhibition_of_the_caspase_8_mediated_apoptotic_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(06)00713-3 DB - PRIME DP - Unbound Medicine ER -