Citation
Gauna, Carlotta, et al. "Unacylated Ghrelin Is Active On the INS-1E Rat Insulinoma Cell Line Independently of the Growth Hormone Secretagogue Receptor Type 1a and the Corticotropin Releasing Factor 2 Receptor." Molecular and Cellular Endocrinology, vol. 251, no. 1-2, 2006, pp. 103-11.
Gauna C, Delhanty PJ, van Aken MO, et al. Unacylated ghrelin is active on the INS-1E rat insulinoma cell line independently of the growth hormone secretagogue receptor type 1a and the corticotropin releasing factor 2 receptor. Mol Cell Endocrinol. 2006;251(1-2):103-11.
Gauna, C., Delhanty, P. J., van Aken, M. O., Janssen, J. A., Themmen, A. P., Hofland, L. J., Culler, M., Broglio, F., Ghigo, E., & van der Lely, A. J. (2006). Unacylated ghrelin is active on the INS-1E rat insulinoma cell line independently of the growth hormone secretagogue receptor type 1a and the corticotropin releasing factor 2 receptor. Molecular and Cellular Endocrinology, 251(1-2), 103-11.
Gauna C, et al. Unacylated Ghrelin Is Active On the INS-1E Rat Insulinoma Cell Line Independently of the Growth Hormone Secretagogue Receptor Type 1a and the Corticotropin Releasing Factor 2 Receptor. Mol Cell Endocrinol. 2006 Jun 7;251(1-2):103-11. PubMed PMID: 16647196.
TY - JOUR
T1 - Unacylated ghrelin is active on the INS-1E rat insulinoma cell line independently of the growth hormone secretagogue receptor type 1a and the corticotropin releasing factor 2 receptor.
AU - Gauna,Carlotta,
AU - Delhanty,Patric J D,
AU - van Aken,Maarten O,
AU - Janssen,Joop A M J L,
AU - Themmen,Axel P N,
AU - Hofland,Leo J,
AU - Culler,Michael,
AU - Broglio,Fabio,
AU - Ghigo,Ezio,
AU - van der Lely,Aart Jan,
Y1 - 2006/04/27/
PY - 2006/03/03/received
PY - 2006/03/28/revised
PY - 2006/03/28/accepted
PY - 2006/5/2/pubmed
PY - 2006/8/19/medline
PY - 2006/5/2/entrez
SP - 103
EP - 11
JF - Molecular and cellular endocrinology
JO - Mol Cell Endocrinol
VL - 251
IS - 1-2
N2 - Both unacylated ghrelin (UAG) and acylated ghrelin (AG) exert metabolic effects. To investigate the interactions between AG and UAG on ghrelin receptors we evaluated the effects of AG and UAG on INS-1E rat insulinoma cells, using insulin secretion after 30min static incubation as a read-out. A possible involvement of the growth hormone secretagogue receptor type 1a (GHS-R1a) or the corticotropin-releasing factor 2 (CRF2) receptor (CRF2R), as a putative receptor for UAG, was also studied determining their mRNA expression and the functional effects of receptor antagonists on insulin release. Both UAG and AG stimulated insulin release dose-dependently in the nanomolar range. The AG-induced insulin output was antagonized by two GHS-R1a antagonists ([d-Lys(3)]GHRP-6 and BIM28163), which did not block UAG actions. These effects occurred in the presence of low levels of GHS-R1a mRNA. Neither CRF2R expression nor effects of the CRF2R antagonist (astressin(2)B) on insulin output were observed. In conclusion, we provide a sensitive and reproducible assay for specific effects of UAG, which in this study is responsible for insulin release by INS-1E cells. Our data support the existence of a specific receptor for UAG, other than the CRF2R and GHS-R1a. The stimulatory effect on insulin secretion by AG in this cell line is mediated by the GHS-R1a.
SN - 0303-7207
UR - https://www.unboundmedicine.com/medline/citation/16647196/Unacylated_ghrelin_is_active_on_the_INS_1E_rat_insulinoma_cell_line_independently_of_the_growth_hormone_secretagogue_receptor_type_1a_and_the_corticotropin_releasing_factor_2_receptor_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0303-7207(06)00163-8
DB - PRIME
DP - Unbound Medicine
ER -