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Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence.
BMC Genomics. 2006 May 02; 7:101.BG

Abstract

BACKGROUND

By comparing the quail genome with that of chicken, chromosome rearrangements that have occurred in these two galliform species over 35 million years of evolution can be detected. From a more practical point of view, the definition of conserved syntenies helps to predict the position of genes in quail, based on information taken from the chicken sequence, thus enhancing the utility of this species in biological studies through a better knowledge of its genome structure. A microsatellite and an Amplified Fragment Length Polymorphism (AFLP) genetic map were previously published for quail, as well as comparative cytogenetic data with chicken for macrochromosomes. Quail genomics will benefit from the extension and the integration of these maps.

RESULTS

The integrated linkage map presented here is based on segregation analysis of both anonymous markers and functional gene loci in 1,050 quail from three independent F2 populations. Ninety-two loci are resolved into 14 autosomal linkage groups and a Z chromosome-specific linkage group, aligned with the quail AFLP map. The size of linkage groups ranges from 7.8 cM to 274.8 cM. The total map distance covers 904.3 cM with an average spacing of 9.7 cM between loci. The coverage is not complete, as macrochromosome CJA08, the gonosome CJAW and 23 microchromosomes have no marker assigned yet. Significant sequence identities of quail markers with chicken enabled the alignment of the quail linkage groups on the chicken genome sequence assembly. This, together with interspecific Fluorescence In Situ Hybridization (FISH), revealed very high similarities in marker order between the two species for the eight macrochromosomes and the 14 microchromosomes studied.

CONCLUSION

Integrating the two microsatellite and the AFLP quail genetic maps greatly enhances the quality of the resulting information and will thus facilitate the identification of Quantitative Trait Loci (QTL). The alignment with the chicken chromosomes confirms the high conservation of gene order that was expected between the two species for macrochromosomes. By extending the comparative study to the microchromosomes, we suggest that a wealth of information can be mined in chicken, to be used for genome analyses in quail.

Authors+Show Affiliations

Laboratoire de Génétique Cellulaire, Centre INRA de Toulouse, BP 52627 Auzeville, 31326 Castanet Tolosan, France. bbkayang@ug.edu.ghNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16669996

Citation

Kayang, Boniface B., et al. "Integrated Maps in Quail (Coturnix Japonica) Confirm the High Degree of Synteny Conservation With Chicken (Gallus Gallus) Despite 35 Million Years of Divergence." BMC Genomics, vol. 7, 2006, p. 101.
Kayang BB, Fillon V, Inoue-Murayama M, et al. Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence. BMC Genomics. 2006;7:101.
Kayang, B. B., Fillon, V., Inoue-Murayama, M., Miwa, M., Leroux, S., Fève, K., Monvoisin, J. L., Pitel, F., Vignoles, M., Mouilhayrat, C., Beaumont, C., Ito, S., Minvielle, F., & Vignal, A. (2006). Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence. BMC Genomics, 7, 101.
Kayang BB, et al. Integrated Maps in Quail (Coturnix Japonica) Confirm the High Degree of Synteny Conservation With Chicken (Gallus Gallus) Despite 35 Million Years of Divergence. BMC Genomics. 2006 May 2;7:101. PubMed PMID: 16669996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence. AU - Kayang,Boniface B, AU - Fillon,Valérie, AU - Inoue-Murayama,Miho, AU - Miwa,Mitsuru, AU - Leroux,Sophie, AU - Fève,Katia, AU - Monvoisin,Jean-Louis, AU - Pitel,Frédérique, AU - Vignoles,Matthieu, AU - Mouilhayrat,Céline, AU - Beaumont,Catherine, AU - Ito,Shin'ichi, AU - Minvielle,Francis, AU - Vignal,Alain, Y1 - 2006/05/02/ PY - 2005/12/08/received PY - 2006/05/02/accepted PY - 2006/5/4/pubmed PY - 2006/10/13/medline PY - 2006/5/4/entrez SP - 101 EP - 101 JF - BMC genomics JO - BMC Genomics VL - 7 N2 - BACKGROUND: By comparing the quail genome with that of chicken, chromosome rearrangements that have occurred in these two galliform species over 35 million years of evolution can be detected. From a more practical point of view, the definition of conserved syntenies helps to predict the position of genes in quail, based on information taken from the chicken sequence, thus enhancing the utility of this species in biological studies through a better knowledge of its genome structure. A microsatellite and an Amplified Fragment Length Polymorphism (AFLP) genetic map were previously published for quail, as well as comparative cytogenetic data with chicken for macrochromosomes. Quail genomics will benefit from the extension and the integration of these maps. RESULTS: The integrated linkage map presented here is based on segregation analysis of both anonymous markers and functional gene loci in 1,050 quail from three independent F2 populations. Ninety-two loci are resolved into 14 autosomal linkage groups and a Z chromosome-specific linkage group, aligned with the quail AFLP map. The size of linkage groups ranges from 7.8 cM to 274.8 cM. The total map distance covers 904.3 cM with an average spacing of 9.7 cM between loci. The coverage is not complete, as macrochromosome CJA08, the gonosome CJAW and 23 microchromosomes have no marker assigned yet. Significant sequence identities of quail markers with chicken enabled the alignment of the quail linkage groups on the chicken genome sequence assembly. This, together with interspecific Fluorescence In Situ Hybridization (FISH), revealed very high similarities in marker order between the two species for the eight macrochromosomes and the 14 microchromosomes studied. CONCLUSION: Integrating the two microsatellite and the AFLP quail genetic maps greatly enhances the quality of the resulting information and will thus facilitate the identification of Quantitative Trait Loci (QTL). The alignment with the chicken chromosomes confirms the high conservation of gene order that was expected between the two species for macrochromosomes. By extending the comparative study to the microchromosomes, we suggest that a wealth of information can be mined in chicken, to be used for genome analyses in quail. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/16669996/Integrated_maps_in_quail__Coturnix_japonica__confirm_the_high_degree_of_synteny_conservation_with_chicken__Gallus_gallus__despite_35_million_years_of_divergence_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-7-101 DB - PRIME DP - Unbound Medicine ER -