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Cytokine modulation of the innate immune response in feline immunodeficiency virus-infected cats.
J Infect Dis. 2006 Jun 01; 193(11):1520-7.JI

Abstract

BACKGROUND

In vitro data suggest that innate immune function in human immunodeficiency virus type 1-infected patients is compromised; however, in vivo studies are lacking. Feline immunodeficiency virus (FIV) infection in cats provides an excellent model to explore innate immune function in vivo. The innate response against Listeria monocytogenes is well understood, making it a useful immune probe.

METHODS

Recombinant L. monocytogenes carrying eukaryotic expression plasmids for feline tumor necrosis factor (TNF)- alpha , interleukin (IL)-10, interferon (IFN)- gamma , and IL-15 were created to determine whether specific cytokines would modulate innate immune function. L. monocytogenes was delivered subcutaneously, and local lymph nodes were evaluated for size, cell subpopulations, and L. monocytogenes burden. Two months later, memory responses were evaluated by IFN- gamma enzyme-linked immunospot assay.

RESULTS

FIV-positive cats had significantly less lymph-node enlargement and a greater L. monocytogenes burden than FIV-negative control cats. TNF- alpha improved listericidal activity in FIV-negative control cats but not in FIV-positive cats, whereas IL-10 modestly reduced function in FIV-negative control cats. IFN- gamma improved memory responses but not clearance of L. monocytogenes. IL-15 improved innate function in FIV-positive cats and increased the percentage of natural killer cells.

CONCLUSIONS

Lentivirus infection impairs innate immune function in vivo, and IL-15 can significantly restore function. We hypothesize that altered dendritic-cell function and increased regulatory T cell activity may underlie the innate immune defect in HIV infection.

Authors+Show Affiliations

Dean GA
Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, 27606, USA. Gregg_Dean@ncsu.edu
LaVoy A
No affiliation info available
Yearley J
No affiliation info available
Stanton C
No affiliation info available

MeSH

AnimalsCatsCytokinesDisease Models, AnimalFemaleImmunity, InnateImmunodeficiency Virus, FelineImmunologic MemoryInterferon-gammaLentivirus InfectionsListeria monocytogenesListeriosisLymph NodesLymphocyte SubsetsMaleReverse Transcriptase Polymerase Chain Reaction

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16652280

Citation

Dean, Gregg A., et al. "Cytokine Modulation of the Innate Immune Response in Feline Immunodeficiency Virus-infected Cats." The Journal of Infectious Diseases, vol. 193, no. 11, 2006, pp. 1520-7.
Dean GA, LaVoy A, Yearley J, et al. Cytokine modulation of the innate immune response in feline immunodeficiency virus-infected cats. J Infect Dis. 2006;193(11):1520-7.
Dean, G. A., LaVoy, A., Yearley, J., & Stanton, C. (2006). Cytokine modulation of the innate immune response in feline immunodeficiency virus-infected cats. The Journal of Infectious Diseases, 193(11), 1520-7.
Dean GA, et al. Cytokine Modulation of the Innate Immune Response in Feline Immunodeficiency Virus-infected Cats. J Infect Dis. 2006 Jun 1;193(11):1520-7. PubMed PMID: 16652280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine modulation of the innate immune response in feline immunodeficiency virus-infected cats. AU - Dean,Gregg A, AU - LaVoy,Alora, AU - Yearley,Jennifer, AU - Stanton,Christine, Y1 - 2006/04/26/ PY - 2005/11/30/received PY - 2006/01/19/accepted PY - 2006/5/3/pubmed PY - 2006/6/23/medline PY - 2006/5/3/entrez SP - 1520 EP - 7 JF - The Journal of infectious diseases JO - J Infect Dis VL - 193 IS - 11 N2 - BACKGROUND: In vitro data suggest that innate immune function in human immunodeficiency virus type 1-infected patients is compromised; however, in vivo studies are lacking. Feline immunodeficiency virus (FIV) infection in cats provides an excellent model to explore innate immune function in vivo. The innate response against Listeria monocytogenes is well understood, making it a useful immune probe. METHODS: Recombinant L. monocytogenes carrying eukaryotic expression plasmids for feline tumor necrosis factor (TNF)- alpha , interleukin (IL)-10, interferon (IFN)- gamma , and IL-15 were created to determine whether specific cytokines would modulate innate immune function. L. monocytogenes was delivered subcutaneously, and local lymph nodes were evaluated for size, cell subpopulations, and L. monocytogenes burden. Two months later, memory responses were evaluated by IFN- gamma enzyme-linked immunospot assay. RESULTS: FIV-positive cats had significantly less lymph-node enlargement and a greater L. monocytogenes burden than FIV-negative control cats. TNF- alpha improved listericidal activity in FIV-negative control cats but not in FIV-positive cats, whereas IL-10 modestly reduced function in FIV-negative control cats. IFN- gamma improved memory responses but not clearance of L. monocytogenes. IL-15 improved innate function in FIV-positive cats and increased the percentage of natural killer cells. CONCLUSIONS: Lentivirus infection impairs innate immune function in vivo, and IL-15 can significantly restore function. We hypothesize that altered dendritic-cell function and increased regulatory T cell activity may underlie the innate immune defect in HIV infection. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/16652280/Cytokine_modulation_of_the_innate_immune_response_in_feline_immunodeficiency_virus_infected_cats_ DB - PRIME DP - Unbound Medicine ER -