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Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people.
Prostaglandins Leukot Essent Fatty Acids. 2006 Jun; 74(6):347-56.PL

Abstract

Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis. In this study, lymphocytes of diabetic type 2 were incubated with different polyunsaturated fatty acid (docosahexaenoic, eicosapentaenoic, arachidonic acid) for investigated their effect on lymphoproliferation response, the concentration of interleukin 2 produced in each essay and phospholipid fatty acid composition of lymphocyte membrane. Our results found that the concanavalin A and insulin increase significantly the proliferative response while eicosapentaenoic, arachidonic and docosahexaenoic acid inhibited that by different degrees: 47%, 37% and 19%, respectively, for healthy subjects and 39%, 29% and 13% for diabetes. However, the concentration of IL-2 produced in presence of either docosahexaenoic, eicosapentaenoic or arachidonic acid was significantly reduced by 36%, 32% and 39%, respectively, in controls while 16%, 15% and 23%, respectively, in diabetics. On the other hand, the tested fatty acids demonstrated a major impact on the fatty acid composition of different phospholipid fractions of lymphocyte membrane but these fractions were different in their response to each fatty acid examined. For instance, the addition of docosahexaenoic acid to culture media was accompanied with a predominant composition of docosahexaenoic acid in phospholipid fractions. Also, our results showed a notable increased proportion of arachidonic, eicosapentaenoic and docosahexaenoic acids in control phospholipid fractions than those of diabetic.

Authors+Show Affiliations

Laboratory of Cellular Biology, Department of Biology, University of Tlemcen, P.O. Box 119, Tlemcen 13000, Algeria. alnajjar_as@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16650969

Citation

Alnajjar, A, et al. "Effect of N-3 and N-6 Polyunsaturated Fatty Acids On Lymphocyte Proliferation, Interleukin Production and Phospholipid Fatty Acids Composition in Type 2 Diabetic and Healthy Subjects in Jordan People." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 74, no. 6, 2006, pp. 347-56.
Alnajjar A, Chabane Sari D, Abuharfeil N, et al. Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people. Prostaglandins Leukot Essent Fatty Acids. 2006;74(6):347-56.
Alnajjar, A., Chabane Sari, D., Abuharfeil, N., Hudaib, M., & Aburjai, T. (2006). Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 74(6), 347-56.
Alnajjar A, et al. Effect of N-3 and N-6 Polyunsaturated Fatty Acids On Lymphocyte Proliferation, Interleukin Production and Phospholipid Fatty Acids Composition in Type 2 Diabetic and Healthy Subjects in Jordan People. Prostaglandins Leukot Essent Fatty Acids. 2006;74(6):347-56. PubMed PMID: 16650969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people. AU - Alnajjar,A, AU - Chabane Sari,D, AU - Abuharfeil,N, AU - Hudaib,M, AU - Aburjai,T, Y1 - 2006/05/02/ PY - 2005/12/28/received PY - 2006/03/12/accepted PY - 2006/5/3/pubmed PY - 2006/8/15/medline PY - 2006/5/3/entrez SP - 347 EP - 56 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot Essent Fatty Acids VL - 74 IS - 6 N2 - Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis. In this study, lymphocytes of diabetic type 2 were incubated with different polyunsaturated fatty acid (docosahexaenoic, eicosapentaenoic, arachidonic acid) for investigated their effect on lymphoproliferation response, the concentration of interleukin 2 produced in each essay and phospholipid fatty acid composition of lymphocyte membrane. Our results found that the concanavalin A and insulin increase significantly the proliferative response while eicosapentaenoic, arachidonic and docosahexaenoic acid inhibited that by different degrees: 47%, 37% and 19%, respectively, for healthy subjects and 39%, 29% and 13% for diabetes. However, the concentration of IL-2 produced in presence of either docosahexaenoic, eicosapentaenoic or arachidonic acid was significantly reduced by 36%, 32% and 39%, respectively, in controls while 16%, 15% and 23%, respectively, in diabetics. On the other hand, the tested fatty acids demonstrated a major impact on the fatty acid composition of different phospholipid fractions of lymphocyte membrane but these fractions were different in their response to each fatty acid examined. For instance, the addition of docosahexaenoic acid to culture media was accompanied with a predominant composition of docosahexaenoic acid in phospholipid fractions. Also, our results showed a notable increased proportion of arachidonic, eicosapentaenoic and docosahexaenoic acids in control phospholipid fractions than those of diabetic. SN - 0952-3278 UR - https://www.unboundmedicine.com/medline/citation/16650969/Effect_of_n_3_and_n_6_polyunsaturated_fatty_acids_on_lymphocyte_proliferation_interleukin_production_and_phospholipid_fatty_acids_composition_in_type_2_diabetic_and_healthy_subjects_in_Jordan_people_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(06)00041-X DB - PRIME DP - Unbound Medicine ER -