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The role of tumor necrosis factor-alpha in the neuropathic pain induced by Lumbar 5 ventral root transection in rat.
Pain. 2006 Aug; 123(3):306-321.PAIN

Abstract

Accumulating evidence has demonstrated that tumor necrosis factor-alpha (TNF-alpha) plays an important role in neuropathic pain. Recently, it has been shown that Lumbar 5 ventral root transection (L5 VRT) induces persistent mechanical allodynia and thermal hyperalgesia in bilateral hind paws. In the present study, the role of TNF-alpha in the L5 VRT model was investigated. We found that immunoreactivity (IR) of TNF-alpha and TNF receptor 1 (TNFR1) in ipsilateral (but not in contralateral) L4 and L5 dorsal root ganglion (DRG) was increased following L5 VRT, started 1 day after the lesion and persisted for 2 weeks. Double immunofluorescence staining revealed that the increased TNF-alpha-IR in DRG was in satellite glial cells, immune cells and neuronal cells, while TNFR1-IR was almost restricted at DRG neuronal cells. L5 VRT increased TNF-alpha-IR and TNFR1-IR in bilateral L5 spinal dorsal horn, started 1 day after lesion and persisted for 2 weeks. The increased TNF-alpha-IR in spinal dorsal horn was observed in astrocytes, microglias and neurons, but the upregulation of TNFR1 was mainly in neurons. Intraperitoneal injection of thalidomide, an inhibitor of TNF-alpha synthesis, started at 2h before surgery, blocked mechanical allodynia and thermal hyperalgesia. However, the drug failed to reverse the abnormal pain behaviors, when it was applied at day 7 after surgery. These data suggest that the upregulation of TNF-alpha and TNFR1 in DRG and spinal dorsal horn is essential for the initiation but not for maintenance of the neuropathic pain induced by L5 VRT.

Authors+Show Affiliations

Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, 74 Zhongshan Rd. 2, Guangzhou 510080, P.R. China Department of Immunology, Zhongshan Medical School of Sun Yat-Sen University, 74 Zhongshan Rd. 2, Guangzhou 510080, P.R. China Pain Research Center of Sun Yat-Sen University, 74 Zhongshan Rd. 2, Guangzhou 510080, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16675114

Citation

Xu, Ji-Tian, et al. "The Role of Tumor Necrosis Factor-alpha in the Neuropathic Pain Induced By Lumbar 5 Ventral Root Transection in Rat." Pain, vol. 123, no. 3, 2006, pp. 306-321.
Xu JT, Xin WJ, Zang Y, et al. The role of tumor necrosis factor-alpha in the neuropathic pain induced by Lumbar 5 ventral root transection in rat. Pain. 2006;123(3):306-321.
Xu, J. T., Xin, W. J., Zang, Y., Wu, C. Y., & Liu, X. G. (2006). The role of tumor necrosis factor-alpha in the neuropathic pain induced by Lumbar 5 ventral root transection in rat. Pain, 123(3), 306-321. https://doi.org/10.1016/j.pain.2006.03.011
Xu JT, et al. The Role of Tumor Necrosis Factor-alpha in the Neuropathic Pain Induced By Lumbar 5 Ventral Root Transection in Rat. Pain. 2006;123(3):306-321. PubMed PMID: 16675114.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of tumor necrosis factor-alpha in the neuropathic pain induced by Lumbar 5 ventral root transection in rat. AU - Xu,Ji-Tian, AU - Xin,Wen-Jun, AU - Zang,Ying, AU - Wu,Chang-You, AU - Liu,Xian-Guo, Y1 - 2006/05/03/ PY - 2005/09/17/received PY - 2006/02/26/revised PY - 2006/03/13/accepted PY - 2006/5/6/pubmed PY - 2006/8/10/medline PY - 2006/5/6/entrez SP - 306 EP - 321 JF - Pain JO - Pain VL - 123 IS - 3 N2 - Accumulating evidence has demonstrated that tumor necrosis factor-alpha (TNF-alpha) plays an important role in neuropathic pain. Recently, it has been shown that Lumbar 5 ventral root transection (L5 VRT) induces persistent mechanical allodynia and thermal hyperalgesia in bilateral hind paws. In the present study, the role of TNF-alpha in the L5 VRT model was investigated. We found that immunoreactivity (IR) of TNF-alpha and TNF receptor 1 (TNFR1) in ipsilateral (but not in contralateral) L4 and L5 dorsal root ganglion (DRG) was increased following L5 VRT, started 1 day after the lesion and persisted for 2 weeks. Double immunofluorescence staining revealed that the increased TNF-alpha-IR in DRG was in satellite glial cells, immune cells and neuronal cells, while TNFR1-IR was almost restricted at DRG neuronal cells. L5 VRT increased TNF-alpha-IR and TNFR1-IR in bilateral L5 spinal dorsal horn, started 1 day after lesion and persisted for 2 weeks. The increased TNF-alpha-IR in spinal dorsal horn was observed in astrocytes, microglias and neurons, but the upregulation of TNFR1 was mainly in neurons. Intraperitoneal injection of thalidomide, an inhibitor of TNF-alpha synthesis, started at 2h before surgery, blocked mechanical allodynia and thermal hyperalgesia. However, the drug failed to reverse the abnormal pain behaviors, when it was applied at day 7 after surgery. These data suggest that the upregulation of TNF-alpha and TNFR1 in DRG and spinal dorsal horn is essential for the initiation but not for maintenance of the neuropathic pain induced by L5 VRT. SN - 1872-6623 UR - https://www.unboundmedicine.com/medline/citation/16675114/The_role_of_tumor_necrosis_factor_alpha_in_the_neuropathic_pain_induced_by_Lumbar_5_ventral_root_transection_in_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/00006396-200608000-00012 DB - PRIME DP - Unbound Medicine ER -