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A validated chiral CE method for Frovatriptan, using cyclodextrin as chiral selector.
J Pharm Biomed Anal. 2006 Jun 16; 41(4):1447-52.JP

Abstract

A cyclodextrin modified capillary zone electrophoretic method has been developed for the evaluation of chiral purity of Frovatriptan using sulfobutyl ether beta cyclodextrin (SB-beta-CD) as the chiral selector. The method is highly specific, accurate and reproducible. The method was optimized with a systematic method development approach by optimizing the pH of electrolyte, attempting the separation in different classes of chiral selectors and modifying parameters such as cyclodextrin concentration and the organic modifier type and concentration. The optimized method was validated for specificity, precision, linearity, accuracy and stability in solution using Imidazole as the internal standard. The limit of detection (LOD) and limit of quantification (LOQ) were 1.0 microg/mL and 5.0 microg/mL respectively for each isomer. The method was applied for estimating the chiral purity of various batches of Frovatriptan.

Authors+Show Affiliations

Khan M
Department of Analytical Research, Dr. Reddy's Laboratories Ltd., Discovery Research, Bollaram Road, Miyapur, Hyderabad 500 050, Andhra Pradesh, India. muzaffark@drreddys.com
Viswanathan B
No affiliation info available
Rao DS
No affiliation info available
Reddy GS
No affiliation info available

MeSH

CarbazolesCyclodextrinsElectrophoresis, CapillaryHydrogen-Ion ConcentrationSerotonin Receptor AgonistsStereoisomerismTryptamines

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16672183

Citation

Khan, Muzaffar, et al. "A Validated Chiral CE Method for Frovatriptan, Using Cyclodextrin as Chiral Selector." Journal of Pharmaceutical and Biomedical Analysis, vol. 41, no. 4, 2006, pp. 1447-52.
Khan M, Viswanathan B, Rao DS, et al. A validated chiral CE method for Frovatriptan, using cyclodextrin as chiral selector. J Pharm Biomed Anal. 2006;41(4):1447-52.
Khan, M., Viswanathan, B., Rao, D. S., & Reddy, G. S. (2006). A validated chiral CE method for Frovatriptan, using cyclodextrin as chiral selector. Journal of Pharmaceutical and Biomedical Analysis, 41(4), 1447-52.
Khan M, et al. A Validated Chiral CE Method for Frovatriptan, Using Cyclodextrin as Chiral Selector. J Pharm Biomed Anal. 2006 Jun 16;41(4):1447-52. PubMed PMID: 16672183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A validated chiral CE method for Frovatriptan, using cyclodextrin as chiral selector. AU - Khan,Muzaffar, AU - Viswanathan,Balaji, AU - Rao,D Sreenivas, AU - Reddy,G Sreenivas, Y1 - 2006/05/02/ PY - 2005/09/16/received PY - 2006/03/08/revised PY - 2006/03/09/accepted PY - 2006/5/5/pubmed PY - 2006/8/3/medline PY - 2006/5/5/entrez SP - 1447 EP - 52 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 41 IS - 4 N2 - A cyclodextrin modified capillary zone electrophoretic method has been developed for the evaluation of chiral purity of Frovatriptan using sulfobutyl ether beta cyclodextrin (SB-beta-CD) as the chiral selector. The method is highly specific, accurate and reproducible. The method was optimized with a systematic method development approach by optimizing the pH of electrolyte, attempting the separation in different classes of chiral selectors and modifying parameters such as cyclodextrin concentration and the organic modifier type and concentration. The optimized method was validated for specificity, precision, linearity, accuracy and stability in solution using Imidazole as the internal standard. The limit of detection (LOD) and limit of quantification (LOQ) were 1.0 microg/mL and 5.0 microg/mL respectively for each isomer. The method was applied for estimating the chiral purity of various batches of Frovatriptan. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16672183/A_validated_chiral_CE_method_for_Frovatriptan_using_cyclodextrin_as_chiral_selector_ DB - PRIME DP - Unbound Medicine ER -