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[Development of a technique for recovering Giardia cysts and Cryptosporidium oocysts from fresh vegetables].
Ann Ig. 2006 Mar-Apr; 18(2):101-7.AI

Abstract

Techniques described for recovering Giardia and Cryptosporidium (oo)cysts from fruit and vegetables are generally inadequate and present variable recovery efficience and elevated costs. The aim of our study was to evaluate the recovery efficiency of a simple and economic technique to apply either to berry vegetables, like tomatoes and peppers, or to large leave vegetables, like lettuce and chicory. The method include contamination and further elution of the vegetables. Then sedimentation of (oo)cysts by centrifugation of the eluate of vegetables and their visualization by means of direct immunofluorescence. The higher recovery values for both protozoa were obtained in large leave vegetables with mean data above 70% for Giardia and 76% for Cryptosporidium, whereas the values observed in the berry vegetables were above 43% for Giardia and above 37% for Cryptosporidium on average.

Authors+Show Affiliations

Di Benedetto MA
Dipartimento di Igiene e Microbiologia, Università degli Studi di Palermo. dibenedetto@igiene.unipa.it
Di Piazza F
No affiliation info available
Oliveri R
No affiliation info available
Cerame G
No affiliation info available
Valenti R
No affiliation info available
Firenze A
No affiliation info available

MeSH

AnimalsCentrifugationCryptosporidiumFluorescent Antibody Technique, DirectFood ParasitologyGiardiaOocystsVegetables

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

ita

PubMed ID

16649507

Citation

Di Benedetto, M A., et al. "[Development of a Technique for Recovering Giardia Cysts and Cryptosporidium Oocysts From Fresh Vegetables]." Annali Di Igiene : Medicina Preventiva E Di Comunita, vol. 18, no. 2, 2006, pp. 101-7.
Di Benedetto MA, Di Piazza F, Oliveri R, et al. [Development of a technique for recovering Giardia cysts and Cryptosporidium oocysts from fresh vegetables]. Ann Ig. 2006;18(2):101-7.
Di Benedetto, M. A., Di Piazza, F., Oliveri, R., Cerame, G., Valenti, R., & Firenze, A. (2006). [Development of a technique for recovering Giardia cysts and Cryptosporidium oocysts from fresh vegetables]. Annali Di Igiene : Medicina Preventiva E Di Comunita, 18(2), 101-7.
Di Benedetto MA, et al. [Development of a Technique for Recovering Giardia Cysts and Cryptosporidium Oocysts From Fresh Vegetables]. Ann Ig. 2006 Mar-Apr;18(2):101-7. PubMed PMID: 16649507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Development of a technique for recovering Giardia cysts and Cryptosporidium oocysts from fresh vegetables]. AU - Di Benedetto,M A, AU - Di Piazza,F, AU - Oliveri,R, AU - Cerame,G, AU - Valenti,R, AU - Firenze,A, PY - 2006/5/3/pubmed PY - 2006/5/12/medline PY - 2006/5/3/entrez SP - 101 EP - 7 JF - Annali di igiene : medicina preventiva e di comunita JO - Ann Ig VL - 18 IS - 2 N2 - Techniques described for recovering Giardia and Cryptosporidium (oo)cysts from fruit and vegetables are generally inadequate and present variable recovery efficience and elevated costs. The aim of our study was to evaluate the recovery efficiency of a simple and economic technique to apply either to berry vegetables, like tomatoes and peppers, or to large leave vegetables, like lettuce and chicory. The method include contamination and further elution of the vegetables. Then sedimentation of (oo)cysts by centrifugation of the eluate of vegetables and their visualization by means of direct immunofluorescence. The higher recovery values for both protozoa were obtained in large leave vegetables with mean data above 70% for Giardia and 76% for Cryptosporidium, whereas the values observed in the berry vegetables were above 43% for Giardia and above 37% for Cryptosporidium on average. SN - 1120-9135 UR - https://www.unboundmedicine.com/medline/citation/16649507/[Development_of_a_technique_for_recovering_Giardia_cysts_and_Cryptosporidium_oocysts_from_fresh_vegetables]_ DB - PRIME DP - Unbound Medicine ER -