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The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area.
Clin Exp Allergy. 2006 May; 36(5):666-75.CE

Abstract

BACKGROUND

In contrast to other Rosaceae fruit, only few cases of patients with adverse reactions to strawberry are listed in literature. OBJECTIVE To identify allergenic proteins in strawberry and to express and characterize recombinant strawberry lipid transfer protein (LTP; rFra a 3).

METHODS

Established apple-allergic patients were recruited on the basis of a reported allergic reaction to strawberry (n=28, confirmed by double-blind placebo-controlled food challenge in four patients) or on the basis of IgE reactivity to LTP (n=34). Sensitization to purified natural and recombinant allergens was assessed by RAST, immunoblot (inhibition) and basophil histamine release (BHR). A strawberry cDNA library was screened for genes homologous to known fruit allergens. Fra a 3 was cloned and expressed in the yeast Pichia pastoris and compared with peach and apple LTP by RAST, immunoblot-inhibition and BHR tests.

RESULTS

Genes homologous to Bet v 1, Bet v 6, profilin and LTP were identified in a strawberry cDNA library. In BHR the rFra a 3 induced histamine release at a 100-fold higher concentration than peach LTP. RAST inhibition showed high cross-reactivity to peach and apple LTP, although IgE reactivity was lower by a factor 5. On strawberry immunoblot, patients' IgE showed reactivity to a Bet v 1 homologue, profilin, LTP and high-molecular weight bands.

CONCLUSION

In addition to a Bet v 1 homologue, strawberry also contains IgE-binding profilin and LTP. The rFra a 3 has less allergenic potency than peach and apple LTP, and therefore is an interesting tool for future immunotherapy. Fra a 3 does not seem to be clinically relevant.

Authors+Show Affiliations

Laboratory of Allergy Research, Department of Experimental Immunology, Amsterdam Medical Centre, Amsterdam, The Netherlands. l.zuidmeer@sanquin.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16650053

Citation

Zuidmeer, L, et al. "The Role of Profilin and Lipid Transfer Protein in Strawberry Allergy in the Mediterranean Area." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 36, no. 5, 2006, pp. 666-75.
Zuidmeer L, Salentijn E, Rivas MF, et al. The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area. Clin Exp Allergy. 2006;36(5):666-75.
Zuidmeer, L., Salentijn, E., Rivas, M. F., Mancebo, E. G., Asero, R., Matos, C. I., Pelgrom, K. T., Gilissen, L. J., & van Ree, R. (2006). The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 36(5), 666-75.
Zuidmeer L, et al. The Role of Profilin and Lipid Transfer Protein in Strawberry Allergy in the Mediterranean Area. Clin Exp Allergy. 2006;36(5):666-75. PubMed PMID: 16650053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area. AU - Zuidmeer,L, AU - Salentijn,E, AU - Rivas,M F, AU - Mancebo,E G, AU - Asero,R, AU - Matos,C I, AU - Pelgrom,K T B, AU - Gilissen,L J W J, AU - van Ree,R, PY - 2006/5/3/pubmed PY - 2006/10/28/medline PY - 2006/5/3/entrez SP - 666 EP - 75 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 36 IS - 5 N2 - BACKGROUND: In contrast to other Rosaceae fruit, only few cases of patients with adverse reactions to strawberry are listed in literature. OBJECTIVE To identify allergenic proteins in strawberry and to express and characterize recombinant strawberry lipid transfer protein (LTP; rFra a 3). METHODS: Established apple-allergic patients were recruited on the basis of a reported allergic reaction to strawberry (n=28, confirmed by double-blind placebo-controlled food challenge in four patients) or on the basis of IgE reactivity to LTP (n=34). Sensitization to purified natural and recombinant allergens was assessed by RAST, immunoblot (inhibition) and basophil histamine release (BHR). A strawberry cDNA library was screened for genes homologous to known fruit allergens. Fra a 3 was cloned and expressed in the yeast Pichia pastoris and compared with peach and apple LTP by RAST, immunoblot-inhibition and BHR tests. RESULTS: Genes homologous to Bet v 1, Bet v 6, profilin and LTP were identified in a strawberry cDNA library. In BHR the rFra a 3 induced histamine release at a 100-fold higher concentration than peach LTP. RAST inhibition showed high cross-reactivity to peach and apple LTP, although IgE reactivity was lower by a factor 5. On strawberry immunoblot, patients' IgE showed reactivity to a Bet v 1 homologue, profilin, LTP and high-molecular weight bands. CONCLUSION: In addition to a Bet v 1 homologue, strawberry also contains IgE-binding profilin and LTP. The rFra a 3 has less allergenic potency than peach and apple LTP, and therefore is an interesting tool for future immunotherapy. Fra a 3 does not seem to be clinically relevant. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/16650053/The_role_of_profilin_and_lipid_transfer_protein_in_strawberry_allergy_in_the_Mediterranean_area_ L2 - https://doi.org/10.1111/j.1365-2222.2006.02453.x DB - PRIME DP - Unbound Medicine ER -