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Convenient structural analysis of glycosphingolipids using MALDI-QIT-TOF mass spectrometry with increased laser power and cooling gas flow.
J Biochem. 2006 Apr; 139(4):771-7.JB

Abstract

Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) was applied to the structural characterization of neutral glycosphingolipids. Lithium adduct ions of glycosphingolipids were analyzed using MALDI-QIT-TOF MS under strong conditions of increased laser power and cooling gas flow. The relative intensities of fragment ions were increased under the strong conditions, and the resulting spectra revealed the presence of oligosaccharide ions fragmented from the glycosphingolipids. Consequently, the oligosaccharide sequences of the glycosphingolipids were readily obtained. To obtain more detailed structural information, MS/MS (MS2) and MS/MS/MS (MS3) analyses were performed with selection of the lactosylceramide and ceramide ions, respectively. The resulting data were sufficient to determine the structures of both the oligosaccharide and the ceramide moiety of each glycosphingolipid. The fragmentation patterns of MS2 and MS3 for Forssman glycolipid under the strong conditions were comparable to those of MS3 and MS4 obtained under standard conditions, respectively. Thus, MALDI-QIT-TOF MS with increased laser power and cooling gas flow is a convenient method for glycosphingolipid analysis.

Authors+Show Affiliations

Sphingolipid Expression Laboratory, RIKEN Frontier Research System, Wako, Saitama 351-0198.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16672278

Citation

Suzuki, Yusuke, et al. "Convenient Structural Analysis of Glycosphingolipids Using MALDI-QIT-TOF Mass Spectrometry With Increased Laser Power and Cooling Gas Flow." Journal of Biochemistry, vol. 139, no. 4, 2006, pp. 771-7.
Suzuki Y, Suzuki M, Ito E, et al. Convenient structural analysis of glycosphingolipids using MALDI-QIT-TOF mass spectrometry with increased laser power and cooling gas flow. J Biochem. 2006;139(4):771-7.
Suzuki, Y., Suzuki, M., Ito, E., Goto-Inoue, N., Miseki, K., Iida, J., Yamazaki, Y., Yamada, M., & Suzuki, A. (2006). Convenient structural analysis of glycosphingolipids using MALDI-QIT-TOF mass spectrometry with increased laser power and cooling gas flow. Journal of Biochemistry, 139(4), 771-7.
Suzuki Y, et al. Convenient Structural Analysis of Glycosphingolipids Using MALDI-QIT-TOF Mass Spectrometry With Increased Laser Power and Cooling Gas Flow. J Biochem. 2006;139(4):771-7. PubMed PMID: 16672278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Convenient structural analysis of glycosphingolipids using MALDI-QIT-TOF mass spectrometry with increased laser power and cooling gas flow. AU - Suzuki,Yusuke, AU - Suzuki,Minoru, AU - Ito,Emi, AU - Goto-Inoue,Naoko, AU - Miseki,Kozo, AU - Iida,Junko, AU - Yamazaki,Yuzo, AU - Yamada,Masaki, AU - Suzuki,Akemi, PY - 2006/5/5/pubmed PY - 2006/7/14/medline PY - 2006/5/5/entrez SP - 771 EP - 7 JF - Journal of biochemistry JO - J Biochem VL - 139 IS - 4 N2 - Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) was applied to the structural characterization of neutral glycosphingolipids. Lithium adduct ions of glycosphingolipids were analyzed using MALDI-QIT-TOF MS under strong conditions of increased laser power and cooling gas flow. The relative intensities of fragment ions were increased under the strong conditions, and the resulting spectra revealed the presence of oligosaccharide ions fragmented from the glycosphingolipids. Consequently, the oligosaccharide sequences of the glycosphingolipids were readily obtained. To obtain more detailed structural information, MS/MS (MS2) and MS/MS/MS (MS3) analyses were performed with selection of the lactosylceramide and ceramide ions, respectively. The resulting data were sufficient to determine the structures of both the oligosaccharide and the ceramide moiety of each glycosphingolipid. The fragmentation patterns of MS2 and MS3 for Forssman glycolipid under the strong conditions were comparable to those of MS3 and MS4 obtained under standard conditions, respectively. Thus, MALDI-QIT-TOF MS with increased laser power and cooling gas flow is a convenient method for glycosphingolipid analysis. SN - 0021-924X UR - https://www.unboundmedicine.com/medline/citation/16672278/Convenient_structural_analysis_of_glycosphingolipids_using_MALDI_QIT_TOF_mass_spectrometry_with_increased_laser_power_and_cooling_gas_flow_ L2 - https://academic.oup.com/jb/article-lookup/doi/10.1093/jb/mvj090 DB - PRIME DP - Unbound Medicine ER -