Tags

Type your tag names separated by a space and hit enter

Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode.
Biosens Bioelectron. 2007 Jan 15; 22(6):838-44.BB

Abstract

A new strategy for fabricating glucose biosensor was presented by layer-by-layer assembled chitosan (CS)/gold nanoparticles (GNp)/glucose oxidase (GOD) multilayer films modified Pt electrode. First, a cleaned Pt electrode was immersed in poly(allylamine) (PAA), and then transferred to GNp, followed by the adsorption of GOD (GOD/GNp/PAA/Pt). Second, the GOD/GNp/PAA/Pt electrode was immersed in CS, and then transferred to GNp, followed by the adsorption of GOD (GOD/GNp/CS/GOD/GNp/PAA/Pt). Third, different layers of multilayer films modified Pt electrodes were assembled by repeating the second process. Film assembling and characterization were studied by quart crystal microbalance, and properties of the resulting glucose biosensors were measured by electrochemical measurements. The results confirmed that the assembling process of multilayer films was simple to operate, the immobilized GOD displayed an excellent catalytic property to glucose, and GNp in the biosensing interface efficiently improved the electron transfer between analyte and electrode surface. The amperometric response of the biosensors uniformly increased from one to six layers of multilayer films, and then reached saturation after the seven layers. Among the resulting biosensors, the biosensor based on the six layers of multilayer films was best. It showed a wide linear range of 0.5-16 mM, with a detection limit of 7.0 microM estimated at a signal-to-noise ratio of 3, fast response time (within 8s). Moreover, it exhibited good reproducibility, long-term stability and interference free. This method can be used for constructing other thin films, which is a universal immobilization method for biosensor fabrication.

Authors+Show Affiliations

The key Laboratory of Bioactive Materials Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16675215

Citation

Wu, Bao-Yan, et al. "Amperometric Glucose Biosensor Based On Layer-by-layer Assembly of Multilayer Films Composed of Chitosan, Gold Nanoparticles and Glucose Oxidase Modified Pt Electrode." Biosensors & Bioelectronics, vol. 22, no. 6, 2007, pp. 838-44.
Wu BY, Hou SH, Yin F, et al. Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode. Biosens Bioelectron. 2007;22(6):838-44.
Wu, B. Y., Hou, S. H., Yin, F., Li, J., Zhao, Z. X., Huang, J. D., & Chen, Q. (2007). Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode. Biosensors & Bioelectronics, 22(6), 838-44.
Wu BY, et al. Amperometric Glucose Biosensor Based On Layer-by-layer Assembly of Multilayer Films Composed of Chitosan, Gold Nanoparticles and Glucose Oxidase Modified Pt Electrode. Biosens Bioelectron. 2007 Jan 15;22(6):838-44. PubMed PMID: 16675215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode. AU - Wu,Bao-Yan, AU - Hou,Shi-Hua, AU - Yin,Feng, AU - Li,Jing, AU - Zhao,Zi-Xia, AU - Huang,Jia-Dong, AU - Chen,Qiang, Y1 - 2006/05/03/ PY - 2005/12/03/received PY - 2006/02/17/revised PY - 2006/03/08/accepted PY - 2006/5/6/pubmed PY - 2007/3/7/medline PY - 2006/5/6/entrez SP - 838 EP - 44 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 6 N2 - A new strategy for fabricating glucose biosensor was presented by layer-by-layer assembled chitosan (CS)/gold nanoparticles (GNp)/glucose oxidase (GOD) multilayer films modified Pt electrode. First, a cleaned Pt electrode was immersed in poly(allylamine) (PAA), and then transferred to GNp, followed by the adsorption of GOD (GOD/GNp/PAA/Pt). Second, the GOD/GNp/PAA/Pt electrode was immersed in CS, and then transferred to GNp, followed by the adsorption of GOD (GOD/GNp/CS/GOD/GNp/PAA/Pt). Third, different layers of multilayer films modified Pt electrodes were assembled by repeating the second process. Film assembling and characterization were studied by quart crystal microbalance, and properties of the resulting glucose biosensors were measured by electrochemical measurements. The results confirmed that the assembling process of multilayer films was simple to operate, the immobilized GOD displayed an excellent catalytic property to glucose, and GNp in the biosensing interface efficiently improved the electron transfer between analyte and electrode surface. The amperometric response of the biosensors uniformly increased from one to six layers of multilayer films, and then reached saturation after the seven layers. Among the resulting biosensors, the biosensor based on the six layers of multilayer films was best. It showed a wide linear range of 0.5-16 mM, with a detection limit of 7.0 microM estimated at a signal-to-noise ratio of 3, fast response time (within 8s). Moreover, it exhibited good reproducibility, long-term stability and interference free. This method can be used for constructing other thin films, which is a universal immobilization method for biosensor fabrication. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16675215/Amperometric_glucose_biosensor_based_on_layer_by_layer_assembly_of_multilayer_films_composed_of_chitosan_gold_nanoparticles_and_glucose_oxidase_modified_Pt_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00102-3 DB - PRIME DP - Unbound Medicine ER -