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A host factor absent from Lactococcus lactis subspecies lactis MG1363 is required for conjugative transposition.
Mol Microbiol 1991; 5(12):2983-93MM

Abstract

In matings between Lactococcus lactis strains, the conjugative transposons Tn916 and Tn919 are found in the chromosome of the transconjugants in the same place as in the chromosome of the donor, indicating that no transposition has occurred. In agreement with this, the frequency of L. lactis transconjugants from intraspecies matings is the same whether the donor contains the wild-type form of the transposon or the mutant Tn916-int1, which has an insertion in the transposon's integrase gene. However, in intergeneric crosses with Bacillus subtilis or Enterococcus faecalis donors, Tn916 and Tn919 transpose to different locations on the chromosome of the L. lactis transconjugants. Moreover, Tn916 and Tn919 could not be transferred by conjugation from L. lactis and B. subtilis, E. faecalis or Streptococcus pyogenes. This suggests that excision of these elements does not occur in L. lactis. When cloned into E. coli with adjacent chromosomal DNA from L. lactis, the conjugative transposons were able to excise, transpose and promote conjugation. Therefore, the inability of these elements to excise in L. lactis is not caused by a permanent structural alteration in the transposon. We conclude that L. lactis lacks a factor required for excision of conjugative transposons.

Authors+Show Affiliations

Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1667220

Citation

Bringel, F, et al. "A Host Factor Absent From Lactococcus Lactis Subspecies Lactis MG1363 Is Required for Conjugative Transposition." Molecular Microbiology, vol. 5, no. 12, 1991, pp. 2983-93.
Bringel F, Van Alstine GL, Scott JR. A host factor absent from Lactococcus lactis subspecies lactis MG1363 is required for conjugative transposition. Mol Microbiol. 1991;5(12):2983-93.
Bringel, F., Van Alstine, G. L., & Scott, J. R. (1991). A host factor absent from Lactococcus lactis subspecies lactis MG1363 is required for conjugative transposition. Molecular Microbiology, 5(12), pp. 2983-93.
Bringel F, Van Alstine GL, Scott JR. A Host Factor Absent From Lactococcus Lactis Subspecies Lactis MG1363 Is Required for Conjugative Transposition. Mol Microbiol. 1991;5(12):2983-93. PubMed PMID: 1667220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A host factor absent from Lactococcus lactis subspecies lactis MG1363 is required for conjugative transposition. AU - Bringel,F, AU - Van Alstine,G L, AU - Scott,J R, PY - 1991/12/1/pubmed PY - 1991/12/1/medline PY - 1991/12/1/entrez SP - 2983 EP - 93 JF - Molecular microbiology JO - Mol. Microbiol. VL - 5 IS - 12 N2 - In matings between Lactococcus lactis strains, the conjugative transposons Tn916 and Tn919 are found in the chromosome of the transconjugants in the same place as in the chromosome of the donor, indicating that no transposition has occurred. In agreement with this, the frequency of L. lactis transconjugants from intraspecies matings is the same whether the donor contains the wild-type form of the transposon or the mutant Tn916-int1, which has an insertion in the transposon's integrase gene. However, in intergeneric crosses with Bacillus subtilis or Enterococcus faecalis donors, Tn916 and Tn919 transpose to different locations on the chromosome of the L. lactis transconjugants. Moreover, Tn916 and Tn919 could not be transferred by conjugation from L. lactis and B. subtilis, E. faecalis or Streptococcus pyogenes. This suggests that excision of these elements does not occur in L. lactis. When cloned into E. coli with adjacent chromosomal DNA from L. lactis, the conjugative transposons were able to excise, transpose and promote conjugation. Therefore, the inability of these elements to excise in L. lactis is not caused by a permanent structural alteration in the transposon. We conclude that L. lactis lacks a factor required for excision of conjugative transposons. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/1667220/A_host_factor_absent_from_Lactococcus_lactis_subspecies_lactis_MG1363_is_required_for_conjugative_transposition_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1991&volume=5&issue=12&spage=2983 DB - PRIME DP - Unbound Medicine ER -